Rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors
The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sector...
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Online Access: | http://hdl.handle.net/11368/2930096 https://doi.org/10.3390/catal8100471 https://www.mdpi.com/2073-4344/8/10/471 |
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ftunitriestiris:oai:arts.units.it:11368/2930096 2023-05-15T13:49:29+02:00 Rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors Marco Cespugli Simone Lotteria Luciano Navarini LONZARICH, VALENTINA Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi Cespugli, Marco Simone, Lotteria Navarini, Luciano Lonzarich, Valentina DEL TERRA, Lorenzo Vita, Francesca Zweyer, Marina Baldini, Giovanna Ferrario, Valerio Ebert, Cynthia Gardossi, Lucia 2018 ELETTRONICO http://hdl.handle.net/11368/2930096 https://doi.org/10.3390/catal8100471 https://www.mdpi.com/2073-4344/8/10/471 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000448543300067 volume:8 issue:10 firstpage:"-" lastpage:"-" numberofpages:23 journal:CATALYSTS http://hdl.handle.net/11368/2930096 doi:10.3390/catal8100471 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85055686474 https://www.mdpi.com/2073-4344/8/10/471 info:eu-repo/semantics/openAccess covalent immobilization of enzyme rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass info:eu-repo/semantics/article 2018 ftunitriestiris https://doi.org/10.3390/catal8100471 2023-04-09T06:18:21Z The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sectors where the covalent anchorage of the protein is a pre-requisite for preventing protein contamination while assuring the recyclability. Rice husk was oxidized and then functionalized with a di-amino spacer. The morphological characterization shed light on the properties that affect the functionalization processes. Lipase B from Candida antarctica (CaLB) and two commercial asparaginases were immobilized covalently achieving higher immobilization yield than previously reported. All enzymes were immobilized also on commercial epoxy methacrylic resins and the CaLB immobilized on rice husk demonstrated a higher efficiency in the solvent-free polycondensation of dimethylitaconate. CaLB on rice husk appears particularly suitable for applications in highly viscous processes because of the unusual combination of its low density and remarkable mechanical robustness. In the case of the two asparaginases, the biocatalyst immobilized on rice husk performed in aqueous solution at least as efficiently as the enzyme immobilized on methacrylic resins, although the rice husk loaded a lower amount of protein. Article in Journal/Newspaper Antarc* Antarctica Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) Anchorage Catalysts 8 10 471 |
institution |
Open Polar |
collection |
Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
op_collection_id |
ftunitriestiris |
language |
English |
topic |
covalent immobilization of enzyme rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass |
spellingShingle |
covalent immobilization of enzyme rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass Marco Cespugli Simone Lotteria Luciano Navarini LONZARICH, VALENTINA Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi Rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
topic_facet |
covalent immobilization of enzyme rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass |
description |
The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sectors where the covalent anchorage of the protein is a pre-requisite for preventing protein contamination while assuring the recyclability. Rice husk was oxidized and then functionalized with a di-amino spacer. The morphological characterization shed light on the properties that affect the functionalization processes. Lipase B from Candida antarctica (CaLB) and two commercial asparaginases were immobilized covalently achieving higher immobilization yield than previously reported. All enzymes were immobilized also on commercial epoxy methacrylic resins and the CaLB immobilized on rice husk demonstrated a higher efficiency in the solvent-free polycondensation of dimethylitaconate. CaLB on rice husk appears particularly suitable for applications in highly viscous processes because of the unusual combination of its low density and remarkable mechanical robustness. In the case of the two asparaginases, the biocatalyst immobilized on rice husk performed in aqueous solution at least as efficiently as the enzyme immobilized on methacrylic resins, although the rice husk loaded a lower amount of protein. |
author2 |
Cespugli, Marco Simone, Lotteria Navarini, Luciano Lonzarich, Valentina DEL TERRA, Lorenzo Vita, Francesca Zweyer, Marina Baldini, Giovanna Ferrario, Valerio Ebert, Cynthia Gardossi, Lucia |
format |
Article in Journal/Newspaper |
author |
Marco Cespugli Simone Lotteria Luciano Navarini LONZARICH, VALENTINA Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi |
author_facet |
Marco Cespugli Simone Lotteria Luciano Navarini LONZARICH, VALENTINA Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi |
author_sort |
Marco Cespugli |
title |
Rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
title_short |
Rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
title_full |
Rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
title_fullStr |
Rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
title_full_unstemmed |
Rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
title_sort |
rice husk as inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
publishDate |
2018 |
url |
http://hdl.handle.net/11368/2930096 https://doi.org/10.3390/catal8100471 https://www.mdpi.com/2073-4344/8/10/471 |
geographic |
Anchorage |
geographic_facet |
Anchorage |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000448543300067 volume:8 issue:10 firstpage:"-" lastpage:"-" numberofpages:23 journal:CATALYSTS http://hdl.handle.net/11368/2930096 doi:10.3390/catal8100471 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85055686474 https://www.mdpi.com/2073-4344/8/10/471 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/catal8100471 |
container_title |
Catalysts |
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8 |
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10 |
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471 |
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1766251441080500224 |