Identification of antibacterial peptides from endophytic microbiome
Endophytes, microorganisms living inside plant tissues, are promising producers of lead compounds for the pharmaceutical industry. However, the majority of endophytes are unculturable and therefore inaccessible for functional studies. To evaluate genetic resources of endophytes, we analyzed the biod...
Published in: | Applied Microbiology and Biotechnology |
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Online Access: | http://hdl.handle.net/11368/2906611 https://doi.org/10.1007/s00253-016-7765-4 https://link.springer.com/article/10.1007%2Fs00253-016-7765-4 |
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ftunitriestiris:oai:arts.units.it:11368/2906611 2023-05-15T15:59:28+02:00 Identification of antibacterial peptides from endophytic microbiome Tejesvi, M. V Picart, P. Kajula, M. Hautajärvi, H. Ruddock, L. Kristensen, H. H. Sahl, H. G. Ek, S. Mattila, S. Pirttilä, A. M. TOSSI, ALESSANDRO Tejesvi, M. V Picart, P. Kajula, M. Hautajärvi, H. Ruddock, L. Kristensen, H. H. Tossi, Alessandro Sahl, H. G. Ek, S. Mattila, S. Pirttilä, A. M. 2016 http://hdl.handle.net/11368/2906611 https://doi.org/10.1007/s00253-016-7765-4 https://link.springer.com/article/10.1007%2Fs00253-016-7765-4 eng eng info:eu-repo/semantics/altIdentifier/pmid/27541748 info:eu-repo/semantics/altIdentifier/wos/WOS:000385129600024 volume:100 issue:21 firstpage:9283 lastpage:9293 numberofpages:11 journal:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY http://hdl.handle.net/11368/2906611 doi:10.1007/s00253-016-7765-4 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84982273161 https://link.springer.com/article/10.1007%2Fs00253-016-7765-4 info:eu-repo/semantics/closedAccess Antimicrobial peptide Endophyte Microbiome Unculturable Anti-Bacterial Agent Ericaceae Escherichia coli Fungi Genetic Testing Peptide Staphylococcus aureu Biotechnology Applied Microbiology and Biotechnology info:eu-repo/semantics/article 2016 ftunitriestiris https://doi.org/10.1007/s00253-016-7765-4 2023-04-09T06:14:44Z Endophytes, microorganisms living inside plant tissues, are promising producers of lead compounds for the pharmaceutical industry. However, the majority of endophytes are unculturable and therefore inaccessible for functional studies. To evaluate genetic resources of endophytes, we analyzed the biodiversity of fungal microbiome of black crowberry (Empetrum nigrum L.) by next-generation sequencing and found that it consists mainly of unknown taxa. We then separated the host and the endophyte genomes and constructed a fosmid expression library from the endophytic DNA. This library was screened for antibacterial activity against Staphylococcus aureus. A unique antibacterial clone was selected for further analysis, and a gene En-AP1 was identified with no similarity to known sequences. The expressed, folded protein En-AP1 was not active against S. aureus, while tryptic digests exhibited antimicrobial activity. Seven out of twelve synthesized peptides, predicted antibacterial in silico, exhibited in vitro activity towards both S. aureus and Escherichia coli. We propose that the En-AP1 protein is degraded in the library host E. coli and antimicrobial fragments are released from the cell, explaining the in vitro antibacterial activity of the clone. This is the first report of a novel gene expressed in vitro derived from an endophytic microbiome, demonstrating the potential of finding novel genes and compounds from unculturable endophytes. Article in Journal/Newspaper Crowberry Empetrum nigrum Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) Applied Microbiology and Biotechnology 100 21 9283 9293 |
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Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
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ftunitriestiris |
language |
English |
topic |
Antimicrobial peptide Endophyte Microbiome Unculturable Anti-Bacterial Agent Ericaceae Escherichia coli Fungi Genetic Testing Peptide Staphylococcus aureu Biotechnology Applied Microbiology and Biotechnology |
spellingShingle |
Antimicrobial peptide Endophyte Microbiome Unculturable Anti-Bacterial Agent Ericaceae Escherichia coli Fungi Genetic Testing Peptide Staphylococcus aureu Biotechnology Applied Microbiology and Biotechnology Tejesvi, M. V Picart, P. Kajula, M. Hautajärvi, H. Ruddock, L. Kristensen, H. H. Sahl, H. G. Ek, S. Mattila, S. Pirttilä, A. M. TOSSI, ALESSANDRO Identification of antibacterial peptides from endophytic microbiome |
topic_facet |
Antimicrobial peptide Endophyte Microbiome Unculturable Anti-Bacterial Agent Ericaceae Escherichia coli Fungi Genetic Testing Peptide Staphylococcus aureu Biotechnology Applied Microbiology and Biotechnology |
description |
Endophytes, microorganisms living inside plant tissues, are promising producers of lead compounds for the pharmaceutical industry. However, the majority of endophytes are unculturable and therefore inaccessible for functional studies. To evaluate genetic resources of endophytes, we analyzed the biodiversity of fungal microbiome of black crowberry (Empetrum nigrum L.) by next-generation sequencing and found that it consists mainly of unknown taxa. We then separated the host and the endophyte genomes and constructed a fosmid expression library from the endophytic DNA. This library was screened for antibacterial activity against Staphylococcus aureus. A unique antibacterial clone was selected for further analysis, and a gene En-AP1 was identified with no similarity to known sequences. The expressed, folded protein En-AP1 was not active against S. aureus, while tryptic digests exhibited antimicrobial activity. Seven out of twelve synthesized peptides, predicted antibacterial in silico, exhibited in vitro activity towards both S. aureus and Escherichia coli. We propose that the En-AP1 protein is degraded in the library host E. coli and antimicrobial fragments are released from the cell, explaining the in vitro antibacterial activity of the clone. This is the first report of a novel gene expressed in vitro derived from an endophytic microbiome, demonstrating the potential of finding novel genes and compounds from unculturable endophytes. |
author2 |
Tejesvi, M. V Picart, P. Kajula, M. Hautajärvi, H. Ruddock, L. Kristensen, H. H. Tossi, Alessandro Sahl, H. G. Ek, S. Mattila, S. Pirttilä, A. M. |
format |
Article in Journal/Newspaper |
author |
Tejesvi, M. V Picart, P. Kajula, M. Hautajärvi, H. Ruddock, L. Kristensen, H. H. Sahl, H. G. Ek, S. Mattila, S. Pirttilä, A. M. TOSSI, ALESSANDRO |
author_facet |
Tejesvi, M. V Picart, P. Kajula, M. Hautajärvi, H. Ruddock, L. Kristensen, H. H. Sahl, H. G. Ek, S. Mattila, S. Pirttilä, A. M. TOSSI, ALESSANDRO |
author_sort |
Tejesvi, M. V |
title |
Identification of antibacterial peptides from endophytic microbiome |
title_short |
Identification of antibacterial peptides from endophytic microbiome |
title_full |
Identification of antibacterial peptides from endophytic microbiome |
title_fullStr |
Identification of antibacterial peptides from endophytic microbiome |
title_full_unstemmed |
Identification of antibacterial peptides from endophytic microbiome |
title_sort |
identification of antibacterial peptides from endophytic microbiome |
publishDate |
2016 |
url |
http://hdl.handle.net/11368/2906611 https://doi.org/10.1007/s00253-016-7765-4 https://link.springer.com/article/10.1007%2Fs00253-016-7765-4 |
genre |
Crowberry Empetrum nigrum |
genre_facet |
Crowberry Empetrum nigrum |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/27541748 info:eu-repo/semantics/altIdentifier/wos/WOS:000385129600024 volume:100 issue:21 firstpage:9283 lastpage:9293 numberofpages:11 journal:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY http://hdl.handle.net/11368/2906611 doi:10.1007/s00253-016-7765-4 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84982273161 https://link.springer.com/article/10.1007%2Fs00253-016-7765-4 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s00253-016-7765-4 |
container_title |
Applied Microbiology and Biotechnology |
container_volume |
100 |
container_issue |
21 |
container_start_page |
9283 |
op_container_end_page |
9293 |
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1766395420396748800 |