Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach
The present study addresses comprehensively the problem of producing polyesters through sustainable processes while using fully renewable raw materials and biocatalysts. Polycondensation of bio-based dimethyl adipate with different diols was catalyzed by cutinase 1 from Thermobifida cellulosilytica...
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Online Access: | http://hdl.handle.net/11368/2886133 https://doi.org/10.1039/C6GC02142E http://pubs.rsc.org/en/content/articlelanding/2017/gc/c6gc02142e#!divAbstract |
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ftunitriestiris:oai:arts.units.it:11368/2886133 2023-05-15T14:13:42+02:00 Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach Pellis, Alessandro FERRARIO, VALERIO Cespugli, Marco Corici, Livia Guarneri, Alice Zartl, Barbara Herrero Acero, Enrique EBERT, CYNTHIA Guebitz, Georg M. GARDOSSI, Lucia Pellis, Alessandro Ferrario, Valerio Cespugli, Marco Corici, Livia Guarneri, Alice Zartl, Barbara Herrero Acero, Enrique Ebert, Cynthia Guebitz, Georg M. Gardossi, Lucia 2017 ELETTRONICO http://hdl.handle.net/11368/2886133 https://doi.org/10.1039/C6GC02142E http://pubs.rsc.org/en/content/articlelanding/2017/gc/c6gc02142e#!divAbstract eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000393686400021 volume:19 issue:2 firstpage:490 lastpage:502 numberofpages:13 journal:GREEN CHEMISTRY info:eu-repo/grantAgreement/EC/FP7/REFINE project; no. 289253 http://hdl.handle.net/11368/2886133 doi:10.1039/C6GC02142E info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85008199094 http://pubs.rsc.org/en/content/articlelanding/2017/gc/c6gc02142e#!divAbstract info:eu-repo/semantics/openAccess polyester bio-based renewable biocatalysi cutinase enzyme immobilization rice husk design of experiments info:eu-repo/semantics/article 2017 ftunitriestiris https://doi.org/10.1039/C6GC02142E 2023-04-09T06:13:43Z The present study addresses comprehensively the problem of producing polyesters through sustainable processes while using fully renewable raw materials and biocatalysts. Polycondensation of bio-based dimethyl adipate with different diols was catalyzed by cutinase 1 from Thermobifida cellulosilytica (Thc_cut1) under solvent free and thin-film conditions. The biocatalyst was immobilized efficiently on a fully renewable cheap carrier based on milled rice husk. A multivariate factorial design demonstrated that Thc_cut1 is less sensitive to the presence of water in the system and it works efficiently under milder conditions (50 °C; 535 mbar) when compared to lipase B from Candida antarctica (CaLB), thus enabling energy savings. Experimental and computational investigations of cutinase 1 from Thermobifida cellulosilytica (Thc_cut1) disclosed structural and functional features that make this serine-hydrolase efficient in polycondensation reactions. Bioinformatic analysis performed with the BioGPS tool pointed out functional similarities with CaLB and provided guidelines for future engineering studies aiming, for instance, at introducing different promiscuous activities in the Thc_cut1 scaffold. The results set robust premises for a full exploitation of enzymes in environmentally and economically sustainable enzymatic polycondensation reactions. Article in Journal/Newspaper Antarc* Antarctica Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) Green Chemistry 19 2 490 502 |
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Open Polar |
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Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
op_collection_id |
ftunitriestiris |
language |
English |
topic |
polyester bio-based renewable biocatalysi cutinase enzyme immobilization rice husk design of experiments |
spellingShingle |
polyester bio-based renewable biocatalysi cutinase enzyme immobilization rice husk design of experiments Pellis, Alessandro FERRARIO, VALERIO Cespugli, Marco Corici, Livia Guarneri, Alice Zartl, Barbara Herrero Acero, Enrique EBERT, CYNTHIA Guebitz, Georg M. GARDOSSI, Lucia Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach |
topic_facet |
polyester bio-based renewable biocatalysi cutinase enzyme immobilization rice husk design of experiments |
description |
The present study addresses comprehensively the problem of producing polyesters through sustainable processes while using fully renewable raw materials and biocatalysts. Polycondensation of bio-based dimethyl adipate with different diols was catalyzed by cutinase 1 from Thermobifida cellulosilytica (Thc_cut1) under solvent free and thin-film conditions. The biocatalyst was immobilized efficiently on a fully renewable cheap carrier based on milled rice husk. A multivariate factorial design demonstrated that Thc_cut1 is less sensitive to the presence of water in the system and it works efficiently under milder conditions (50 °C; 535 mbar) when compared to lipase B from Candida antarctica (CaLB), thus enabling energy savings. Experimental and computational investigations of cutinase 1 from Thermobifida cellulosilytica (Thc_cut1) disclosed structural and functional features that make this serine-hydrolase efficient in polycondensation reactions. Bioinformatic analysis performed with the BioGPS tool pointed out functional similarities with CaLB and provided guidelines for future engineering studies aiming, for instance, at introducing different promiscuous activities in the Thc_cut1 scaffold. The results set robust premises for a full exploitation of enzymes in environmentally and economically sustainable enzymatic polycondensation reactions. |
author2 |
Pellis, Alessandro Ferrario, Valerio Cespugli, Marco Corici, Livia Guarneri, Alice Zartl, Barbara Herrero Acero, Enrique Ebert, Cynthia Guebitz, Georg M. Gardossi, Lucia |
format |
Article in Journal/Newspaper |
author |
Pellis, Alessandro FERRARIO, VALERIO Cespugli, Marco Corici, Livia Guarneri, Alice Zartl, Barbara Herrero Acero, Enrique EBERT, CYNTHIA Guebitz, Georg M. GARDOSSI, Lucia |
author_facet |
Pellis, Alessandro FERRARIO, VALERIO Cespugli, Marco Corici, Livia Guarneri, Alice Zartl, Barbara Herrero Acero, Enrique EBERT, CYNTHIA Guebitz, Georg M. GARDOSSI, Lucia |
author_sort |
Pellis, Alessandro |
title |
Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach |
title_short |
Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach |
title_full |
Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach |
title_fullStr |
Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach |
title_full_unstemmed |
Fully renewable polyesters via polycondensation catalyzed by Thermobifida cellulosilytica cutinase 1: an integrated approach |
title_sort |
fully renewable polyesters via polycondensation catalyzed by thermobifida cellulosilytica cutinase 1: an integrated approach |
publishDate |
2017 |
url |
http://hdl.handle.net/11368/2886133 https://doi.org/10.1039/C6GC02142E http://pubs.rsc.org/en/content/articlelanding/2017/gc/c6gc02142e#!divAbstract |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000393686400021 volume:19 issue:2 firstpage:490 lastpage:502 numberofpages:13 journal:GREEN CHEMISTRY info:eu-repo/grantAgreement/EC/FP7/REFINE project; no. 289253 http://hdl.handle.net/11368/2886133 doi:10.1039/C6GC02142E info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85008199094 http://pubs.rsc.org/en/content/articlelanding/2017/gc/c6gc02142e#!divAbstract |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1039/C6GC02142E |
container_title |
Green Chemistry |
container_volume |
19 |
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2 |
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490 |
op_container_end_page |
502 |
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1766286187487559680 |