IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses
The recent discovery of soluble immune-regulatory molecules in invertebrates takes advantage of the rapid growth of next generation sequencing datasets. Following protein domain searches in the transcriptomes of 31 bivalve spp. and in few available mollusk genomes, we retrieved 59 domains uniquely i...
Published in: | Developmental & Comparative Immunology |
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ftunitriestiris:oai:arts.units.it:11368/2845712 2023-05-15T15:58:54+02:00 IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses Rosani, Umberto Varotto, Laura GERDOL, MARCO PALLAVICINI, Alberto VENIER, PAOLA Rosani, Umberto Varotto, Laura Gerdol, Marco Pallavicini, Alberto Venier, Paola 2015 STAMPA http://hdl.handle.net/11368/2845712 https://doi.org/10.1016/j.dci.2015.05.001 eng eng info:eu-repo/semantics/altIdentifier/pmid/26026244 info:eu-repo/semantics/altIdentifier/wos/WOS:000360592200016 volume:52 issue:2 firstpage:255 lastpage:268 numberofpages:14 journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://hdl.handle.net/11368/2845712 doi:10.1016/j.dci.2015.05.001 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84934914410 info:eu-repo/semantics/openAccess Bivalve Crassostrea giga Innate immunity Interleukin 17 Mytilus galloprovincialis info:eu-repo/semantics/article 2015 ftunitriestiris https://doi.org/10.1016/j.dci.2015.05.001 2023-04-09T06:14:31Z The recent discovery of soluble immune-regulatory molecules in invertebrates takes advantage of the rapid growth of next generation sequencing datasets. Following protein domain searches in the transcriptomes of 31 bivalve spp. and in few available mollusk genomes, we retrieved 59 domains uniquely identifying interleukin 17 (IL-17) and 96 SEFIR domains typical of IL-17 receptors and CIKS/ACT1 proteins acting downstream in the IL-17 signaling pathway. Compared to the Chordata IL-17 family members, we confirm a separate clustering of the bivalve domain sequences and a consistent conservation pattern of amino acid residues. Analysis performed at transcript and genome level allowed us to propose an updated view of the components outlining the IL-17 signaling pathway in Mytilus galloprovincialis and Crassostrea gigas (in both species, homology modeling reduced the variety of IL-17 domains to only two 3D structures). Digital expression analysis indicated more heterogeneous expression levels for the mussel and oyster IL-17 ligands than for IL-17 receptors and CIKS/CIKSL proteins. Besides, new qPCR analyses confirmed such gene expression trends in hemocytes and gills of mussels challenged with heat-killed bacteria. These results uphold the involvement of an ancient IL-17 signaling pathway in the bivalve immune responses and, likewise in humans, suggest the possibility of distinctive modulatory roles of individual IL-17s/IL-17 receptors. Overall, the common evidence of pro-inflammatory cytokines and inter-related intracellular signaling pathways in bivalves definitely adds complexity to the invertebrate immunity. Article in Journal/Newspaper Crassostrea gigas Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) Developmental & Comparative Immunology 52 2 255 268 |
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Open Polar |
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Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
op_collection_id |
ftunitriestiris |
language |
English |
topic |
Bivalve Crassostrea giga Innate immunity Interleukin 17 Mytilus galloprovincialis |
spellingShingle |
Bivalve Crassostrea giga Innate immunity Interleukin 17 Mytilus galloprovincialis Rosani, Umberto Varotto, Laura GERDOL, MARCO PALLAVICINI, Alberto VENIER, PAOLA IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses |
topic_facet |
Bivalve Crassostrea giga Innate immunity Interleukin 17 Mytilus galloprovincialis |
description |
The recent discovery of soluble immune-regulatory molecules in invertebrates takes advantage of the rapid growth of next generation sequencing datasets. Following protein domain searches in the transcriptomes of 31 bivalve spp. and in few available mollusk genomes, we retrieved 59 domains uniquely identifying interleukin 17 (IL-17) and 96 SEFIR domains typical of IL-17 receptors and CIKS/ACT1 proteins acting downstream in the IL-17 signaling pathway. Compared to the Chordata IL-17 family members, we confirm a separate clustering of the bivalve domain sequences and a consistent conservation pattern of amino acid residues. Analysis performed at transcript and genome level allowed us to propose an updated view of the components outlining the IL-17 signaling pathway in Mytilus galloprovincialis and Crassostrea gigas (in both species, homology modeling reduced the variety of IL-17 domains to only two 3D structures). Digital expression analysis indicated more heterogeneous expression levels for the mussel and oyster IL-17 ligands than for IL-17 receptors and CIKS/CIKSL proteins. Besides, new qPCR analyses confirmed such gene expression trends in hemocytes and gills of mussels challenged with heat-killed bacteria. These results uphold the involvement of an ancient IL-17 signaling pathway in the bivalve immune responses and, likewise in humans, suggest the possibility of distinctive modulatory roles of individual IL-17s/IL-17 receptors. Overall, the common evidence of pro-inflammatory cytokines and inter-related intracellular signaling pathways in bivalves definitely adds complexity to the invertebrate immunity. |
author2 |
Rosani, Umberto Varotto, Laura Gerdol, Marco Pallavicini, Alberto Venier, Paola |
format |
Article in Journal/Newspaper |
author |
Rosani, Umberto Varotto, Laura GERDOL, MARCO PALLAVICINI, Alberto VENIER, PAOLA |
author_facet |
Rosani, Umberto Varotto, Laura GERDOL, MARCO PALLAVICINI, Alberto VENIER, PAOLA |
author_sort |
Rosani, Umberto |
title |
IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses |
title_short |
IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses |
title_full |
IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses |
title_fullStr |
IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses |
title_full_unstemmed |
IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses |
title_sort |
il-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses |
publishDate |
2015 |
url |
http://hdl.handle.net/11368/2845712 https://doi.org/10.1016/j.dci.2015.05.001 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/26026244 info:eu-repo/semantics/altIdentifier/wos/WOS:000360592200016 volume:52 issue:2 firstpage:255 lastpage:268 numberofpages:14 journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://hdl.handle.net/11368/2845712 doi:10.1016/j.dci.2015.05.001 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84934914410 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.dci.2015.05.001 |
container_title |
Developmental & Comparative Immunology |
container_volume |
52 |
container_issue |
2 |
container_start_page |
255 |
op_container_end_page |
268 |
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1766394677302394880 |