Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis
Based on protein domain structure and organization deduced from mRNA contigs, 15 transcripts of the Toll signaling pathway have been identified in the bivalve, Mytilus galloprovincialis. Identical searches performed on publicly available Mytilus edulis ESTs revealed 11 transcripts, whereas searches...
Published in: | Developmental & Comparative Immunology |
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Online Access: | http://hdl.handle.net/11368/2784139 https://doi.org/10.1016/j.dci.2014.03.021 http://www.sciencedirect.com/science/article/pii/S0145305X14000925 |
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ftunitriestiris:oai:arts.units.it:11368/2784139 2023-05-15T15:58:43+02:00 Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis Mylène Toubiana Umberto Rosani Sonia Giambelluca Matteo Cammarata Paola Venier Philippe Roch GERDOL, MARCO PALLAVICINI, Alberto Mylène, Toubiana Umberto, Rosani Sonia, Giambelluca Matteo, Cammarata Gerdol, Marco Pallavicini, Alberto Paola, Venier Philippe, Roch 2014 STAMPA http://hdl.handle.net/11368/2784139 https://doi.org/10.1016/j.dci.2014.03.021 http://www.sciencedirect.com/science/article/pii/S0145305X14000925 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000337856600014 volume:45 firstpage:300 lastpage:312 numberofpages:13 journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://hdl.handle.net/11368/2784139 doi:10.1016/j.dci.2014.03.021 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84899133661 http://www.sciencedirect.com/science/article/pii/S0145305X14000925 Innate immunity Mollusk Mytilu Signal transduction Toll pathway NF-κB info:eu-repo/semantics/article 2014 ftunitriestiris https://doi.org/10.1016/j.dci.2014.03.021 2023-04-09T06:11:45Z Based on protein domain structure and organization deduced from mRNA contigs, 15 transcripts of the Toll signaling pathway have been identified in the bivalve, Mytilus galloprovincialis. Identical searches performed on publicly available Mytilus edulis ESTs revealed 11 transcripts, whereas searches performed in genomic and new transcriptome sequences of the Pacific oyster, Crassostrea gigas, identified 21 Toll-related transcripts. The remarkable molecular diversity of TRAF and IKK coding sequences of C. gigas, suggests that the sequence data inferred from Mytilus cDNAs may not be exhaustive. Most of the Toll pathway genes were constitutively and ubiquitously expressed in M. galloprovincialis, although at different levels, and clearly induced after in vivo injection with bacteria. Such over-transcription was more rapid and intense with Gram-negative than with Gram-positive bacteria. Injection of a fungus modulated the transcription of few Toll pathway genes, with the induction levels of TLR/MyD88 complex being always less intense. Purified LPS and β-glucans had marginal effect whereas peptidoglycans were ineffective. At the moment, we found no evidence of an IMD transcript in bivalves. In conclusion, mussels possess a complete Toll pathway which can be triggered either by Gram-positive or Gram-negative bacteria Article in Journal/Newspaper Crassostrea gigas Pacific oyster Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) Pacific Developmental & Comparative Immunology 45 2 300 312 |
institution |
Open Polar |
collection |
Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
op_collection_id |
ftunitriestiris |
language |
English |
topic |
Innate immunity Mollusk Mytilu Signal transduction Toll pathway NF-κB |
spellingShingle |
Innate immunity Mollusk Mytilu Signal transduction Toll pathway NF-κB Mylène Toubiana Umberto Rosani Sonia Giambelluca Matteo Cammarata Paola Venier Philippe Roch GERDOL, MARCO PALLAVICINI, Alberto Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis |
topic_facet |
Innate immunity Mollusk Mytilu Signal transduction Toll pathway NF-κB |
description |
Based on protein domain structure and organization deduced from mRNA contigs, 15 transcripts of the Toll signaling pathway have been identified in the bivalve, Mytilus galloprovincialis. Identical searches performed on publicly available Mytilus edulis ESTs revealed 11 transcripts, whereas searches performed in genomic and new transcriptome sequences of the Pacific oyster, Crassostrea gigas, identified 21 Toll-related transcripts. The remarkable molecular diversity of TRAF and IKK coding sequences of C. gigas, suggests that the sequence data inferred from Mytilus cDNAs may not be exhaustive. Most of the Toll pathway genes were constitutively and ubiquitously expressed in M. galloprovincialis, although at different levels, and clearly induced after in vivo injection with bacteria. Such over-transcription was more rapid and intense with Gram-negative than with Gram-positive bacteria. Injection of a fungus modulated the transcription of few Toll pathway genes, with the induction levels of TLR/MyD88 complex being always less intense. Purified LPS and β-glucans had marginal effect whereas peptidoglycans were ineffective. At the moment, we found no evidence of an IMD transcript in bivalves. In conclusion, mussels possess a complete Toll pathway which can be triggered either by Gram-positive or Gram-negative bacteria |
author2 |
Mylène, Toubiana Umberto, Rosani Sonia, Giambelluca Matteo, Cammarata Gerdol, Marco Pallavicini, Alberto Paola, Venier Philippe, Roch |
format |
Article in Journal/Newspaper |
author |
Mylène Toubiana Umberto Rosani Sonia Giambelluca Matteo Cammarata Paola Venier Philippe Roch GERDOL, MARCO PALLAVICINI, Alberto |
author_facet |
Mylène Toubiana Umberto Rosani Sonia Giambelluca Matteo Cammarata Paola Venier Philippe Roch GERDOL, MARCO PALLAVICINI, Alberto |
author_sort |
Mylène Toubiana |
title |
Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis |
title_short |
Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis |
title_full |
Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis |
title_fullStr |
Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis |
title_full_unstemmed |
Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis |
title_sort |
toll signal transduction pathway in bivalves: complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated mytilus galloprovincialis |
publishDate |
2014 |
url |
http://hdl.handle.net/11368/2784139 https://doi.org/10.1016/j.dci.2014.03.021 http://www.sciencedirect.com/science/article/pii/S0145305X14000925 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000337856600014 volume:45 firstpage:300 lastpage:312 numberofpages:13 journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://hdl.handle.net/11368/2784139 doi:10.1016/j.dci.2014.03.021 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84899133661 http://www.sciencedirect.com/science/article/pii/S0145305X14000925 |
op_doi |
https://doi.org/10.1016/j.dci.2014.03.021 |
container_title |
Developmental & Comparative Immunology |
container_volume |
45 |
container_issue |
2 |
container_start_page |
300 |
op_container_end_page |
312 |
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1766394496529989632 |