Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in
Previous studies described high concentrations of mercury (Hg) and selenium(Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferati...
Published in: | Marine Pollution Bulletin |
---|---|
Main Authors: | , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier Ltd
2016
|
Subjects: | |
Online Access: | http://hdl.handle.net/2078.1/174919 https://doi.org/10.1016/j.marpolbul.2016.04.060 |
id |
ftunistlouisbrus:oai:dial.uclouvain.be:boreal:174919 |
---|---|
record_format |
openpolar |
spelling |
ftunistlouisbrus:oai:dial.uclouvain.be:boreal:174919 2024-05-12T08:04:52+00:00 Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in Das, Krishna Dupont, Aurélie De Pauw-Gillet, Marie-Claire Debier, Cathy Siebert, Ursula UCL - SST/ISV - Institut des sciences de la vie 2016 http://hdl.handle.net/2078.1/174919 https://doi.org/10.1016/j.marpolbul.2016.04.060 eng eng Elsevier Ltd boreal:174919 http://hdl.handle.net/2078.1/174919 doi:10.1016/j.marpolbul.2016.04.060 urn:ISSN:0025-326X urn:EISSN:1879-3363 info:eu-repo/semantics/openAccess Marine Pollution Bulletin, Vol. 108, p. 70-76 (may 2016) info:eu-repo/semantics/article 2016 ftunistlouisbrus https://doi.org/10.1016/j.marpolbul.2016.04.060 2024-04-18T17:41:23Z Previous studies described high concentrations of mercury (Hg) and selenium(Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 μM. In vitro exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 μM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential. To conclude, our results clearly demonstrated that MeHg affected in vitro immune cells exposurewith no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea. Article in Journal/Newspaper harbour seal Phoca vitulina DIAL@USL-B (Université Saint-Louis, Bruxelles) Marine Pollution Bulletin 108 1-2 70 76 |
institution |
Open Polar |
collection |
DIAL@USL-B (Université Saint-Louis, Bruxelles) |
op_collection_id |
ftunistlouisbrus |
language |
English |
description |
Previous studies described high concentrations of mercury (Hg) and selenium(Se) in the blood of harbour seals, Phoca vitulina from the North Sea. In the present study, we evaluated the in vitro potential protective effects of sodium selenite (Na2SeO3) and selenomethionine (SeMet) on cell proliferation of harbour seal lymphocytes exposed to MeHgCl 0.75 μM. In vitro exposure of ConA-stimulated T lymphocytes resulted in severe inhibition of DNA synthesis, likely linked to severe loss of mitochondrial membrane potential at 0.75 μM. Neither selenite nor SeMet showed a protective effect against MeHg toxicity expressed at the T lymphocyte proliferation level for harbour seals. Selenite and SeMet did not show negative effects regarding lymphocyte proliferation and mitochondrial membrane potential. To conclude, our results clearly demonstrated that MeHg affected in vitro immune cells exposurewith no protective effects of selenium at a molar ratio Hg:Se of 1:10 in harbour seals from the North Sea. |
author2 |
UCL - SST/ISV - Institut des sciences de la vie |
format |
Article in Journal/Newspaper |
author |
Das, Krishna Dupont, Aurélie De Pauw-Gillet, Marie-Claire Debier, Cathy Siebert, Ursula |
spellingShingle |
Das, Krishna Dupont, Aurélie De Pauw-Gillet, Marie-Claire Debier, Cathy Siebert, Ursula Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in |
author_facet |
Das, Krishna Dupont, Aurélie De Pauw-Gillet, Marie-Claire Debier, Cathy Siebert, Ursula |
author_sort |
Das, Krishna |
title |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in |
title_short |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in |
title_full |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in |
title_fullStr |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in |
title_full_unstemmed |
Absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in |
title_sort |
absence of selenium protection against methylmercury toxicity in harbour seal leucocytes in |
publisher |
Elsevier Ltd |
publishDate |
2016 |
url |
http://hdl.handle.net/2078.1/174919 https://doi.org/10.1016/j.marpolbul.2016.04.060 |
genre |
harbour seal Phoca vitulina |
genre_facet |
harbour seal Phoca vitulina |
op_source |
Marine Pollution Bulletin, Vol. 108, p. 70-76 (may 2016) |
op_relation |
boreal:174919 http://hdl.handle.net/2078.1/174919 doi:10.1016/j.marpolbul.2016.04.060 urn:ISSN:0025-326X urn:EISSN:1879-3363 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.marpolbul.2016.04.060 |
container_title |
Marine Pollution Bulletin |
container_volume |
108 |
container_issue |
1-2 |
container_start_page |
70 |
op_container_end_page |
76 |
_version_ |
1798847130494828544 |