Site-specific Counterion Binding - Application of the Standard Poisson-boltzmann Cell Model To Ionic Polysaccharides of the Plant-cell Wall
A numerical method is presented for analysing the effects of the competitive processes of dissociation and complexation that occur at the level of the functional groups of charged polysaccharides during ion exchange experiments carried out on plant cell walls. The interactions between the exchange s...
Published in: | Biophysical Chemistry |
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Online Access: | http://hdl.handle.net/2078.2/101920 https://doi.org/10.1016/0301-4622(94)00003-4 |
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ftunistlouisbrus:oai:dial.uclouvain.be:boreal:101920 2023-05-15T17:23:50+02:00 Site-specific Counterion Binding - Application of the Standard Poisson-boltzmann Cell Model To Ionic Polysaccharides of the Plant-cell Wall Voue, M. Gillet, C. FUNDP 1994 http://hdl.handle.net/2078.2/101920 https://doi.org/10.1016/0301-4622(94)00003-4 eng eng Elsevier Science Bv boreal:101920 http://hdl.handle.net/2078.2/101920 doi:10.1016/0301-4622(94)00003-4 urn:ISSN:0301-4622 urn:EISSN:1873-4200 Biophysical Chemistry, Vol. 51, no. 1, p. 9-19 (1994) PLANT CELL WALL POISSON-BOLTZMANN CELL MODEL COUNTERION BINDING POLYGALACTURONIC ACID NITELLA FLEXILIS info:eu-repo/semantics/article 1994 ftunistlouisbrus https://doi.org/10.1016/0301-4622(94)00003-4 2018-05-16T22:55:57Z A numerical method is presented for analysing the effects of the competitive processes of dissociation and complexation that occur at the level of the functional groups of charged polysaccharides during ion exchange experiments carried out on plant cell walls. The interactions between the exchange sites and the mobile ions are handled by the standard Poisson-Boltzmann cell model. The dissociation and the complexation are described by mass-action laws. Uncomplexed counterions are involved in the formation of a cylindrical double layer. This latter type of interaction determines the dissociation degree of the polyion. A least-squares algorithm is used to evaluate the complexation constants of the counterions from experimental data. The need of introduction of the complexation constant of the divalent counterions has been clarified by a comparison between theoretical titration curves calculated with and without specific site binding. The fraction of complexed sites does not agree with that predicted from Oosawa's theory of the condensation. The accumulation coefficients, calculated from the optimised theoretical titration curves, clearly demonstrate the competition between the protonation and the complexation processes at the level of the pectic acids of the wall. Article in Journal/Newspaper Nitella flexilis DIAL@USL-B (Université Saint-Louis, Bruxelles) Biophysical Chemistry 51 1 9 19 |
institution |
Open Polar |
collection |
DIAL@USL-B (Université Saint-Louis, Bruxelles) |
op_collection_id |
ftunistlouisbrus |
language |
English |
topic |
PLANT CELL WALL POISSON-BOLTZMANN CELL MODEL COUNTERION BINDING POLYGALACTURONIC ACID NITELLA FLEXILIS |
spellingShingle |
PLANT CELL WALL POISSON-BOLTZMANN CELL MODEL COUNTERION BINDING POLYGALACTURONIC ACID NITELLA FLEXILIS Voue, M. Gillet, C. Site-specific Counterion Binding - Application of the Standard Poisson-boltzmann Cell Model To Ionic Polysaccharides of the Plant-cell Wall |
topic_facet |
PLANT CELL WALL POISSON-BOLTZMANN CELL MODEL COUNTERION BINDING POLYGALACTURONIC ACID NITELLA FLEXILIS |
description |
A numerical method is presented for analysing the effects of the competitive processes of dissociation and complexation that occur at the level of the functional groups of charged polysaccharides during ion exchange experiments carried out on plant cell walls. The interactions between the exchange sites and the mobile ions are handled by the standard Poisson-Boltzmann cell model. The dissociation and the complexation are described by mass-action laws. Uncomplexed counterions are involved in the formation of a cylindrical double layer. This latter type of interaction determines the dissociation degree of the polyion. A least-squares algorithm is used to evaluate the complexation constants of the counterions from experimental data. The need of introduction of the complexation constant of the divalent counterions has been clarified by a comparison between theoretical titration curves calculated with and without specific site binding. The fraction of complexed sites does not agree with that predicted from Oosawa's theory of the condensation. The accumulation coefficients, calculated from the optimised theoretical titration curves, clearly demonstrate the competition between the protonation and the complexation processes at the level of the pectic acids of the wall. |
author2 |
FUNDP |
format |
Article in Journal/Newspaper |
author |
Voue, M. Gillet, C. |
author_facet |
Voue, M. Gillet, C. |
author_sort |
Voue, M. |
title |
Site-specific Counterion Binding - Application of the Standard Poisson-boltzmann Cell Model To Ionic Polysaccharides of the Plant-cell Wall |
title_short |
Site-specific Counterion Binding - Application of the Standard Poisson-boltzmann Cell Model To Ionic Polysaccharides of the Plant-cell Wall |
title_full |
Site-specific Counterion Binding - Application of the Standard Poisson-boltzmann Cell Model To Ionic Polysaccharides of the Plant-cell Wall |
title_fullStr |
Site-specific Counterion Binding - Application of the Standard Poisson-boltzmann Cell Model To Ionic Polysaccharides of the Plant-cell Wall |
title_full_unstemmed |
Site-specific Counterion Binding - Application of the Standard Poisson-boltzmann Cell Model To Ionic Polysaccharides of the Plant-cell Wall |
title_sort |
site-specific counterion binding - application of the standard poisson-boltzmann cell model to ionic polysaccharides of the plant-cell wall |
publisher |
Elsevier Science Bv |
publishDate |
1994 |
url |
http://hdl.handle.net/2078.2/101920 https://doi.org/10.1016/0301-4622(94)00003-4 |
genre |
Nitella flexilis |
genre_facet |
Nitella flexilis |
op_source |
Biophysical Chemistry, Vol. 51, no. 1, p. 9-19 (1994) |
op_relation |
boreal:101920 http://hdl.handle.net/2078.2/101920 doi:10.1016/0301-4622(94)00003-4 urn:ISSN:0301-4622 urn:EISSN:1873-4200 |
op_doi |
https://doi.org/10.1016/0301-4622(94)00003-4 |
container_title |
Biophysical Chemistry |
container_volume |
51 |
container_issue |
1 |
container_start_page |
9 |
op_container_end_page |
19 |
_version_ |
1766114285459603456 |