Photothermal Studies of Carboxymyoglobin

Small ligand diffusion in heme proteins is not fully understood. To help better understand CO diffusion, three systems were investigated: L29H/F43H site-directed sperm whale myoglobin, horse heart myoglobin in a heavy water buffer, and calix[4]resorcinarene. Binding of copper to calix[4]resorcinaren...

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Main Author: Small, Meagan
Format: Thesis
Language:unknown
Published: Digital Commons @ University of South Florida 2010
Subjects:
Online Access:https://digitalcommons.usf.edu/etd/1775
https://digitalcommons.usf.edu/context/etd/article/2774/viewcontent/.pdf
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author Small, Meagan
author_facet Small, Meagan
author_sort Small, Meagan
collection Digital Commons University of South Florida (USF)
description Small ligand diffusion in heme proteins is not fully understood. To help better understand CO diffusion, three systems were investigated: L29H/F43H site-directed sperm whale myoglobin, horse heart myoglobin in a heavy water buffer, and calix[4]resorcinarene. Binding of copper to calix[4]resorcinarene was photophysically characterized to unravel transient binding of small molecules in heme-copper proteins. Copper binding was found to have a low dissociation constant of approximately 8.6 micrometers. Reaction profiles using photoacoustic calorimetry were constructed for the myoglobin systems. In deuterium oxide, ligand escape is not rate limited by water entry. Large enthalpy differences arise from the thermodynamic properties of deuterium oxide and the extensive hydrogen bonding network in myoglobin. In the mutant, CO rebinds primarily to the heme and is exothermic with a large volume contraction because of altered electrostatics within the binding pocket and higher water occupancy.
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spelling ftunisfloridatam:oai:digitalcommons.usf.edu:etd-2774 2025-01-17T00:58:10+00:00 Photothermal Studies of Carboxymyoglobin Small, Meagan 2010-07-15T07:00:00Z application/pdf https://digitalcommons.usf.edu/etd/1775 https://digitalcommons.usf.edu/context/etd/article/2774/viewcontent/.pdf unknown Digital Commons @ University of South Florida https://digitalcommons.usf.edu/etd/1775 https://digitalcommons.usf.edu/context/etd/article/2774/viewcontent/.pdf default USF Tampa Graduate Theses and Dissertations myoglobin thermodynamics heme proteins calixarenes photoacoustic calorimetry American Studies Arts and Humanities thesis 2010 ftunisfloridatam 2023-05-04T18:00:34Z Small ligand diffusion in heme proteins is not fully understood. To help better understand CO diffusion, three systems were investigated: L29H/F43H site-directed sperm whale myoglobin, horse heart myoglobin in a heavy water buffer, and calix[4]resorcinarene. Binding of copper to calix[4]resorcinarene was photophysically characterized to unravel transient binding of small molecules in heme-copper proteins. Copper binding was found to have a low dissociation constant of approximately 8.6 micrometers. Reaction profiles using photoacoustic calorimetry were constructed for the myoglobin systems. In deuterium oxide, ligand escape is not rate limited by water entry. Large enthalpy differences arise from the thermodynamic properties of deuterium oxide and the extensive hydrogen bonding network in myoglobin. In the mutant, CO rebinds primarily to the heme and is exothermic with a large volume contraction because of altered electrostatics within the binding pocket and higher water occupancy. Thesis Sperm whale Digital Commons University of South Florida (USF)
spellingShingle myoglobin
thermodynamics
heme proteins
calixarenes
photoacoustic calorimetry
American Studies
Arts and Humanities
Small, Meagan
Photothermal Studies of Carboxymyoglobin
title Photothermal Studies of Carboxymyoglobin
title_full Photothermal Studies of Carboxymyoglobin
title_fullStr Photothermal Studies of Carboxymyoglobin
title_full_unstemmed Photothermal Studies of Carboxymyoglobin
title_short Photothermal Studies of Carboxymyoglobin
title_sort photothermal studies of carboxymyoglobin
topic myoglobin
thermodynamics
heme proteins
calixarenes
photoacoustic calorimetry
American Studies
Arts and Humanities
topic_facet myoglobin
thermodynamics
heme proteins
calixarenes
photoacoustic calorimetry
American Studies
Arts and Humanities
url https://digitalcommons.usf.edu/etd/1775
https://digitalcommons.usf.edu/context/etd/article/2774/viewcontent/.pdf