LIPASES PRODUCED BY YEASTS: POWERFUL BIOCATALYSTS FOR INDUSTRIAL PURPOSES

The term “lipolytic enzymes” refers to the lipases and carboxylic ester hydrolases. Lipase production is widespread among yeasts, but few are capable of producing lipases with interesting characteristics and in sufficient amounts to be industrially useful. The literature concerning lipases produced...

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Bibliographic Details
Main Authors: Lock, Luiza Lux, Corbellini, Valeriano Antonio, Valente, Patrícia
Format: Article in Journal/Newspaper
Language:Portuguese
Published: Universidade de Santa Cruz do Sul 2007
Subjects:
microbiologia industrial
Candida antarctica
Candida rugosa
Lipase
Yarrowia lipolytica
levedura
Rugosa
Antarc*
Antarctica
Online Access:https://online.unisc.br/seer/index.php/tecnologica/article/view/153
https://doi.org/10.17058/tecnolog.v11i1.153
Description
Summary:The term “lipolytic enzymes” refers to the lipases and carboxylic ester hydrolases. Lipase production is widespread among yeasts, but few are capable of producing lipases with interesting characteristics and in sufficient amounts to be industrially useful. The literature concerning lipases produced by Candida rugosa, Yarrowia (Candida) lipolytica, Candida antarctica and other emerging lipase-producing yeasts is reviewed. The use of recombinant lipases is discussed, with emphasis on the utilization of heterologous expression systems and design of chimeras. Finally, the three approaches that aim the improvement of lipase production or the modification of the substrate selectivity of the enzyme (medium engineering, biocatalyst engineering, and protein engineering) are discussed. O termo “enzimas lipolíticas” refere-se a lípases e hidrolases éster carboxílico. A produção de lipase é ampla entre as leveduras, mas poucas são capazes de produzir lipases com características interessantes e em quantidades suficientes para serem industrialmente úteis. A literatura relativa a lipases produzidas por Candida rugosa, Yarrowia (Candida) lipolytica, Candida antarctica e outras leveduras produtoras de lípases é revisada. O uso de lípases recombinantes é discutido, com ênfase na utilização de sistemas de expressão heteróloga e desenho de quimeras. Finalmente, as três abordagens que visam à melhora da produção de lipase ou a modificação da seletividade do substrato da enzima (engenharia do meio, do biocatalisador e da proteína) são discutidos.

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