Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts
In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extr...
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Online Access: | http://hdl.handle.net/11386/4671515 https://doi.org/10.1016/j.chroma.2015.07.053 http://www.sciencedirect.com/science/article/pii/S0021967315010213 |
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ftunisalernoiris:oai:www.iris.unisa.it:11386/4671515 2024-02-11T10:06:17+01:00 Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts Montero, Lidia Sánchez Camargo, Andrea P. García Cañas, Virginia Tanniou, Anaëlle Stiger Pouvreau, Valérie Russo, Mariateresa RASTRELLI, Luca Cifuentes, Alejandro Herrero, Miguel Ibáñez, Elena Montero, Lidia Sánchez Camargo, Andrea P. García Cañas, Virginia Tanniou, Anaëlle Stiger Pouvreau, Valérie Russo, Mariateresa Rastrelli, Luca Cifuentes, Alejandro Herrero, Miguel Ibáñez, Elena 2016 http://hdl.handle.net/11386/4671515 https://doi.org/10.1016/j.chroma.2015.07.053 http://www.sciencedirect.com/science/article/pii/S0021967315010213 eng eng info:eu-repo/semantics/altIdentifier/pmid/26210109 info:eu-repo/semantics/altIdentifier/wos/WOS:000368564000009 volume:1428 firstpage:115 lastpage:125 numberofpages:11 journal:JOURNAL OF CHROMATOGRAPHY A http://hdl.handle.net/11386/4671515 doi:10.1016/j.chroma.2015.07.053 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84952982803 http://www.sciencedirect.com/science/article/pii/S0021967315010213 info:eu-repo/semantics/closedAccess Anti-proliferative activity Brown algae Comprehensive LC Phenolic compound Phlorotannin Sargassum muticum Antineoplastic Agent Atlantic Ocean Cell Proliferation HT29 Cell Human Phenol Phloroglucinol Sargassum Tannin Chromatography Liquid Tandem Mass Spectrometry Analytical Chemistry Organic Chemistry Biochemistry Medicine (all) info:eu-repo/semantics/article 2016 ftunisalernoiris https://doi.org/10.1016/j.chroma.2015.07.053 2024-01-24T17:42:50Z In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and Norway, were found to be the richest on total phenols and, particularly, on phlorotannins, containing up to 148.97 and 5.12mg phloroglucinol equivalents g-1, respectively. The extracts obtained from these locations were further purified and chemically characterized using a modified HILIC×RP-DAD-MS/MS method. The application of this methodology allowed the tentative identification of a great variability of phlorotannins with different degrees of polymerization (from 3 to 11) and structures, determined for the first time in S. muticum. The most-abundant phlorotannins on these samples were fuhalols, hydroxyfuhalols and phlorethols, showing also particularities and important differences depending on the geographical location. Afterwards, the antiproliferative activity of these extracts against HT-29 adenocarcinoma colon cancer cells was studied. Results revealed that the richest S. muticum samples in terms of total phlorotannins, i.e., those from Norway, presented the highest activity, showing a good cytotoxic potential at concentrations in the medium micromolar range. Article in Journal/Newspaper North Atlantic EleA@Unisa (Università degli Studi di Salerno) Norway Journal of Chromatography A 1428 115 125 |
institution |
Open Polar |
collection |
EleA@Unisa (Università degli Studi di Salerno) |
op_collection_id |
ftunisalernoiris |
language |
English |
topic |
Anti-proliferative activity Brown algae Comprehensive LC Phenolic compound Phlorotannin Sargassum muticum Antineoplastic Agent Atlantic Ocean Cell Proliferation HT29 Cell Human Phenol Phloroglucinol Sargassum Tannin Chromatography Liquid Tandem Mass Spectrometry Analytical Chemistry Organic Chemistry Biochemistry Medicine (all) |
spellingShingle |
Anti-proliferative activity Brown algae Comprehensive LC Phenolic compound Phlorotannin Sargassum muticum Antineoplastic Agent Atlantic Ocean Cell Proliferation HT29 Cell Human Phenol Phloroglucinol Sargassum Tannin Chromatography Liquid Tandem Mass Spectrometry Analytical Chemistry Organic Chemistry Biochemistry Medicine (all) Montero, Lidia Sánchez Camargo, Andrea P. García Cañas, Virginia Tanniou, Anaëlle Stiger Pouvreau, Valérie Russo, Mariateresa RASTRELLI, Luca Cifuentes, Alejandro Herrero, Miguel Ibáñez, Elena Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts |
topic_facet |
Anti-proliferative activity Brown algae Comprehensive LC Phenolic compound Phlorotannin Sargassum muticum Antineoplastic Agent Atlantic Ocean Cell Proliferation HT29 Cell Human Phenol Phloroglucinol Sargassum Tannin Chromatography Liquid Tandem Mass Spectrometry Analytical Chemistry Organic Chemistry Biochemistry Medicine (all) |
description |
In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and Norway, were found to be the richest on total phenols and, particularly, on phlorotannins, containing up to 148.97 and 5.12mg phloroglucinol equivalents g-1, respectively. The extracts obtained from these locations were further purified and chemically characterized using a modified HILIC×RP-DAD-MS/MS method. The application of this methodology allowed the tentative identification of a great variability of phlorotannins with different degrees of polymerization (from 3 to 11) and structures, determined for the first time in S. muticum. The most-abundant phlorotannins on these samples were fuhalols, hydroxyfuhalols and phlorethols, showing also particularities and important differences depending on the geographical location. Afterwards, the antiproliferative activity of these extracts against HT-29 adenocarcinoma colon cancer cells was studied. Results revealed that the richest S. muticum samples in terms of total phlorotannins, i.e., those from Norway, presented the highest activity, showing a good cytotoxic potential at concentrations in the medium micromolar range. |
author2 |
Montero, Lidia Sánchez Camargo, Andrea P. García Cañas, Virginia Tanniou, Anaëlle Stiger Pouvreau, Valérie Russo, Mariateresa Rastrelli, Luca Cifuentes, Alejandro Herrero, Miguel Ibáñez, Elena |
format |
Article in Journal/Newspaper |
author |
Montero, Lidia Sánchez Camargo, Andrea P. García Cañas, Virginia Tanniou, Anaëlle Stiger Pouvreau, Valérie Russo, Mariateresa RASTRELLI, Luca Cifuentes, Alejandro Herrero, Miguel Ibáñez, Elena |
author_facet |
Montero, Lidia Sánchez Camargo, Andrea P. García Cañas, Virginia Tanniou, Anaëlle Stiger Pouvreau, Valérie Russo, Mariateresa RASTRELLI, Luca Cifuentes, Alejandro Herrero, Miguel Ibáñez, Elena |
author_sort |
Montero, Lidia |
title |
Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts |
title_short |
Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts |
title_full |
Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts |
title_fullStr |
Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts |
title_full_unstemmed |
Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts |
title_sort |
anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga sargassum muticum collected on north-atlantic coasts |
publishDate |
2016 |
url |
http://hdl.handle.net/11386/4671515 https://doi.org/10.1016/j.chroma.2015.07.053 http://www.sciencedirect.com/science/article/pii/S0021967315010213 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/26210109 info:eu-repo/semantics/altIdentifier/wos/WOS:000368564000009 volume:1428 firstpage:115 lastpage:125 numberofpages:11 journal:JOURNAL OF CHROMATOGRAPHY A http://hdl.handle.net/11386/4671515 doi:10.1016/j.chroma.2015.07.053 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84952982803 http://www.sciencedirect.com/science/article/pii/S0021967315010213 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.chroma.2015.07.053 |
container_title |
Journal of Chromatography A |
container_volume |
1428 |
container_start_page |
115 |
op_container_end_page |
125 |
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1790603910443958272 |