Description of Holtermanniella takashimae sp. nov., Holtermanniella gen. nov. and proposal of the order Holtermanniales to accommodate Tremellomycetous yeasts of the Holtermannia clade.

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Wuczkowski M, Passoth V, Andersson AC, Olstorpe M, Laitila A, Theelen B, van Broock M, Prillinger H, Sterflinger K, Schnürer J, Boekhout T, Libkind D., TURCHETTI, BENEDETTA, BUZZINI, Pietro
• Other Authors: Wuczkowski, M, Passoth, V, Turchetti, Benedetta, Andersson, Ac, Olstorpe, M, Laitila, A, Theelen, B, van Broock, M, Buzzini, Pietro, Prillinger, H, Sterflinger, K, Schnürer, J, Boekhout, T, Libkind, D.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2011
• Subjects: yeast taxonomy; FATTY-ACID-COMPOSITION; BASIDIOMYCETOUS YEASTS; DIFFERENT TEMPERATURES; SEQUENCE-ANALYSIS; DIVERSITY; SYSTEMATICS; ANTARCTICA; TAXONOMY; WATER; D1/D2; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11391/903299; https://doi.org/10.1099/ijs.0.019737-0

The novel genus Holtermanniella is proposed here to accommodate four Cryptococcus species closely related to Holtermannia corniformis that are included in the Holtermannia clade (Basidiomycota, Agaricomycotina). Thus, four novel combinations are proposed: Holtermanniella nyarrowii comb. nov., Holtermanniella festucosa comb. nov., Holtermanniella mycelialis comb. nov. and Holtermanniella wattica comb. nov. In addition, a novel anamorphic yeast species was studied with 15 isolates obtained from different habitats around the world. Analysis of the sequences of the D1/D2 region of their large subunit rDNA showed that the novel species is placed phylogenetically within the Holtermannia clade of the Tremellomycetes (Agaricomycotina, Basidiomycota). PCR fingerprinting and sequencing of ITS1-5.8S-ITS2 showed genetic intraspecific variability among the strains: three groups were formed, which did not correlate with geographical origin or substrate. This novel species, designated the type species of Holtermanniella gen. nov., is described as Holtermanniella takashimae sp. nov.; the type strain is CBS 11174(T) (=HB 982(T) =DBVPG 8012(T)). The order Holtermanniales ord. nov. is proposed here to include Holtermannia (the type genus) and Holtermanniella.