Biocatalysed synthesis of sn-1,3-diacylglycerol oil from extra virgin olive oil

• Published in: Enzyme and Microbial Technology
• Main Authors: BLASI, FRANCESCA, COSSIGNANI, Lina, SIMONETTI, Maria Stella, DAMIANI, Pietro
• Other Authors: Blasi, Francesca, Cossignani, Lina, Simonetti, Maria Stella, Damiani, Pietro
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2007
• Subjects: sn-1; 3-Diacylglycerol; Extra virgin olive oil; Novozym 435; Lipozyme IM; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11391/158176; https://doi.org/10.1016/j.enzmictec.2007.06.005

Enzymatic synthesis of sn-1,3-diacylglycerols (sn-1,3-DAG) in two steps without isolation of the intermediates was investigated. Firstly ethanolysis of extra virgin olive oil (EVO) using immobilized non-regiospecific lipase from Candida antarctica (Novozym 435) was carried out to obtain glycerol (Gly) and fatty acid ethyl esters (FAEE). In the second step the ethanolysis products have been re-esterificated testing different sn-1,3-regiospecific lipases, both immobilized and non-immobilized, in different reaction media, that is in the presence of solvents or in a solvent-free system, for different times, at different temperatures (12, 25 and 40 °C). The lipase from Rhizomucor miehei (Lipozyme IM) has been the most effective among the sn-1,3-specific lipases screened.