Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin
International audience Pacific oysters (Crassostrea gigas) may bio-accumulate high levels of paralytic shellfish toxins (PST) during harmful algal blooms of the genus Alexandrium. These blooms regularly occur in coastal waters, affecting oyster health and marketability. The aim of our study was to a...
Published in: | Marine Drugs |
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Main Authors: | , , , , , |
Other Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2021
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Online Access: | https://hal.science/hal-03277599 https://hal.science/hal-03277599/document https://hal.science/hal-03277599/file/marinedrugs-19-00380-v2.pdf https://doi.org/10.3390/md19070380 |
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Archives ouvertes de Paris-Saclay |
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English |
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[SDV]Life Sciences [q-bio] [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [SDV.TOX]Life Sciences [q-bio]/Toxicology |
spellingShingle |
[SDV]Life Sciences [q-bio] [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [SDV.TOX]Life Sciences [q-bio]/Toxicology Boullot, Floriane Fabioux, Caroline Hégaret, Hélène Boudry, Pierre Soudant, Philippe Benoit, Evelyne Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin |
topic_facet |
[SDV]Life Sciences [q-bio] [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [SDV.TOX]Life Sciences [q-bio]/Toxicology |
description |
International audience Pacific oysters (Crassostrea gigas) may bio-accumulate high levels of paralytic shellfish toxins (PST) during harmful algal blooms of the genus Alexandrium. These blooms regularly occur in coastal waters, affecting oyster health and marketability. The aim of our study was to analyse the PST-sensitivity of nerves of Pacific oysters in relation with toxin bio-accumulation. The results show that C. gigas nerves have micromolar range of saxitoxin (STX) sensitivity, thus providing intermediate STX sensitivity compared to other bivalve species. However, theses nerves were much less sensitive to tetrodotoxin. The STX-sensitivity of compound nerve action potential (CNAP) recorded from oysters experimentally fed with Alexandrium minutum (toxic-alga-exposed oysters), or Tisochrysis lutea, a non-toxic microalga (control oysters), revealed that oysters could be separated into STX-resistant and STX-sensitive categories, regardless of the diet. Moreover, the percentage of toxin-sensitive nerves was lower, and the STX concentration necessary to inhibit 50% of CNAP higher, in recently toxic-alga-exposed oysters than in control bivalves. However, no obvious correlation was observed between nerve sensitivity to STX and the STX content in oyster digestive glands. None of the nerves isolated from wild and farmed oysters was detected to be sensitive to tetrodotoxin. In conclusion, this study highlights the good potential of cerebrovisceral nerves of Pacific oysters for electrophysiological and pharmacological studies. In addition, this study shows, for the first time, that C. gigas nerves have micromolar range of STX sensitivity. The STX sensitivity decreases, at least temporary, upon recent oyster exposure to dinoflagellates producing PST under natural, but not experimental environment. |
author2 |
Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS) Médicaments et Technologies pour la Santé (MTS) Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Servce d'ingénierie moléculaire des protéines (SIMOPRO) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) This research was funded by the French National Research Agency (ANR) 13-CESA-0019-ACCUTOX , the French Research Network PHYCOTOX, the French National Center for Scientific Research (CNRS), and the French Alternative Energies and Atomic Energy Commission (CEA). F.B. was supported by a pre-doctoral fellowship from the Région Bretagne and the Université de Bretagne Occidentale (UBO). ANR-13-CESA-0019,ACCUTOX,De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire pour l'homme dans son contexte sociétal(2013) |
format |
Article in Journal/Newspaper |
author |
Boullot, Floriane Fabioux, Caroline Hégaret, Hélène Boudry, Pierre Soudant, Philippe Benoit, Evelyne |
author_facet |
Boullot, Floriane Fabioux, Caroline Hégaret, Hélène Boudry, Pierre Soudant, Philippe Benoit, Evelyne |
author_sort |
Boullot, Floriane |
title |
Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin |
title_short |
Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin |
title_full |
Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin |
title_fullStr |
Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin |
title_full_unstemmed |
Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin |
title_sort |
electrophysiological evaluation of pacific oyster (crassostrea gigas) sensitivity to saxitoxin and tetrodotoxin |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.science/hal-03277599 https://hal.science/hal-03277599/document https://hal.science/hal-03277599/file/marinedrugs-19-00380-v2.pdf https://doi.org/10.3390/md19070380 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1660-3397 Marine drugs https://hal.science/hal-03277599 Marine drugs, 2021, 19-E380 (7), pp.1-17. ⟨10.3390/md19070380⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/md19070380 hal-03277599 https://hal.science/hal-03277599 https://hal.science/hal-03277599/document https://hal.science/hal-03277599/file/marinedrugs-19-00380-v2.pdf doi:10.3390/md19070380 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/md19070380 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
7 |
container_start_page |
380 |
_version_ |
1810440692925726720 |
spelling |
ftuniparissaclay:oai:HAL:hal-03277599v1 2024-09-15T18:03:11+00:00 Electrophysiological Evaluation of Pacific Oyster (Crassostrea gigas) Sensitivity to Saxitoxin and Tetrodotoxin Boullot, Floriane Fabioux, Caroline Hégaret, Hélène Boudry, Pierre Soudant, Philippe Benoit, Evelyne Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS) Médicaments et Technologies pour la Santé (MTS) Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Servce d'ingénierie moléculaire des protéines (SIMOPRO) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) This research was funded by the French National Research Agency (ANR) 13-CESA-0019-ACCUTOX , the French Research Network PHYCOTOX, the French National Center for Scientific Research (CNRS), and the French Alternative Energies and Atomic Energy Commission (CEA). F.B. was supported by a pre-doctoral fellowship from the Région Bretagne and the Université de Bretagne Occidentale (UBO). ANR-13-CESA-0019,ACCUTOX,De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire pour l'homme dans son contexte sociétal(2013) 2021-07 https://hal.science/hal-03277599 https://hal.science/hal-03277599/document https://hal.science/hal-03277599/file/marinedrugs-19-00380-v2.pdf https://doi.org/10.3390/md19070380 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/md19070380 hal-03277599 https://hal.science/hal-03277599 https://hal.science/hal-03277599/document https://hal.science/hal-03277599/file/marinedrugs-19-00380-v2.pdf doi:10.3390/md19070380 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1660-3397 Marine drugs https://hal.science/hal-03277599 Marine drugs, 2021, 19-E380 (7), pp.1-17. ⟨10.3390/md19070380⟩ [SDV]Life Sciences [q-bio] [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] [SDV.TOX]Life Sciences [q-bio]/Toxicology info:eu-repo/semantics/article Journal articles 2021 ftuniparissaclay https://doi.org/10.3390/md19070380 2024-08-22T23:46:22Z International audience Pacific oysters (Crassostrea gigas) may bio-accumulate high levels of paralytic shellfish toxins (PST) during harmful algal blooms of the genus Alexandrium. These blooms regularly occur in coastal waters, affecting oyster health and marketability. The aim of our study was to analyse the PST-sensitivity of nerves of Pacific oysters in relation with toxin bio-accumulation. The results show that C. gigas nerves have micromolar range of saxitoxin (STX) sensitivity, thus providing intermediate STX sensitivity compared to other bivalve species. However, theses nerves were much less sensitive to tetrodotoxin. The STX-sensitivity of compound nerve action potential (CNAP) recorded from oysters experimentally fed with Alexandrium minutum (toxic-alga-exposed oysters), or Tisochrysis lutea, a non-toxic microalga (control oysters), revealed that oysters could be separated into STX-resistant and STX-sensitive categories, regardless of the diet. Moreover, the percentage of toxin-sensitive nerves was lower, and the STX concentration necessary to inhibit 50% of CNAP higher, in recently toxic-alga-exposed oysters than in control bivalves. However, no obvious correlation was observed between nerve sensitivity to STX and the STX content in oyster digestive glands. None of the nerves isolated from wild and farmed oysters was detected to be sensitive to tetrodotoxin. In conclusion, this study highlights the good potential of cerebrovisceral nerves of Pacific oysters for electrophysiological and pharmacological studies. In addition, this study shows, for the first time, that C. gigas nerves have micromolar range of STX sensitivity. The STX sensitivity decreases, at least temporary, upon recent oyster exposure to dinoflagellates producing PST under natural, but not experimental environment. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archives ouvertes de Paris-Saclay Marine Drugs 19 7 380 |