Fine mapping of a salmonid E2 alphavirus neutralizing epitope
International audience In this study, we aimed to characterize the epitope recognized by the neutralizing 17H23 mAb directed against the E2 glycoprotein of most of salmonid alphavirus (SAV) subtypes and widely used in several laboratories to routinely diagnose SAV. We hypothesized that the 17H23 epi...
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ftuniparissaclay:oai:HAL:hal-02639636v1 2024-06-23T07:51:24+00:00 Fine mapping of a salmonid E2 alphavirus neutralizing epitope Mérour, Emilie Lamoureux, Annie Biacchesi, Stephane Bremont, Michel Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) Institut National de la Recherche Agronomique (INRA) Université Paris Saclay (COmUE) National Research Agency ANR 11 BSV302302 2016 https://hal.inrae.fr/hal-02639636 https://doi.org/10.1099/jgv.0.000411 en eng HAL CCSD Microbiology Society info:eu-repo/semantics/altIdentifier/doi/10.1099/jgv.0.000411 info:eu-repo/semantics/altIdentifier/pmid/26801972 hal-02639636 https://hal.inrae.fr/hal-02639636 doi:10.1099/jgv.0.000411 PRODINRA: 354856 PUBMED: 26801972 WOS: 000374108900009 ISSN: 0022-1317 EISSN: 1465-2099 Journal of General Virology https://hal.inrae.fr/hal-02639636 Journal of General Virology, 2016, 97, pp.893-900. ⟨10.1099/jgv.0.000411⟩ sleeping-disease-virus atlantic salmon transmission pancreas disease rainbow-trout antibodies vertical glycoprotein infections [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2016 ftuniparissaclay https://doi.org/10.1099/jgv.0.000411 2024-06-06T23:44:08Z International audience In this study, we aimed to characterize the epitope recognized by the neutralizing 17H23 mAb directed against the E2 glycoprotein of most of salmonid alphavirus (SAV) subtypes and widely used in several laboratories to routinely diagnose SAV. We hypothesized that the 17H23 epitope was located in the major domain B, previously identified in the E2 of mammalian alphaviruses as the domain recognized by most of the E2 neutralizing mAbs. Indeed, the SAV E2 domain B counterpart is contained in the protein domain previously characterized as being recognized by mAb 17H23. Thus, to precisely characterize the 17H23 epitope, we developed an alanine scanning mutagenesis approach coupled with the generation of the respective recombinant SAV (rSAV) by using the available infectious cDNA. Ten mutant rSAVs termed A-J from E2 aa 223-236 were produced and characterized in vitro using indirect immunofluorescence assays on virus-infected cells with mAbs 17H23, 51B8 (another non-neutralizing anti-E2 mAb) and 19F3 directed against the non-structural protein nsp1. Two of the mutant rSAVs (G and H) escaped neutralization by mAb 17H23. In addition, we showed that when juvenile trout were infected by bath immersion with the rSAV mutants, some of them were either totally (D, E and G) or partially (H) attenuated. Together, the data from the in vitro and in vivo experiments indicated that the putative 17H23 amino acid sequence epitope comprised the short amino acid sequence (FTSDS231)-F-227. Article in Journal/Newspaper Atlantic salmon Archives ouvertes de Paris-Saclay Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Journal of General Virology 97 4 893 900 |
institution |
Open Polar |
collection |
Archives ouvertes de Paris-Saclay |
op_collection_id |
ftuniparissaclay |
language |
English |
topic |
sleeping-disease-virus atlantic salmon transmission pancreas disease rainbow-trout antibodies vertical glycoprotein infections [SDV]Life Sciences [q-bio] |
spellingShingle |
sleeping-disease-virus atlantic salmon transmission pancreas disease rainbow-trout antibodies vertical glycoprotein infections [SDV]Life Sciences [q-bio] Mérour, Emilie Lamoureux, Annie Biacchesi, Stephane Bremont, Michel Fine mapping of a salmonid E2 alphavirus neutralizing epitope |
topic_facet |
sleeping-disease-virus atlantic salmon transmission pancreas disease rainbow-trout antibodies vertical glycoprotein infections [SDV]Life Sciences [q-bio] |
description |
International audience In this study, we aimed to characterize the epitope recognized by the neutralizing 17H23 mAb directed against the E2 glycoprotein of most of salmonid alphavirus (SAV) subtypes and widely used in several laboratories to routinely diagnose SAV. We hypothesized that the 17H23 epitope was located in the major domain B, previously identified in the E2 of mammalian alphaviruses as the domain recognized by most of the E2 neutralizing mAbs. Indeed, the SAV E2 domain B counterpart is contained in the protein domain previously characterized as being recognized by mAb 17H23. Thus, to precisely characterize the 17H23 epitope, we developed an alanine scanning mutagenesis approach coupled with the generation of the respective recombinant SAV (rSAV) by using the available infectious cDNA. Ten mutant rSAVs termed A-J from E2 aa 223-236 were produced and characterized in vitro using indirect immunofluorescence assays on virus-infected cells with mAbs 17H23, 51B8 (another non-neutralizing anti-E2 mAb) and 19F3 directed against the non-structural protein nsp1. Two of the mutant rSAVs (G and H) escaped neutralization by mAb 17H23. In addition, we showed that when juvenile trout were infected by bath immersion with the rSAV mutants, some of them were either totally (D, E and G) or partially (H) attenuated. Together, the data from the in vitro and in vivo experiments indicated that the putative 17H23 amino acid sequence epitope comprised the short amino acid sequence (FTSDS231)-F-227. |
author2 |
Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) Institut National de la Recherche Agronomique (INRA) Université Paris Saclay (COmUE) National Research Agency ANR 11 BSV302302 |
format |
Article in Journal/Newspaper |
author |
Mérour, Emilie Lamoureux, Annie Biacchesi, Stephane Bremont, Michel |
author_facet |
Mérour, Emilie Lamoureux, Annie Biacchesi, Stephane Bremont, Michel |
author_sort |
Mérour, Emilie |
title |
Fine mapping of a salmonid E2 alphavirus neutralizing epitope |
title_short |
Fine mapping of a salmonid E2 alphavirus neutralizing epitope |
title_full |
Fine mapping of a salmonid E2 alphavirus neutralizing epitope |
title_fullStr |
Fine mapping of a salmonid E2 alphavirus neutralizing epitope |
title_full_unstemmed |
Fine mapping of a salmonid E2 alphavirus neutralizing epitope |
title_sort |
fine mapping of a salmonid e2 alphavirus neutralizing epitope |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.inrae.fr/hal-02639636 https://doi.org/10.1099/jgv.0.000411 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
ISSN: 0022-1317 EISSN: 1465-2099 Journal of General Virology https://hal.inrae.fr/hal-02639636 Journal of General Virology, 2016, 97, pp.893-900. ⟨10.1099/jgv.0.000411⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1099/jgv.0.000411 info:eu-repo/semantics/altIdentifier/pmid/26801972 hal-02639636 https://hal.inrae.fr/hal-02639636 doi:10.1099/jgv.0.000411 PRODINRA: 354856 PUBMED: 26801972 WOS: 000374108900009 |
op_doi |
https://doi.org/10.1099/jgv.0.000411 |
container_title |
Journal of General Virology |
container_volume |
97 |
container_issue |
4 |
container_start_page |
893 |
op_container_end_page |
900 |
_version_ |
1802642501217026048 |