Different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( Scophthalmus maximus L.) head-kidney leucocytes
International audience In aquaculture, several criteria should be considered to select an appropriate probiotic, including the aquatic origin and safety of the strain and its ability to modulate the host immune response. The properties and effects of probiotics are strain-specific and some factors s...
Published in: | Fish & Shellfish Immunology |
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ftuniparissaclay:oai:HAL:hal-02636011v1 2024-05-12T08:10:41+00:00 Different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( Scophthalmus maximus L.) head-kidney leucocytes Munoz-Atienza, Estefania Araujo, Carlos Lluch, Nuria Hernandez, Pablo E. Herranz, Carmen Cintas, Luis M. Magadan, Susana Universidad Complutense de Madrid = Complutense University of Madrid Madrid (UCM) Universidade de Trás-os-Montes e Alto Douro Centro Oceanográfico de Vigo Instituto Espagňol de Oceanografia (IEO) Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC)-Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC) Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) Institut National de la Recherche Agronomique (INRA) Ministerio de Economia y Competitividad (MINECO) AGL2012-34829 TEBAMOL from Centro Oceanografico de Vigo, Instituto Espanol de Oceanografia (IEO); Direccion General de Universidades e Investigacion S-2009/AGR-1489, P2013/ABI-2747 Consejeria de Educacion; Comunidad de Madrid, Spain; Universidad Complutense de Madrid (Madrid, Spain); Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) SFRH/BD/62416/2009 European Social Fund (ESF) SFRH/BD/62416/2009 General Direction of Research, Development and Innovation of Xunta de Galicia (Spain) 2015 https://hal.inrae.fr/hal-02636011 https://doi.org/10.1016/j.fsi.2015.02.021 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2015.02.021 info:eu-repo/semantics/altIdentifier/pmid/25707601 hal-02636011 https://hal.inrae.fr/hal-02636011 doi:10.1016/j.fsi.2015.02.021 PRODINRA: 329275 PUBMED: 25707601 WOS: 000353743900025 ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.inrae.fr/hal-02636011 Fish and Shellfish Immunology, 2015, 44 (1), pp.214-223. ⟨10.1016/j.fsi.2015.02.021⟩ Fish probiotics Lactic acid bacteria In vitro immunomodulation Turbot head-kidney leucocytes Apoptosis [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2015 ftuniparissaclay https://doi.org/10.1016/j.fsi.2015.02.021 2024-04-15T17:43:16Z International audience In aquaculture, several criteria should be considered to select an appropriate probiotic, including the aquatic origin and safety of the strain and its ability to modulate the host immune response. The properties and effects of probiotics are strain-specific and some factors such as viability, dose and duration of diet supplementation may regulate their immunomodulatory activities. In this study, we assessed the in vitro effect of eight heat-inactivated and viable lactic acid bacteria (LAB) of aquatic origin belonging to the genera Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Weissella on the viability and innate immune response of turbot (Scophthalmus maximus L.) leucocytes. Head-kidney leucocytes were incubated with viable and heat-inactivated Lat different concentrations. After incubation, the viability of leucocytes was evaluated using colorimetric assays (MIT and LDH) and flow cytometry (annexin V/propidium iodide). Heat-inactivated Lshowed no cytotoxic effect while viable Lexerted variable influence on apoptosis of turbot phagocytes and lymphocytes. Leucocyte respiratory burst activity and phagocytosis were also differentially activated, as viable Lstimulated leucocytes more efficiently than the heat-inactivated LAB. Our results suggest diverse strain-specific mechanisms of interaction between the evaluated Land turbot leucocytes. Furthermore, our work sets up in vitro systems to evaluate the effect of Las potential probiotics, which will be useful to develop efficient screening. Article in Journal/Newspaper Scophthalmus maximus Turbot Archives ouvertes de Paris-Saclay Fish & Shellfish Immunology 44 1 214 223 |
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collection |
Archives ouvertes de Paris-Saclay |
op_collection_id |
ftuniparissaclay |
language |
English |
topic |
Fish probiotics Lactic acid bacteria In vitro immunomodulation Turbot head-kidney leucocytes Apoptosis [SDV]Life Sciences [q-bio] |
spellingShingle |
Fish probiotics Lactic acid bacteria In vitro immunomodulation Turbot head-kidney leucocytes Apoptosis [SDV]Life Sciences [q-bio] Munoz-Atienza, Estefania Araujo, Carlos Lluch, Nuria Hernandez, Pablo E. Herranz, Carmen Cintas, Luis M. Magadan, Susana Different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( Scophthalmus maximus L.) head-kidney leucocytes |
topic_facet |
Fish probiotics Lactic acid bacteria In vitro immunomodulation Turbot head-kidney leucocytes Apoptosis [SDV]Life Sciences [q-bio] |
description |
International audience In aquaculture, several criteria should be considered to select an appropriate probiotic, including the aquatic origin and safety of the strain and its ability to modulate the host immune response. The properties and effects of probiotics are strain-specific and some factors such as viability, dose and duration of diet supplementation may regulate their immunomodulatory activities. In this study, we assessed the in vitro effect of eight heat-inactivated and viable lactic acid bacteria (LAB) of aquatic origin belonging to the genera Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Weissella on the viability and innate immune response of turbot (Scophthalmus maximus L.) leucocytes. Head-kidney leucocytes were incubated with viable and heat-inactivated Lat different concentrations. After incubation, the viability of leucocytes was evaluated using colorimetric assays (MIT and LDH) and flow cytometry (annexin V/propidium iodide). Heat-inactivated Lshowed no cytotoxic effect while viable Lexerted variable influence on apoptosis of turbot phagocytes and lymphocytes. Leucocyte respiratory burst activity and phagocytosis were also differentially activated, as viable Lstimulated leucocytes more efficiently than the heat-inactivated LAB. Our results suggest diverse strain-specific mechanisms of interaction between the evaluated Land turbot leucocytes. Furthermore, our work sets up in vitro systems to evaluate the effect of Las potential probiotics, which will be useful to develop efficient screening. |
author2 |
Universidad Complutense de Madrid = Complutense University of Madrid Madrid (UCM) Universidade de Trás-os-Montes e Alto Douro Centro Oceanográfico de Vigo Instituto Espagňol de Oceanografia (IEO) Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC)-Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC) Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) Institut National de la Recherche Agronomique (INRA) Ministerio de Economia y Competitividad (MINECO) AGL2012-34829 TEBAMOL from Centro Oceanografico de Vigo, Instituto Espanol de Oceanografia (IEO); Direccion General de Universidades e Investigacion S-2009/AGR-1489, P2013/ABI-2747 Consejeria de Educacion; Comunidad de Madrid, Spain; Universidad Complutense de Madrid (Madrid, Spain); Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) SFRH/BD/62416/2009 European Social Fund (ESF) SFRH/BD/62416/2009 General Direction of Research, Development and Innovation of Xunta de Galicia (Spain) |
format |
Article in Journal/Newspaper |
author |
Munoz-Atienza, Estefania Araujo, Carlos Lluch, Nuria Hernandez, Pablo E. Herranz, Carmen Cintas, Luis M. Magadan, Susana |
author_facet |
Munoz-Atienza, Estefania Araujo, Carlos Lluch, Nuria Hernandez, Pablo E. Herranz, Carmen Cintas, Luis M. Magadan, Susana |
author_sort |
Munoz-Atienza, Estefania |
title |
Different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( Scophthalmus maximus L.) head-kidney leucocytes |
title_short |
Different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( Scophthalmus maximus L.) head-kidney leucocytes |
title_full |
Different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( Scophthalmus maximus L.) head-kidney leucocytes |
title_fullStr |
Different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( Scophthalmus maximus L.) head-kidney leucocytes |
title_full_unstemmed |
Different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( Scophthalmus maximus L.) head-kidney leucocytes |
title_sort |
different impact of heat-inactivated and viable lactic acid bacteria of aquatic origin on turbot ( scophthalmus maximus l.) head-kidney leucocytes |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.inrae.fr/hal-02636011 https://doi.org/10.1016/j.fsi.2015.02.021 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology https://hal.inrae.fr/hal-02636011 Fish and Shellfish Immunology, 2015, 44 (1), pp.214-223. ⟨10.1016/j.fsi.2015.02.021⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2015.02.021 info:eu-repo/semantics/altIdentifier/pmid/25707601 hal-02636011 https://hal.inrae.fr/hal-02636011 doi:10.1016/j.fsi.2015.02.021 PRODINRA: 329275 PUBMED: 25707601 WOS: 000353743900025 |
op_doi |
https://doi.org/10.1016/j.fsi.2015.02.021 |
container_title |
Fish & Shellfish Immunology |
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44 |
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214 |
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223 |
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