Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene
International audience BACKGROUNDIn spite of intensive use of bromadiolone, rodent control was inefficient on a farm infested by rats in Zaragoza, Spain. While metabolic resistance was previously described in this rodent species, the observation of a target resistance to antivitamin K rodenticides h...
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Online Access: | https://hal.inrae.fr/hal-02630922 https://doi.org/10.1002/ps.4020 |
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ftuniparissaclay:oai:HAL:hal-02630922v1 2024-01-14T10:10:09+01:00 Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene Goulois, Joffrey Chapuzet, Audrey Lambert, Véronique Chatron, Nolan Tchertanov, Luba Legros, Lionel Benoit, Etienne Lattard, Virginie Mycotoxines et Toxicologie Comparée des Xénobiotiques (MET) Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Lyon (ENVL) Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP) Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) École normale supérieure - Cachan (ENS Cachan) Liphatech, Bonnel, France Partenaires INRAE Liphatech ISI grant from Bpi France I1301001W 2016 https://hal.inrae.fr/hal-02630922 https://doi.org/10.1002/ps.4020 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1002/ps.4020 info:eu-repo/semantics/altIdentifier/pmid/25847836 hal-02630922 https://hal.inrae.fr/hal-02630922 doi:10.1002/ps.4020 PRODINRA: 356087 PUBMED: 25847836 WOS: 000369724500013 ISSN: 1526-498X EISSN: 1526-4998 Pest Management Science https://hal.inrae.fr/hal-02630922 Pest Management Science, 2016, 72 (3), pp.544 - 550. ⟨10.1002/ps.4020⟩ antivitamin K VKORC1 resistance rattus rattus mutation [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2016 ftuniparissaclay https://doi.org/10.1002/ps.4020 2023-12-16T22:38:30Z International audience BACKGROUNDIn spite of intensive use of bromadiolone, rodent control was inefficient on a farm infested by rats in Zaragoza, Spain. While metabolic resistance was previously described in this rodent species, the observation of a target resistance to antivitamin K rodenticides had been poorly documented in Rattus rattus. RESULTSFrom rats trapped on the farm, cytochrome b and Vkorc1 genes were amplified by PCR and sequenced in order to identify species and detect potential Vkorc1 mutations. VKORC1-deduced amino acid sequences were thus expressed in Pichia pastoris, and inhibition constants towards various rodenticides were determined. The ten rats trapped on the farm were all identified as R. rattus. They were found to be homozygous for the g.74A>T nucleotide replacement in exon 1 of the Vkorc1 gene, leading to p.Y25F mutation. This mutation led to increased apparent inhibition constants towards various rodenticides, probably caused by a partial loss of helical structure of TM4. CONCLUSIONThe p.Y25F mutation detected in the Vkorc1 gene in R. rattus trapped on the Spanish farm is associated with the resistance phenotype to bromadiolone that has been observed. It is the first evidence of target resistance to antivitamin K anticoagulants in R. rattus. Article in Journal/Newspaper Rattus rattus Archives ouvertes de Paris-Saclay Pest Management Science 72 3 544 550 |
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Open Polar |
collection |
Archives ouvertes de Paris-Saclay |
op_collection_id |
ftuniparissaclay |
language |
English |
topic |
antivitamin K VKORC1 resistance rattus rattus mutation [SDV]Life Sciences [q-bio] |
spellingShingle |
antivitamin K VKORC1 resistance rattus rattus mutation [SDV]Life Sciences [q-bio] Goulois, Joffrey Chapuzet, Audrey Lambert, Véronique Chatron, Nolan Tchertanov, Luba Legros, Lionel Benoit, Etienne Lattard, Virginie Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene |
topic_facet |
antivitamin K VKORC1 resistance rattus rattus mutation [SDV]Life Sciences [q-bio] |
description |
International audience BACKGROUNDIn spite of intensive use of bromadiolone, rodent control was inefficient on a farm infested by rats in Zaragoza, Spain. While metabolic resistance was previously described in this rodent species, the observation of a target resistance to antivitamin K rodenticides had been poorly documented in Rattus rattus. RESULTSFrom rats trapped on the farm, cytochrome b and Vkorc1 genes were amplified by PCR and sequenced in order to identify species and detect potential Vkorc1 mutations. VKORC1-deduced amino acid sequences were thus expressed in Pichia pastoris, and inhibition constants towards various rodenticides were determined. The ten rats trapped on the farm were all identified as R. rattus. They were found to be homozygous for the g.74A>T nucleotide replacement in exon 1 of the Vkorc1 gene, leading to p.Y25F mutation. This mutation led to increased apparent inhibition constants towards various rodenticides, probably caused by a partial loss of helical structure of TM4. CONCLUSIONThe p.Y25F mutation detected in the Vkorc1 gene in R. rattus trapped on the Spanish farm is associated with the resistance phenotype to bromadiolone that has been observed. It is the first evidence of target resistance to antivitamin K anticoagulants in R. rattus. |
author2 |
Mycotoxines et Toxicologie Comparée des Xénobiotiques (MET) Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Lyon (ENVL) Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP) Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) École normale supérieure - Cachan (ENS Cachan) Liphatech, Bonnel, France Partenaires INRAE Liphatech ISI grant from Bpi France I1301001W |
format |
Article in Journal/Newspaper |
author |
Goulois, Joffrey Chapuzet, Audrey Lambert, Véronique Chatron, Nolan Tchertanov, Luba Legros, Lionel Benoit, Etienne Lattard, Virginie |
author_facet |
Goulois, Joffrey Chapuzet, Audrey Lambert, Véronique Chatron, Nolan Tchertanov, Luba Legros, Lionel Benoit, Etienne Lattard, Virginie |
author_sort |
Goulois, Joffrey |
title |
Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene |
title_short |
Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene |
title_full |
Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene |
title_fullStr |
Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene |
title_full_unstemmed |
Evidence of a target resistance to antivitamin K rodenticides in the roof rat Rattus rattus: identification and characterisation of a novel Y25F mutation in the Vkorc1 gene |
title_sort |
evidence of a target resistance to antivitamin k rodenticides in the roof rat rattus rattus: identification and characterisation of a novel y25f mutation in the vkorc1 gene |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.inrae.fr/hal-02630922 https://doi.org/10.1002/ps.4020 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
ISSN: 1526-498X EISSN: 1526-4998 Pest Management Science https://hal.inrae.fr/hal-02630922 Pest Management Science, 2016, 72 (3), pp.544 - 550. ⟨10.1002/ps.4020⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/ps.4020 info:eu-repo/semantics/altIdentifier/pmid/25847836 hal-02630922 https://hal.inrae.fr/hal-02630922 doi:10.1002/ps.4020 PRODINRA: 356087 PUBMED: 25847836 WOS: 000369724500013 |
op_doi |
https://doi.org/10.1002/ps.4020 |
container_title |
Pest Management Science |
container_volume |
72 |
container_issue |
3 |
container_start_page |
544 |
op_container_end_page |
550 |
_version_ |
1788064777333899264 |