Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers
The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the Un...
Published in: | Biological Invasions |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2015
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Subjects: | |
Online Access: | https://hal.science/hal-01535289 https://doi.org/10.1007/s10530-015-0896-1 |
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ftuniparissaclay:oai:HAL:hal-01535289v1 |
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openpolar |
institution |
Open Polar |
collection |
Archives ouvertes de Paris-Saclay |
op_collection_id |
ftuniparissaclay |
language |
English |
topic |
mtDNA Pacific oyster Crassostrea gigas Invasion genetics Marine mollusc Microsatellites ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
mtDNA Pacific oyster Crassostrea gigas Invasion genetics Marine mollusc Microsatellites ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Lallias, Delphine Boudry, Pierre Batista, Frederico M. Beaumont, Andy King, Jonathan W. Turner, John R. Lapègue, Sylvie Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers |
topic_facet |
mtDNA Pacific oyster Crassostrea gigas Invasion genetics Marine mollusc Microsatellites ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and Denmark as well as UK hatchery stocks) and (2) a Southwest cluster (including samples from South Wales, South West England, France and Spain). The Southwest cluster had significantly higher allelic richness (A) and expected heterozygosity (H-e ) (A: 45.68, H-e : 0.928) than in the Northeast (A: 26.58, H-e : 0.883); the two diverging by a small but significant F-ST value (F-ST = 0.017, 95 % CI 0.014-0.021). A 739-bp fragment of the major noncoding region of the mitochondrial genome was sequenced in 248 oysters from 12 of the studied samples in Europe and in 25 oysters from Miyagi prefecture (Japan). A total of 81 haplotypes were found. Haplotype frequency analyses identified the same two clusters observed using microsatellites. This study highlights how the number and size of introduction events, aquaculture practices, genetic bottlenecks followed by genetic drift and natural dispersal can act concurrently to shape the genetic diversity and structure of introduced populations. |
author2 |
School of Ocean Sciences Menai Bridge Bangor University Génétique Animale et Biologie Intégrative (GABI) Institut National de la Recherche Agronomique (INRA)-AgroParisTech Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Estação Experimental de Moluscicultura de Tavira, Divisão de Aquicultura e Valorização Instituto Português de Investigação do Mar e da Atmosfera (IPMA) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) European Union 2009-1/123 |
format |
Article in Journal/Newspaper |
author |
Lallias, Delphine Boudry, Pierre Batista, Frederico M. Beaumont, Andy King, Jonathan W. Turner, John R. Lapègue, Sylvie |
author_facet |
Lallias, Delphine Boudry, Pierre Batista, Frederico M. Beaumont, Andy King, Jonathan W. Turner, John R. Lapègue, Sylvie |
author_sort |
Lallias, Delphine |
title |
Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers |
title_short |
Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers |
title_full |
Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers |
title_fullStr |
Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers |
title_full_unstemmed |
Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers |
title_sort |
invasion genetics of the pacific oyster crassostrea gigas in the british isles inferred from microsatellite and mitochondrial markers |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-01535289 https://doi.org/10.1007/s10530-015-0896-1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1387-3547 EISSN: 1573-1464 Biological Invasions https://hal.science/hal-01535289 Biological Invasions, 2015, 17 (9), pp.2581 - 2595. ⟨10.1007/s10530-015-0896-1⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10530-015-0896-1 hal-01535289 https://hal.science/hal-01535289 doi:10.1007/s10530-015-0896-1 PRODINRA: 336523 WOS: 000359427400007 |
op_doi |
https://doi.org/10.1007/s10530-015-0896-1 |
container_title |
Biological Invasions |
container_volume |
17 |
container_issue |
9 |
container_start_page |
2581 |
op_container_end_page |
2595 |
_version_ |
1809906496358580224 |
spelling |
ftuniparissaclay:oai:HAL:hal-01535289v1 2024-09-09T19:37:18+00:00 Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers Lallias, Delphine Boudry, Pierre Batista, Frederico M. Beaumont, Andy King, Jonathan W. Turner, John R. Lapègue, Sylvie School of Ocean Sciences Menai Bridge Bangor University Génétique Animale et Biologie Intégrative (GABI) Institut National de la Recherche Agronomique (INRA)-AgroParisTech Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Estação Experimental de Moluscicultura de Tavira, Divisão de Aquicultura e Valorização Instituto Português de Investigação do Mar e da Atmosfera (IPMA) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) European Union 2009-1/123 2015-09 https://hal.science/hal-01535289 https://doi.org/10.1007/s10530-015-0896-1 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10530-015-0896-1 hal-01535289 https://hal.science/hal-01535289 doi:10.1007/s10530-015-0896-1 PRODINRA: 336523 WOS: 000359427400007 ISSN: 1387-3547 EISSN: 1573-1464 Biological Invasions https://hal.science/hal-01535289 Biological Invasions, 2015, 17 (9), pp.2581 - 2595. ⟨10.1007/s10530-015-0896-1⟩ mtDNA Pacific oyster Crassostrea gigas Invasion genetics Marine mollusc Microsatellites ACL [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2015 ftuniparissaclay https://doi.org/10.1007/s10530-015-0896-1 2024-06-20T23:46:03Z The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and Denmark as well as UK hatchery stocks) and (2) a Southwest cluster (including samples from South Wales, South West England, France and Spain). The Southwest cluster had significantly higher allelic richness (A) and expected heterozygosity (H-e ) (A: 45.68, H-e : 0.928) than in the Northeast (A: 26.58, H-e : 0.883); the two diverging by a small but significant F-ST value (F-ST = 0.017, 95 % CI 0.014-0.021). A 739-bp fragment of the major noncoding region of the mitochondrial genome was sequenced in 248 oysters from 12 of the studied samples in Europe and in 25 oysters from Miyagi prefecture (Japan). A total of 81 haplotypes were found. Haplotype frequency analyses identified the same two clusters observed using microsatellites. This study highlights how the number and size of introduction events, aquaculture practices, genetic bottlenecks followed by genetic drift and natural dispersal can act concurrently to shape the genetic diversity and structure of introduced populations. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archives ouvertes de Paris-Saclay Pacific Biological Invasions 17 9 2581 2595 |