Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria.
International audience From the mandibular lymph nodes of wild red foxes (Vulpes vulpes) hunted in the region of Gmünd, Lower Austria, two gram-negative, oxidase- and urease-positive, coccoid rod-shaped bacteria (strains 257 and 284) were isolated. Cells were fast growing, nonmotile, and agglutinate...
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ftuniparissaclay:oai:HAL:hal-00532594v1 2024-09-15T18:18:46+00:00 Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. Scholz, Holger Christian Hofer, Erwin Vergnaud, Gilles Le Fleche, Philippe Whatmore, Adrian M Al Dahouk, Sascha Pfeffer, Martin Krüger, Monika Cloeckaert, Axel Tomaso, Herbert Institut de génétique et microbiologie Orsay (IGM) Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) 2009-04 https://hal.science/hal-00532594 https://doi.org/10.1089/vbz.2008.0036 en eng HAL CCSD Mary Ann Liebert info:eu-repo/semantics/altIdentifier/doi/10.1089/vbz.2008.0036 info:eu-repo/semantics/altIdentifier/pmid/18973444 hal-00532594 https://hal.science/hal-00532594 doi:10.1089/vbz.2008.0036 PRODINRA: 148823 PUBMED: 18973444 WOS: 000265416200004 ISSN: 1530-3667 Vector-Borne and Zoonotic Diseases https://hal.science/hal-00532594 Vector-Borne and Zoonotic Diseases, 2009, 9 (2), pp.153-6. ⟨10.1089/vbz.2008.0036⟩ MESH: Animals MESH: Austria MESH: Mandible MESH: Ochrobactrum anthropi MESH: RNA Ribosomal 16S MESH: Rec A Recombinases MESH: Sequence Analysis MESH: Bacterial Outer Membrane Proteins MESH: Brucella MESH: Brucellosis MESH: Czech Republic MESH: Foxes MESH: Genotype MESH: Geography MESH: Lymph Nodes [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2009 ftuniparissaclay https://doi.org/10.1089/vbz.2008.0036 2024-08-30T01:48:44Z International audience From the mandibular lymph nodes of wild red foxes (Vulpes vulpes) hunted in the region of Gmünd, Lower Austria, two gram-negative, oxidase- and urease-positive, coccoid rod-shaped bacteria (strains 257 and 284) were isolated. Cells were fast growing, nonmotile, and agglutinated with monospecific anti-Brucella (M) serum. Both strains were biochemically identified as Ochrobactrum anthropi by using the API 20NE test. However, sequencing of the 16S rRNA and recA genes clearly identified strains 257 and 284 as Brucella spp. Further molecular analysis by omp2a/b gene sequencing, multilocus sequence typing and multilocus variable number tandem repeats analysis revealed Brucella microti, a recently described Brucella species that has originally been isolated from diseased common voles (Microtus arvalis) in South Moravia, Czech Republic in 2000. Our findings demonstrate that B. microti is prevalent in a larger geographic area covering the region of South Moravia and parts of Lower Austria. Foxes could have become infected by ingestion of infected common voles. Article in Journal/Newspaper Microtus arvalis Archives ouvertes de Paris-Saclay Vector-Borne and Zoonotic Diseases 9 2 153 156 |
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Open Polar |
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Archives ouvertes de Paris-Saclay |
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ftuniparissaclay |
language |
English |
topic |
MESH: Animals MESH: Austria MESH: Mandible MESH: Ochrobactrum anthropi MESH: RNA Ribosomal 16S MESH: Rec A Recombinases MESH: Sequence Analysis MESH: Bacterial Outer Membrane Proteins MESH: Brucella MESH: Brucellosis MESH: Czech Republic MESH: Foxes MESH: Genotype MESH: Geography MESH: Lymph Nodes [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
MESH: Animals MESH: Austria MESH: Mandible MESH: Ochrobactrum anthropi MESH: RNA Ribosomal 16S MESH: Rec A Recombinases MESH: Sequence Analysis MESH: Bacterial Outer Membrane Proteins MESH: Brucella MESH: Brucellosis MESH: Czech Republic MESH: Foxes MESH: Genotype MESH: Geography MESH: Lymph Nodes [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Scholz, Holger Christian Hofer, Erwin Vergnaud, Gilles Le Fleche, Philippe Whatmore, Adrian M Al Dahouk, Sascha Pfeffer, Martin Krüger, Monika Cloeckaert, Axel Tomaso, Herbert Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. |
topic_facet |
MESH: Animals MESH: Austria MESH: Mandible MESH: Ochrobactrum anthropi MESH: RNA Ribosomal 16S MESH: Rec A Recombinases MESH: Sequence Analysis MESH: Bacterial Outer Membrane Proteins MESH: Brucella MESH: Brucellosis MESH: Czech Republic MESH: Foxes MESH: Genotype MESH: Geography MESH: Lymph Nodes [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
International audience From the mandibular lymph nodes of wild red foxes (Vulpes vulpes) hunted in the region of Gmünd, Lower Austria, two gram-negative, oxidase- and urease-positive, coccoid rod-shaped bacteria (strains 257 and 284) were isolated. Cells were fast growing, nonmotile, and agglutinated with monospecific anti-Brucella (M) serum. Both strains were biochemically identified as Ochrobactrum anthropi by using the API 20NE test. However, sequencing of the 16S rRNA and recA genes clearly identified strains 257 and 284 as Brucella spp. Further molecular analysis by omp2a/b gene sequencing, multilocus sequence typing and multilocus variable number tandem repeats analysis revealed Brucella microti, a recently described Brucella species that has originally been isolated from diseased common voles (Microtus arvalis) in South Moravia, Czech Republic in 2000. Our findings demonstrate that B. microti is prevalent in a larger geographic area covering the region of South Moravia and parts of Lower Austria. Foxes could have become infected by ingestion of infected common voles. |
author2 |
Institut de génétique et microbiologie Orsay (IGM) Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Scholz, Holger Christian Hofer, Erwin Vergnaud, Gilles Le Fleche, Philippe Whatmore, Adrian M Al Dahouk, Sascha Pfeffer, Martin Krüger, Monika Cloeckaert, Axel Tomaso, Herbert |
author_facet |
Scholz, Holger Christian Hofer, Erwin Vergnaud, Gilles Le Fleche, Philippe Whatmore, Adrian M Al Dahouk, Sascha Pfeffer, Martin Krüger, Monika Cloeckaert, Axel Tomaso, Herbert |
author_sort |
Scholz, Holger Christian |
title |
Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. |
title_short |
Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. |
title_full |
Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. |
title_fullStr |
Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. |
title_full_unstemmed |
Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. |
title_sort |
isolation of brucella microti from mandibular lymph nodes of red foxes, vulpes vulpes, in lower austria. |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.science/hal-00532594 https://doi.org/10.1089/vbz.2008.0036 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
ISSN: 1530-3667 Vector-Borne and Zoonotic Diseases https://hal.science/hal-00532594 Vector-Borne and Zoonotic Diseases, 2009, 9 (2), pp.153-6. ⟨10.1089/vbz.2008.0036⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1089/vbz.2008.0036 info:eu-repo/semantics/altIdentifier/pmid/18973444 hal-00532594 https://hal.science/hal-00532594 doi:10.1089/vbz.2008.0036 PRODINRA: 148823 PUBMED: 18973444 WOS: 000265416200004 |
op_doi |
https://doi.org/10.1089/vbz.2008.0036 |
container_title |
Vector-Borne and Zoonotic Diseases |
container_volume |
9 |
container_issue |
2 |
container_start_page |
153 |
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156 |
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1810456852217987072 |