MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis.
International audience BACKGROUND: Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and sp...
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ftuniparissaclay:oai:HAL:hal-00408931v1 2023-05-15T15:59:54+02:00 MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. Maquart, Marianne Le Flèche, Philippe Foster, Geoffrey Tryland, Morten Ramisse, Françoise Djønne, Berit Al Dahouk, Sascha Jacques, Isabelle Neubauer, Heinrich Walravens, Karl Godfroid, Jacques Cloeckaert, Axel Vergnaud, Gilles Institut de génétique et microbiologie Orsay (IGM) Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) French "Delegation Generale pour l'Armement" (DGA), European Defense Agency 2009 https://hal.science/hal-00408931 https://hal.science/hal-00408931/document https://hal.science/hal-00408931/file/2009_Macquart_BMC-Microbiology_1.pdf https://doi.org/10.1186/1471-2180-9-145 en eng HAL CCSD BioMed Central info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-9-145 info:eu-repo/semantics/altIdentifier/pmid/19619320 hal-00408931 https://hal.science/hal-00408931 https://hal.science/hal-00408931/document https://hal.science/hal-00408931/file/2009_Macquart_BMC-Microbiology_1.pdf doi:10.1186/1471-2180-9-145 PRODINRA: 147425 PUBMED: 19619320 WOS: 000268669100002 info:eu-repo/semantics/OpenAccess ISSN: 1471-2180 BMC Microbiology https://hal.science/hal-00408931 BMC Microbiology, 2009, 9, pp.145. ⟨10.1186/1471-2180-9-145⟩ [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2009 ftuniparissaclay https://doi.org/10.1186/1471-2180-9-145 2023-04-03T09:46:12Z International audience BACKGROUND: Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used. RESULTS: 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals (Cystophora cristata) and the two others comprising other seal species isolates. CONCLUSION: The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with ... Article in Journal/Newspaper Cystophora cristata minke whale Archives ouvertes de Paris-Saclay New Zealand BMC Microbiology 9 1 145 |
institution |
Open Polar |
collection |
Archives ouvertes de Paris-Saclay |
op_collection_id |
ftuniparissaclay |
language |
English |
topic |
[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Maquart, Marianne Le Flèche, Philippe Foster, Geoffrey Tryland, Morten Ramisse, Françoise Djønne, Berit Al Dahouk, Sascha Jacques, Isabelle Neubauer, Heinrich Walravens, Karl Godfroid, Jacques Cloeckaert, Axel Vergnaud, Gilles MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. |
topic_facet |
[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
International audience BACKGROUND: Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used. RESULTS: 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals (Cystophora cristata) and the two others comprising other seal species isolates. CONCLUSION: The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with ... |
author2 |
Institut de génétique et microbiologie Orsay (IGM) Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) French "Delegation Generale pour l'Armement" (DGA), European Defense Agency |
format |
Article in Journal/Newspaper |
author |
Maquart, Marianne Le Flèche, Philippe Foster, Geoffrey Tryland, Morten Ramisse, Françoise Djønne, Berit Al Dahouk, Sascha Jacques, Isabelle Neubauer, Heinrich Walravens, Karl Godfroid, Jacques Cloeckaert, Axel Vergnaud, Gilles |
author_facet |
Maquart, Marianne Le Flèche, Philippe Foster, Geoffrey Tryland, Morten Ramisse, Françoise Djønne, Berit Al Dahouk, Sascha Jacques, Isabelle Neubauer, Heinrich Walravens, Karl Godfroid, Jacques Cloeckaert, Axel Vergnaud, Gilles |
author_sort |
Maquart, Marianne |
title |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. |
title_short |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. |
title_full |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. |
title_fullStr |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. |
title_full_unstemmed |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. |
title_sort |
mlva-16 typing of 295 marine mammal brucella isolates from different animal and geographic origins identifies 7 major groups within brucella ceti and brucella pinnipedialis. |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.science/hal-00408931 https://hal.science/hal-00408931/document https://hal.science/hal-00408931/file/2009_Macquart_BMC-Microbiology_1.pdf https://doi.org/10.1186/1471-2180-9-145 |
geographic |
New Zealand |
geographic_facet |
New Zealand |
genre |
Cystophora cristata minke whale |
genre_facet |
Cystophora cristata minke whale |
op_source |
ISSN: 1471-2180 BMC Microbiology https://hal.science/hal-00408931 BMC Microbiology, 2009, 9, pp.145. ⟨10.1186/1471-2180-9-145⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-9-145 info:eu-repo/semantics/altIdentifier/pmid/19619320 hal-00408931 https://hal.science/hal-00408931 https://hal.science/hal-00408931/document https://hal.science/hal-00408931/file/2009_Macquart_BMC-Microbiology_1.pdf doi:10.1186/1471-2180-9-145 PRODINRA: 147425 PUBMED: 19619320 WOS: 000268669100002 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/1471-2180-9-145 |
container_title |
BMC Microbiology |
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9 |
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1 |
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145 |
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1766395796095238144 |