Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax).
Although fish farming has been practiced for 4,000 years, aquaculture research dates back only to about 1,870, whereas molecular techniques, in addition to the more traditional methods of biotechnology, were introduced only recently. Contemporary genomic approaches (often adapted from human or medic...
Published in: | Reviews in Fish Biology and Fisheries |
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Online Access: | http://hdl.handle.net/11383/1780316 https://doi.org/10.1007/s11160-012-9290-6 |
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ftuninsubriairis:oai:irinsubria.uninsubria.it:11383/1780316 2024-04-14T08:09:18+00:00 Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax). TEROVA, GENCIANA RIMOLDI, SIMONA BERNARDINI, GIOVANNI BATTISTA Parisi G Gasco L Pais A Terova, Genciana Rimoldi, Simona Parisi, G Gasco, L Pais, A Bernardini, GIOVANNI BATTISTA 2013 ELETTRONICO http://hdl.handle.net/11383/1780316 https://doi.org/10.1007/s11160-012-9290-6 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000318494000003 volume:23 firstpage:175 lastpage:194 numberofpages:20 journal:REVIEWS IN FISH BIOLOGY AND FISHERIES http://hdl.handle.net/11383/1780316 doi:10.1007/s11160-012-9290-6 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84877061014 Aquaculture biotechnology Functional genomics Hypoxic stress biomarkers Hypercapnia Molecular cloning Gene expression Fish innate immune system TROUT ONCORHYNCHUS-MYKISS GLUCOSE TRANSPORTERS GLUT PUFFERFISH FUGU RUBRIPES EST-BASED IDENTIFICATION MESSENGER-RNA LEVELS COD GADUS-MORHUA C-FOS ANTIMICROBIAL PEPTIDE RAINBOW-TROUT NA+/H+ EXCHANGER info:eu-repo/semantics/article 2013 ftuninsubriairis https://doi.org/10.1007/s11160-012-9290-6 2024-03-21T19:01:29Z Although fish farming has been practiced for 4,000 years, aquaculture research dates back only to about 1,870, whereas molecular techniques, in addition to the more traditional methods of biotechnology, were introduced only recently. Contemporary genomic approaches (often adapted from human or medical research) such as cDNA cloning and sequencing, cDNA microarray/expression analysis, and functional genomics, combined with improvements in transgenic technologies, have enhanced possibilities for aquacultural biotechnologists for improving fish growth rates and increasing resistance to pathogens and stressors. Although genomic technologies in fish have been applied primarily to model organisms, such as zebrafish (Danio rerio), fugu or pufferfish (Tetraodon nigroviridis), and medaka (Oryzias latipes), many teleosts of interest for biological research and with potential application in aquaculture have unique physiological characteristics that cannot be directly investigated from the study of small laboratory fish models. As a consequence, large-scale genomic research studies are increasingly being applied to farmed species of economic relevance, such as farmed rainbow trout, Atlantic salmon, tilapia, catfish, and sea bass. Accordingly, we describe the utilization of molecular cloning and gene expression analysis in cultured European sea bass (Dicentrarchus labrax) to generate "transcriptome-focused" information which may enable a better understanding of the transcriptional programs that underlie fish respiratory physiology and immune response pathways. Article in Journal/Newspaper Atlantic salmon Gadus morhua IRInSubria - Institutional Repository Insubria (Università degli Studi dell’Insubria) Reviews in Fish Biology and Fisheries 23 2 175 194 |
institution |
Open Polar |
collection |
IRInSubria - Institutional Repository Insubria (Università degli Studi dell’Insubria) |
op_collection_id |
ftuninsubriairis |
language |
English |
topic |
Aquaculture biotechnology Functional genomics Hypoxic stress biomarkers Hypercapnia Molecular cloning Gene expression Fish innate immune system TROUT ONCORHYNCHUS-MYKISS GLUCOSE TRANSPORTERS GLUT PUFFERFISH FUGU RUBRIPES EST-BASED IDENTIFICATION MESSENGER-RNA LEVELS COD GADUS-MORHUA C-FOS ANTIMICROBIAL PEPTIDE RAINBOW-TROUT NA+/H+ EXCHANGER |
spellingShingle |
Aquaculture biotechnology Functional genomics Hypoxic stress biomarkers Hypercapnia Molecular cloning Gene expression Fish innate immune system TROUT ONCORHYNCHUS-MYKISS GLUCOSE TRANSPORTERS GLUT PUFFERFISH FUGU RUBRIPES EST-BASED IDENTIFICATION MESSENGER-RNA LEVELS COD GADUS-MORHUA C-FOS ANTIMICROBIAL PEPTIDE RAINBOW-TROUT NA+/H+ EXCHANGER TEROVA, GENCIANA RIMOLDI, SIMONA BERNARDINI, GIOVANNI BATTISTA Parisi G Gasco L Pais A Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax). |
topic_facet |
Aquaculture biotechnology Functional genomics Hypoxic stress biomarkers Hypercapnia Molecular cloning Gene expression Fish innate immune system TROUT ONCORHYNCHUS-MYKISS GLUCOSE TRANSPORTERS GLUT PUFFERFISH FUGU RUBRIPES EST-BASED IDENTIFICATION MESSENGER-RNA LEVELS COD GADUS-MORHUA C-FOS ANTIMICROBIAL PEPTIDE RAINBOW-TROUT NA+/H+ EXCHANGER |
description |
Although fish farming has been practiced for 4,000 years, aquaculture research dates back only to about 1,870, whereas molecular techniques, in addition to the more traditional methods of biotechnology, were introduced only recently. Contemporary genomic approaches (often adapted from human or medical research) such as cDNA cloning and sequencing, cDNA microarray/expression analysis, and functional genomics, combined with improvements in transgenic technologies, have enhanced possibilities for aquacultural biotechnologists for improving fish growth rates and increasing resistance to pathogens and stressors. Although genomic technologies in fish have been applied primarily to model organisms, such as zebrafish (Danio rerio), fugu or pufferfish (Tetraodon nigroviridis), and medaka (Oryzias latipes), many teleosts of interest for biological research and with potential application in aquaculture have unique physiological characteristics that cannot be directly investigated from the study of small laboratory fish models. As a consequence, large-scale genomic research studies are increasingly being applied to farmed species of economic relevance, such as farmed rainbow trout, Atlantic salmon, tilapia, catfish, and sea bass. Accordingly, we describe the utilization of molecular cloning and gene expression analysis in cultured European sea bass (Dicentrarchus labrax) to generate "transcriptome-focused" information which may enable a better understanding of the transcriptional programs that underlie fish respiratory physiology and immune response pathways. |
author2 |
Terova, Genciana Rimoldi, Simona Parisi, G Gasco, L Pais, A Bernardini, GIOVANNI BATTISTA |
format |
Article in Journal/Newspaper |
author |
TEROVA, GENCIANA RIMOLDI, SIMONA BERNARDINI, GIOVANNI BATTISTA Parisi G Gasco L Pais A |
author_facet |
TEROVA, GENCIANA RIMOLDI, SIMONA BERNARDINI, GIOVANNI BATTISTA Parisi G Gasco L Pais A |
author_sort |
TEROVA, GENCIANA |
title |
Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax). |
title_short |
Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax). |
title_full |
Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax). |
title_fullStr |
Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax). |
title_full_unstemmed |
Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax). |
title_sort |
molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in european sea bass (dicentrarchus labrax). |
publishDate |
2013 |
url |
http://hdl.handle.net/11383/1780316 https://doi.org/10.1007/s11160-012-9290-6 |
genre |
Atlantic salmon Gadus morhua |
genre_facet |
Atlantic salmon Gadus morhua |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000318494000003 volume:23 firstpage:175 lastpage:194 numberofpages:20 journal:REVIEWS IN FISH BIOLOGY AND FISHERIES http://hdl.handle.net/11383/1780316 doi:10.1007/s11160-012-9290-6 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84877061014 |
op_doi |
https://doi.org/10.1007/s11160-012-9290-6 |
container_title |
Reviews in Fish Biology and Fisheries |
container_volume |
23 |
container_issue |
2 |
container_start_page |
175 |
op_container_end_page |
194 |
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1796306793781002240 |