Extracellular enzymes produced by bacteria isolated from mrine invertbrates
In order to select marine bacteria with the ability to produce extracellular enzymes (EEC), samples from Argopecten purpuratus and Crassostrea gigas in cultivation as well as from other interticlal and benthic invertebrates were analyzed. The selection of producer EEC strains was carried out in Mari...
Published in: | Revista Peruana de Biología |
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Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
2000
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Online Access: | https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/6828 https://doi.org/10.15381/rpb.v7i2.6828 |
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ftuninmsanmarcos:oai:ojs.csi.unmsm:article/6828 2023-09-05T13:18:59+02:00 Extracellular enzymes produced by bacteria isolated from mrine invertbrates Producción de enzimas extracelulares por bacterias aisladas de invertebrados marinos León, Jorge Pellón, Fabiola Unda, Verónica David, Janet Anaya, César Mendoza, Victoria 2000-12-29 application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/6828 https://doi.org/10.15381/rpb.v7i2.6828 spa spa Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/6828/6043 https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/6828 doi:10.15381/rpb.v7i2.6828 Derechos de autor 2000 Jorge León, Fabiola Pellón, Verónica Unda, Janet David, César Anaya, Victoria Mendoza https://creativecommons.org/licenses/by-nc-sa/4.0 Revista Peruana de Biología; Vol. 7 No. 2 (2000); 202 - 210 Revista Peruana de Biología; Vol. 7 Núm. 2 (2000); 202 - 210 1727-9933 1561-0837 extracellular enzymes metabolites marine bacteria invertebrate marine biotechnology enzimas extracelulares metabolitos bacterias marinas invertebrados biotecnología marina info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2000 ftuninmsanmarcos https://doi.org/10.15381/rpb.v7i2.6828 2023-08-22T09:35:55Z In order to select marine bacteria with the ability to produce extracellular enzymes (EEC), samples from Argopecten purpuratus and Crassostrea gigas in cultivation as well as from other interticlal and benthic invertebrates were analyzed. The selection of producer EEC strains was carried out in Marine Agar (MA) with addition of the relevant substratum (starch, casein, tween-80, lecithine, DNA and gelatine). The EEC producer strains evaluation was carried out on 102 isolates. The results show that bacteria associated toA. purpuratusand C. gigashave the best multienzymatic activities; however, Semimytilus algosus, Tetrapigus niger and Thais chocolata are also important sources of bacteria producers of EEC. The qualitative multienzymatic activity in MA (clear or opaque zones around the colonies in mm of diameter) varied from 6 to > 16 mm. The frequency of multienzymatic production was caseinase 62,74%, tween-esterase 57,84%, amylase 52,94%, gelatinase 38,23%, DNAse 33,3%, agarase 5,43% and lecithinase 90,0%. The results suggest the possibility of using native strains for biotechnological purposes. Con el fin de seleccionar las bacterias marinas con la capacidad de producir enzimas extracelulares ( CEE ) , se analizaron muestras de Argopecten purpuratus y Crassostrea gigas en el cultivo , así como de otros invertebrados interticlal y bentónicos. La selección de cepas de la CEE de productores se llevó a cabo en Agar Marino ( MA ) con la adición del sustrato relevante ( almidón , caseína , Tween - 80 , lecitina , ADN y gelatina ) . La CEE evaluación cepas productoras se llevó a cabo en 102 aislamientos . Los resultados muestran que las bacterias asociadas TOA . purpuratusand C. gigashave las mejores actividades multienzimáticos Sin embargo , Semimytilus algosus, Tetrapigus niger y Thais chocolata también son fuentes importantes de productores de bacterias de la CEE. La actividad multienzimático cualitativa en MA ( zonas claras u opacas alrededor de las colonias en mm de diámetro ) varió de 6 a > 16 mm . La ... Article in Journal/Newspaper Crassostrea gigas Universidad Nacional Mayor de San Marcos: Revistas de investigación UNMSM Revista Peruana de Biología 7 2 |
institution |
Open Polar |
collection |
Universidad Nacional Mayor de San Marcos: Revistas de investigación UNMSM |
op_collection_id |
ftuninmsanmarcos |
language |
Spanish |
topic |
extracellular enzymes metabolites marine bacteria invertebrate marine biotechnology enzimas extracelulares metabolitos bacterias marinas invertebrados biotecnología marina |
spellingShingle |
extracellular enzymes metabolites marine bacteria invertebrate marine biotechnology enzimas extracelulares metabolitos bacterias marinas invertebrados biotecnología marina León, Jorge Pellón, Fabiola Unda, Verónica David, Janet Anaya, César Mendoza, Victoria Extracellular enzymes produced by bacteria isolated from mrine invertbrates |
topic_facet |
extracellular enzymes metabolites marine bacteria invertebrate marine biotechnology enzimas extracelulares metabolitos bacterias marinas invertebrados biotecnología marina |
description |
In order to select marine bacteria with the ability to produce extracellular enzymes (EEC), samples from Argopecten purpuratus and Crassostrea gigas in cultivation as well as from other interticlal and benthic invertebrates were analyzed. The selection of producer EEC strains was carried out in Marine Agar (MA) with addition of the relevant substratum (starch, casein, tween-80, lecithine, DNA and gelatine). The EEC producer strains evaluation was carried out on 102 isolates. The results show that bacteria associated toA. purpuratusand C. gigashave the best multienzymatic activities; however, Semimytilus algosus, Tetrapigus niger and Thais chocolata are also important sources of bacteria producers of EEC. The qualitative multienzymatic activity in MA (clear or opaque zones around the colonies in mm of diameter) varied from 6 to > 16 mm. The frequency of multienzymatic production was caseinase 62,74%, tween-esterase 57,84%, amylase 52,94%, gelatinase 38,23%, DNAse 33,3%, agarase 5,43% and lecithinase 90,0%. The results suggest the possibility of using native strains for biotechnological purposes. Con el fin de seleccionar las bacterias marinas con la capacidad de producir enzimas extracelulares ( CEE ) , se analizaron muestras de Argopecten purpuratus y Crassostrea gigas en el cultivo , así como de otros invertebrados interticlal y bentónicos. La selección de cepas de la CEE de productores se llevó a cabo en Agar Marino ( MA ) con la adición del sustrato relevante ( almidón , caseína , Tween - 80 , lecitina , ADN y gelatina ) . La CEE evaluación cepas productoras se llevó a cabo en 102 aislamientos . Los resultados muestran que las bacterias asociadas TOA . purpuratusand C. gigashave las mejores actividades multienzimáticos Sin embargo , Semimytilus algosus, Tetrapigus niger y Thais chocolata también son fuentes importantes de productores de bacterias de la CEE. La actividad multienzimático cualitativa en MA ( zonas claras u opacas alrededor de las colonias en mm de diámetro ) varió de 6 a > 16 mm . La ... |
format |
Article in Journal/Newspaper |
author |
León, Jorge Pellón, Fabiola Unda, Verónica David, Janet Anaya, César Mendoza, Victoria |
author_facet |
León, Jorge Pellón, Fabiola Unda, Verónica David, Janet Anaya, César Mendoza, Victoria |
author_sort |
León, Jorge |
title |
Extracellular enzymes produced by bacteria isolated from mrine invertbrates |
title_short |
Extracellular enzymes produced by bacteria isolated from mrine invertbrates |
title_full |
Extracellular enzymes produced by bacteria isolated from mrine invertbrates |
title_fullStr |
Extracellular enzymes produced by bacteria isolated from mrine invertbrates |
title_full_unstemmed |
Extracellular enzymes produced by bacteria isolated from mrine invertbrates |
title_sort |
extracellular enzymes produced by bacteria isolated from mrine invertbrates |
publisher |
Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas |
publishDate |
2000 |
url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/6828 https://doi.org/10.15381/rpb.v7i2.6828 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Revista Peruana de Biología; Vol. 7 No. 2 (2000); 202 - 210 Revista Peruana de Biología; Vol. 7 Núm. 2 (2000); 202 - 210 1727-9933 1561-0837 |
op_relation |
https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/6828/6043 https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/6828 doi:10.15381/rpb.v7i2.6828 |
op_rights |
Derechos de autor 2000 Jorge León, Fabiola Pellón, Verónica Unda, Janet David, César Anaya, Victoria Mendoza https://creativecommons.org/licenses/by-nc-sa/4.0 |
op_doi |
https://doi.org/10.15381/rpb.v7i2.6828 |
container_title |
Revista Peruana de Biología |
container_volume |
7 |
container_issue |
2 |
_version_ |
1776199822938734592 |