Isolation of serovars of Salmonella enterica from feces of domestic and wild animals in captivity

• Published in: Analecta Veterinaria
• Main Authors: Pidone, Claudio Luis, Zerpa, Corina, Caffer, María Inés, Moroni, Mirian, Comba, Eduardo Rubén
• Format: Article in Journal/Newspaper
• Language: Spanish
• Published: Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata 2018
• Subjects: Salmonella; zoológico; serovariedades; zoonosis; zoo; serovars; zoonoses; Argentina; oso polar; polar bear
• Online Access: https://revistas.unlp.edu.ar/analecta/article/view/5165; https://doi.org/10.24215/15142590e028

Fecal samples corresponding to 27 species of animals in captivity in the Mendoza Zoo (Argentina) were collected, in order to find asymptomatic carriers of Salmonella. The fecal samples obtained with swabs were seeded in Müller-Kauffmann base tetrathionate and incubated at 43 °C in a water bath for 24 h. Then, aliquots of this medium were seeded on xylose-lysine-deoxycholate agar and, finally, formed colonies were identified using conventional biochemical tests. As a result, Salmonella serovar Meleagridis was isolated from a polar bear and Salmonella serovar Give from a brown bear and a carayá monkey samples. Both serovars are associated with cases of salmonellosis in humans. Se recolectaron muestras de materia fecal correspondientes a 27 especies de animales en cautiverio pertenecientes al Jardín Zoológico de Mendoza (Argentina), con el fin de encontrar portadores asintomáticos de Salmonella. Las muestras de materia fecal obtenidas con hisopos se sembraron en caldo tetrationato base de Müller-Kauffmann y se incubaron a 43 ºC en baño María durante 24 h. Alícuotas de este medio se sembraron en agar xilosa-lisina-desoxicolato y, finalmente, las colonias desarrolladas se identificaron utilizando pruebas bioquímicas convencionales. Como resultado, se aisló Salmonella serovar Meleagridis a partir de muestras de un oso polar y Salmonella serovar Give a partir de las de un oso pardo y un mono carayá. Ambas serovariedades están asociadas con casos de salmonelosis en humanos.