Is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress?
An evolutionary-conserved family of enzymes known as the matrix metalloproteinases (MMPs) play major roles in tissue remodeling. While much is known about MMPs in mammalian vertebrates, their presence and function in non-mammalian vertebrates is not well studied. In teleosts, MMPs have been shown to...
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ftuninhampshire:oai:scholars.unh.edu:urc-1398 2023-06-11T04:17:41+02:00 Is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress? Faircloth, Elizabeth 2017-05-01T07:00:00Z application/pdf https://scholars.unh.edu/urc/394 https://scholars.unh.edu/context/urc/article/1398/viewcontent/URC_Poster_draft_5_Faircloth.pdf unknown University of New Hampshire Scholars' Repository https://scholars.unh.edu/urc/394 https://scholars.unh.edu/context/urc/article/1398/viewcontent/URC_Poster_draft_5_Faircloth.pdf Document References (1) Detection of Functional Matrix Metalloproteinases by Zymography Xueyou Hu1, Christine Beeton1 1Department of Molecular Physiology and Biophysics, Baylor College of Medicine (2) Kubota, Satoshi, et al. "Occurrence of two distinct types of tissue inhibitor of metalloproteinases-2 in teleost fish." Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression 1629.1 (2003): 102-108. (3) Nagase, Hideaki, Robert Visse, and Gillian Murphy. "Structure and function of matrix metalloproteinases and TIMPs." Cardiovascular research 69.3 (2006): 562-573. Image References https://ghsmp.org/fishery-economics-sustainable-fish-consumption-and-banning-industrial-fishing-article/ (fishing trawler) http://www.greenpeace.org/international/Global/international/planet-2/image/2008/5/hooks-and-lines.jpg hook and line http://www.greenpeace.org/international/Global/international/planet-2/image/2008/5/beam-trawling.jpg bottom trawl http://2014.igem.org/Team:WPI-Worcester/Overview (Plasma pre-edit) https://cellularphysiology.wikispaces.com/Gel+Electrophoresis gel electrophoresis Gong, Yixuan, Uma D. Chippada-Venkata, and William K. Oh. "Roles of matrix metalloproteinases and their natural inhibitors in prostate cancer progression." Cancers 6.3 (2014): 1298-1327. MMP Image Undergraduate Research Conference (URC) Student Presentations Gelatin Zymography Squalus acanthias Dogfish Aquaculture and Fisheries Biology Structural Biology text 2017 ftuninhampshire 2023-05-04T17:35:27Z An evolutionary-conserved family of enzymes known as the matrix metalloproteinases (MMPs) play major roles in tissue remodeling. While much is known about MMPs in mammalian vertebrates, their presence and function in non-mammalian vertebrates is not well studied. In teleosts, MMPs have been shown to degrade skeletal muscle and thus, may affect the quality of fillet at market. Standard commercial fishing practices can be stressful, which could also adversely affect fillet quality. Interestingly we observed that MMPs are expressed in skeletal muscle of the spiny dogfish, Squalus acanthias. Therefore, we hypothesized that skeletal muscle MMP expression in dogfish is correlated to capture method. Skeletal muscles were obtained from dogfish caught under high stress (24-hr tow) and low stress (hook and line) conditions. Dogfish skeletal muscle proteins were extracted and subjected to gelatin zymography to identify MMPs. Among them, a few were deferentially expressed in dogfish caught using high and low stress methods. These results may have implications for commercial fishing practices that ultimately provide a high-quality product to consumers. Text spiny dogfish Squalus acanthias University of New Hampshire: Scholars Repository |
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University of New Hampshire: Scholars Repository |
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Gelatin Zymography Squalus acanthias Dogfish Aquaculture and Fisheries Biology Structural Biology |
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Gelatin Zymography Squalus acanthias Dogfish Aquaculture and Fisheries Biology Structural Biology Faircloth, Elizabeth Is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress? |
topic_facet |
Gelatin Zymography Squalus acanthias Dogfish Aquaculture and Fisheries Biology Structural Biology |
description |
An evolutionary-conserved family of enzymes known as the matrix metalloproteinases (MMPs) play major roles in tissue remodeling. While much is known about MMPs in mammalian vertebrates, their presence and function in non-mammalian vertebrates is not well studied. In teleosts, MMPs have been shown to degrade skeletal muscle and thus, may affect the quality of fillet at market. Standard commercial fishing practices can be stressful, which could also adversely affect fillet quality. Interestingly we observed that MMPs are expressed in skeletal muscle of the spiny dogfish, Squalus acanthias. Therefore, we hypothesized that skeletal muscle MMP expression in dogfish is correlated to capture method. Skeletal muscles were obtained from dogfish caught under high stress (24-hr tow) and low stress (hook and line) conditions. Dogfish skeletal muscle proteins were extracted and subjected to gelatin zymography to identify MMPs. Among them, a few were deferentially expressed in dogfish caught using high and low stress methods. These results may have implications for commercial fishing practices that ultimately provide a high-quality product to consumers. |
format |
Text |
author |
Faircloth, Elizabeth |
author_facet |
Faircloth, Elizabeth |
author_sort |
Faircloth, Elizabeth |
title |
Is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress? |
title_short |
Is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress? |
title_full |
Is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress? |
title_fullStr |
Is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress? |
title_full_unstemmed |
Is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress? |
title_sort |
is the expression of tissue-degrading enzymes in the skeletal muscle of spiny dogfish correlated to capture stress? |
publisher |
University of New Hampshire Scholars' Repository |
publishDate |
2017 |
url |
https://scholars.unh.edu/urc/394 https://scholars.unh.edu/context/urc/article/1398/viewcontent/URC_Poster_draft_5_Faircloth.pdf |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_source |
Undergraduate Research Conference (URC) Student Presentations |
op_relation |
https://scholars.unh.edu/urc/394 https://scholars.unh.edu/context/urc/article/1398/viewcontent/URC_Poster_draft_5_Faircloth.pdf |
op_rights |
Document References (1) Detection of Functional Matrix Metalloproteinases by Zymography Xueyou Hu1, Christine Beeton1 1Department of Molecular Physiology and Biophysics, Baylor College of Medicine (2) Kubota, Satoshi, et al. "Occurrence of two distinct types of tissue inhibitor of metalloproteinases-2 in teleost fish." Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression 1629.1 (2003): 102-108. (3) Nagase, Hideaki, Robert Visse, and Gillian Murphy. "Structure and function of matrix metalloproteinases and TIMPs." Cardiovascular research 69.3 (2006): 562-573. Image References https://ghsmp.org/fishery-economics-sustainable-fish-consumption-and-banning-industrial-fishing-article/ (fishing trawler) http://www.greenpeace.org/international/Global/international/planet-2/image/2008/5/hooks-and-lines.jpg hook and line http://www.greenpeace.org/international/Global/international/planet-2/image/2008/5/beam-trawling.jpg bottom trawl http://2014.igem.org/Team:WPI-Worcester/Overview (Plasma pre-edit) https://cellularphysiology.wikispaces.com/Gel+Electrophoresis gel electrophoresis Gong, Yixuan, Uma D. Chippada-Venkata, and William K. Oh. "Roles of matrix metalloproteinases and their natural inhibitors in prostate cancer progression." Cancers 6.3 (2014): 1298-1327. MMP Image |
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