Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater
Marinobacter sp. W1-16 from Antarctic surface seawater was analysed for the production of extracellular polymeric substances (EPSs). Enhancement of the EPS biosynthesis was carried out by evaluating the influences of the carbon source (type and concentration), temperature, pH and salinity. EPS yield...
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Online Access: | http://hdl.handle.net/11367/74090 https://doi.org/10.1017/S0954102018000482 |
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ftuninapoliparth:oai:ricerca.uniparthenope.it:11367/74090 2024-04-14T08:01:39+00:00 Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Finore, Ilaria Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Finore, Ilaria Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina 2019 http://hdl.handle.net/11367/74090 https://doi.org/10.1017/S0954102018000482 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000463086400004 firstpage:1 lastpage:11 numberofpages:11 journal:ANTARCTIC SCIENCE http://hdl.handle.net/11367/74090 doi:10.1017/S0954102018000482 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85063129967 info:eu-repo/semantics/article 2019 ftuninapoliparth https://doi.org/10.1017/S0954102018000482 2024-03-21T18:01:58Z Marinobacter sp. W1-16 from Antarctic surface seawater was analysed for the production of extracellular polymeric substances (EPSs). Enhancement of the EPS biosynthesis was carried out by evaluating the influences of the carbon source (type and concentration), temperature, pH and salinity. EPS yields varied strongly depending on sugar substrate and temperature, while pH and salinity did not strongly affect levels of EPS production. Marinobacter sp. W1-16 produced the highest quantity of EPSs when growing at 15°C and pH 8, in the presence of 2% glucose and 3% NaCl. The EPS chemical characterization revealed a molecular weight of about 260 kDa. Colorimetric assays determined a higher quantity of carbohydrate than of proteins and uronic acids, as well as the presence of sulphate, in the extracted EPSs. The monosaccharidic composition resulted in Glc:Man:Gal:GalN:GalA:GlcA in relative molar proportions of 1:0.9:0.2:0.1:0.1:0.01. Some biotechnological potentialities (i.e. emulsifying and cryoprotective actions, and heavy metal binding properties) of the EPSs were proved, suggesting possible industrial and bioremediation applications. Article in Journal/Newspaper Antarc* Antarctic Università degli Studi di Napoli "Parthenope": CINECA IRIS Antarctic Antarctic Science 31 2 69 79 |
institution |
Open Polar |
collection |
Università degli Studi di Napoli "Parthenope": CINECA IRIS |
op_collection_id |
ftuninapoliparth |
language |
English |
description |
Marinobacter sp. W1-16 from Antarctic surface seawater was analysed for the production of extracellular polymeric substances (EPSs). Enhancement of the EPS biosynthesis was carried out by evaluating the influences of the carbon source (type and concentration), temperature, pH and salinity. EPS yields varied strongly depending on sugar substrate and temperature, while pH and salinity did not strongly affect levels of EPS production. Marinobacter sp. W1-16 produced the highest quantity of EPSs when growing at 15°C and pH 8, in the presence of 2% glucose and 3% NaCl. The EPS chemical characterization revealed a molecular weight of about 260 kDa. Colorimetric assays determined a higher quantity of carbohydrate than of proteins and uronic acids, as well as the presence of sulphate, in the extracted EPSs. The monosaccharidic composition resulted in Glc:Man:Gal:GalN:GalA:GlcA in relative molar proportions of 1:0.9:0.2:0.1:0.1:0.01. Some biotechnological potentialities (i.e. emulsifying and cryoprotective actions, and heavy metal binding properties) of the EPSs were proved, suggesting possible industrial and bioremediation applications. |
author2 |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Finore, Ilaria Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina |
format |
Article in Journal/Newspaper |
author |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Finore, Ilaria Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina |
spellingShingle |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Finore, Ilaria Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater |
author_facet |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Finore, Ilaria Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina |
author_sort |
Caruso, Consolazione |
title |
Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater |
title_short |
Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater |
title_full |
Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater |
title_fullStr |
Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater |
title_full_unstemmed |
Isolation, characterization and optimization of EPSs produced by a cold-adapted Marinobacter isolate from Antarctic seawater |
title_sort |
isolation, characterization and optimization of epss produced by a cold-adapted marinobacter isolate from antarctic seawater |
publishDate |
2019 |
url |
http://hdl.handle.net/11367/74090 https://doi.org/10.1017/S0954102018000482 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000463086400004 firstpage:1 lastpage:11 numberofpages:11 journal:ANTARCTIC SCIENCE http://hdl.handle.net/11367/74090 doi:10.1017/S0954102018000482 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85063129967 |
op_doi |
https://doi.org/10.1017/S0954102018000482 |
container_title |
Antarctic Science |
container_volume |
31 |
container_issue |
2 |
container_start_page |
69 |
op_container_end_page |
79 |
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1796310081408598016 |