Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater
The EPS-producing Pseudoalteromonas sp. MER144 was selected among 606 isolates from Antarctic seawater due to its evident slimy appearance on agar plates. The production of EPSs was enhanced by a step-by-step approach varying the carbon source, substrate and NaCl concentrations, temperature, and pH....
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Online Access: | http://hdl.handle.net/11367/64489 https://doi.org/10.1007/s11356-017-0851-z https://link.springer.com/article/10.1007%2Fs11356-017-0851-z |
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ftuninapoliparth:oai:ricerca.uniparthenope.it:11367/64489 2024-04-21T07:51:12+00:00 Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina 2018 http://hdl.handle.net/11367/64489 https://doi.org/10.1007/s11356-017-0851-z https://link.springer.com/article/10.1007%2Fs11356-017-0851-z eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000425770300059 volume:25 issue:5 firstpage:4667 lastpage:4677 numberofpages:11 journal:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL http://hdl.handle.net/11367/64489 doi:10.1007/s11356-017-0851-z info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85035796043 https://link.springer.com/article/10.1007%2Fs11356-017-0851-z Antarctica Cryoprotection Exopolymer Heavy metal Environmental Chemistry Pollution Health Toxicology and Mutagenesis info:eu-repo/semantics/article 2018 ftuninapoliparth https://doi.org/10.1007/s11356-017-0851-z 2024-03-28T01:12:57Z The EPS-producing Pseudoalteromonas sp. MER144 was selected among 606 isolates from Antarctic seawater due to its evident slimy appearance on agar plates. The production of EPSs was enhanced by a step-by-step approach varying the carbon source, substrate and NaCl concentrations, temperature, and pH. Optimal conditions for the EPS production resulted at temperature of 4 °C and pH 7, with addition of 2% sucrose (w/v) and 3% NaCl (w/v). EPSs produced under optimal conditions were chemically characterized, resulting in a moderate carbohydrate content (35%), uronic acids (14%), and proteins (12%). Monosaccharide composition was estimated to be Glu:Man:GluN:Ara:GluA:GalA:Gal (1:0.36:0.26:0.06:0.06:0.05:0.03), while the estimated molecular weight was about 250 kDa. The addition of sucrose in the culture medium, by stimulating the EPS production, allowed MER144 to tolerate higher concentrations of mercury and cadmium. This finding was probably dependent on the presence of uronic acids and sulfate groups, which can bind cations, in the extracted EPSs. Monitoring EPS production under optimal conditions at different concentrations of mercury and cadmium revealed that EPS amounts increased at increasing heavy metal concentrations, indicating an adaptation to the stress conditions tested. Article in Journal/Newspaper Antarc* Antarctic Antarctica Università degli Studi di Napoli "Parthenope": CINECA IRIS Environmental Science and Pollution Research 25 5 4667 4677 |
institution |
Open Polar |
collection |
Università degli Studi di Napoli "Parthenope": CINECA IRIS |
op_collection_id |
ftuninapoliparth |
language |
English |
topic |
Antarctica Cryoprotection Exopolymer Heavy metal Environmental Chemistry Pollution Health Toxicology and Mutagenesis |
spellingShingle |
Antarctica Cryoprotection Exopolymer Heavy metal Environmental Chemistry Pollution Health Toxicology and Mutagenesis Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater |
topic_facet |
Antarctica Cryoprotection Exopolymer Heavy metal Environmental Chemistry Pollution Health Toxicology and Mutagenesis |
description |
The EPS-producing Pseudoalteromonas sp. MER144 was selected among 606 isolates from Antarctic seawater due to its evident slimy appearance on agar plates. The production of EPSs was enhanced by a step-by-step approach varying the carbon source, substrate and NaCl concentrations, temperature, and pH. Optimal conditions for the EPS production resulted at temperature of 4 °C and pH 7, with addition of 2% sucrose (w/v) and 3% NaCl (w/v). EPSs produced under optimal conditions were chemically characterized, resulting in a moderate carbohydrate content (35%), uronic acids (14%), and proteins (12%). Monosaccharide composition was estimated to be Glu:Man:GluN:Ara:GluA:GalA:Gal (1:0.36:0.26:0.06:0.06:0.05:0.03), while the estimated molecular weight was about 250 kDa. The addition of sucrose in the culture medium, by stimulating the EPS production, allowed MER144 to tolerate higher concentrations of mercury and cadmium. This finding was probably dependent on the presence of uronic acids and sulfate groups, which can bind cations, in the extracted EPSs. Monitoring EPS production under optimal conditions at different concentrations of mercury and cadmium revealed that EPS amounts increased at increasing heavy metal concentrations, indicating an adaptation to the stress conditions tested. |
author2 |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina |
format |
Article in Journal/Newspaper |
author |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina |
author_facet |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina |
author_sort |
Caruso, Consolazione |
title |
Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater |
title_short |
Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater |
title_full |
Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater |
title_fullStr |
Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater |
title_full_unstemmed |
Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater |
title_sort |
extracellular polymeric substances with metal adsorption capacity produced by pseudoalteromonas sp. mer144 from antarctic seawater |
publishDate |
2018 |
url |
http://hdl.handle.net/11367/64489 https://doi.org/10.1007/s11356-017-0851-z https://link.springer.com/article/10.1007%2Fs11356-017-0851-z |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000425770300059 volume:25 issue:5 firstpage:4667 lastpage:4677 numberofpages:11 journal:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL http://hdl.handle.net/11367/64489 doi:10.1007/s11356-017-0851-z info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85035796043 https://link.springer.com/article/10.1007%2Fs11356-017-0851-z |
op_doi |
https://doi.org/10.1007/s11356-017-0851-z |
container_title |
Environmental Science and Pollution Research |
container_volume |
25 |
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5 |
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4667 |
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