Psychrophilic elongation factor Tu from the Antarctic Moraxella sp. Tac II 25: biochemical characterization and cloning of the encoding gene

• Main Authors: MASULLO, Mariorosario, ARCARI P., DE PAOLA B., PARMEGGIANI A., BOCCHINI V.
• Other Authors: Masullo, Mariorosario, Arcari, P., DE PAOLA, B., Parmeggiani, A., Bocchini, V.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2000
• Subjects: Antarctic; Tac; The Antarctic; Antarc*
• Online Access: http://hdl.handle.net/11367/18056

The elongation factor Tu was isolated from a psychrophilic eubacterial Antarctic Moraxella strain (MoEF-Tu) and its molecular and functional properties were determined. It catalyzed the synthesis of poly(Phe) and bound specifically guanine nucleotides with an affinity for GDP about 12-fold higher th...