Identification and functional caracterization of novel lipases/acyltransferases of yeasts
Lipases/acyltransferases have intermediate properties between lipases and acyltransferases. Although being active hydrolases, they catalyze acyltransfer reactions preferentially to hydrolysis even in an aqueous medium with a high thermodynamic activity of water in the presence of various nucleophile...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
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Online Access: | https://theses.hal.science/tel-01872513 https://theses.hal.science/tel-01872513/document https://theses.hal.science/tel-01872513/file/13-0005_Neang.pdf |
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ftunimontpellier:oai:HAL:tel-01872513v1 2023-07-16T03:53:35+02:00 Identification and functional caracterization of novel lipases/acyltransferases of yeasts Identification et caractérisation fonctionnelle de nouvelles lipases/acyltransférases de levures Neang, Pisey Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Montpellier SupAgro Eric Dubreucq Maëva Subileau 2013-04-08 https://theses.hal.science/tel-01872513 https://theses.hal.science/tel-01872513/document https://theses.hal.science/tel-01872513/file/13-0005_Neang.pdf fr fre HAL CCSD NNT: 2013NSAM0005 tel-01872513 https://theses.hal.science/tel-01872513 https://theses.hal.science/tel-01872513/document https://theses.hal.science/tel-01872513/file/13-0005_Neang.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-01872513 Sciences agricoles. Montpellier SupAgro, 2013. Français. ⟨NNT : 2013NSAM0005⟩ Candida parapsilosis Candida tropicalis Biocatalysis Biphasic aqueous medium Lipase/acyltransférase Candida viswanathii Biocatalyse Milieu biphasique aqueux [SDV.SA]Life Sciences [q-bio]/Agricultural sciences info:eu-repo/semantics/doctoralThesis Theses 2013 ftunimontpellier 2023-06-27T22:54:39Z Lipases/acyltransferases have intermediate properties between lipases and acyltransferases. Although being active hydrolases, they catalyze acyltransfer reactions preferentially to hydrolysis even in an aqueous medium with a high thermodynamic activity of water in the presence of various nucleophiles. Searching for new lipases/acyltransferases, either secreted by wild yeast strains or identified in protein sequences databases, allowed us to identify two new enzymes of this type: CvisL2 from Candida viswanathii and CtroL4a from C. tropicalis. The latter, produced by heterologous expression, has been more particularly studied and compared with the two already known, closely related, lipases/acyltransferases, CpLIP2 from C. parapsilosis and CaLIP4 from C. albicans, and with two more distantly related lipases (a new lipase AflaL0a from Aspergillus flavus and CaLA from C. antarctica, with 35 % and 31 % identity with CpLIP2, respectively). The specific catalytic behavior of the acyltransferases seems to be associated with sequence homology and phylogenetic relationships. Indeed, the three lipases/acyltransferases studied are part of a phylogenetic subgroup composed of various proteins (identity with CpLIP2 higher than 57 %), currently not characterized. Besides their acyltransfer activity, these new biocatalysts differ in properties such as their substrate selectivity, their stability in the presence of high alcohol concentration or their activity at low temperature, opening the way to new applications. Les lipases/acyltransférases présentent des propriétés intermédiaires entre les lipases et les acyltransférases. Capables de se comporter comme des hydrolases, elles catalysent cependant la réaction de transfert d'acyle préférentiellement à l'hydrolyse même en milieu aqueux à forte activité thermodynamique de l'eau en présence de divers nucléophiles. La recherche de nouvelles lipases/acyltransférases, soit sécrétées par des levures sauvages, soit identifiées parmi les séquences protéiques disponibles dans des bases ... Doctoral or Postdoctoral Thesis Antarc* Antarctica Université de Montpellier: HAL |
institution |
Open Polar |
collection |
Université de Montpellier: HAL |
op_collection_id |
ftunimontpellier |
language |
French |
topic |
Candida parapsilosis Candida tropicalis Biocatalysis Biphasic aqueous medium Lipase/acyltransférase Candida viswanathii Biocatalyse Milieu biphasique aqueux [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
spellingShingle |
Candida parapsilosis Candida tropicalis Biocatalysis Biphasic aqueous medium Lipase/acyltransférase Candida viswanathii Biocatalyse Milieu biphasique aqueux [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Neang, Pisey Identification and functional caracterization of novel lipases/acyltransferases of yeasts |
topic_facet |
Candida parapsilosis Candida tropicalis Biocatalysis Biphasic aqueous medium Lipase/acyltransférase Candida viswanathii Biocatalyse Milieu biphasique aqueux [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
description |
Lipases/acyltransferases have intermediate properties between lipases and acyltransferases. Although being active hydrolases, they catalyze acyltransfer reactions preferentially to hydrolysis even in an aqueous medium with a high thermodynamic activity of water in the presence of various nucleophiles. Searching for new lipases/acyltransferases, either secreted by wild yeast strains or identified in protein sequences databases, allowed us to identify two new enzymes of this type: CvisL2 from Candida viswanathii and CtroL4a from C. tropicalis. The latter, produced by heterologous expression, has been more particularly studied and compared with the two already known, closely related, lipases/acyltransferases, CpLIP2 from C. parapsilosis and CaLIP4 from C. albicans, and with two more distantly related lipases (a new lipase AflaL0a from Aspergillus flavus and CaLA from C. antarctica, with 35 % and 31 % identity with CpLIP2, respectively). The specific catalytic behavior of the acyltransferases seems to be associated with sequence homology and phylogenetic relationships. Indeed, the three lipases/acyltransferases studied are part of a phylogenetic subgroup composed of various proteins (identity with CpLIP2 higher than 57 %), currently not characterized. Besides their acyltransfer activity, these new biocatalysts differ in properties such as their substrate selectivity, their stability in the presence of high alcohol concentration or their activity at low temperature, opening the way to new applications. Les lipases/acyltransférases présentent des propriétés intermédiaires entre les lipases et les acyltransférases. Capables de se comporter comme des hydrolases, elles catalysent cependant la réaction de transfert d'acyle préférentiellement à l'hydrolyse même en milieu aqueux à forte activité thermodynamique de l'eau en présence de divers nucléophiles. La recherche de nouvelles lipases/acyltransférases, soit sécrétées par des levures sauvages, soit identifiées parmi les séquences protéiques disponibles dans des bases ... |
author2 |
Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Montpellier SupAgro Eric Dubreucq Maëva Subileau |
format |
Doctoral or Postdoctoral Thesis |
author |
Neang, Pisey |
author_facet |
Neang, Pisey |
author_sort |
Neang, Pisey |
title |
Identification and functional caracterization of novel lipases/acyltransferases of yeasts |
title_short |
Identification and functional caracterization of novel lipases/acyltransferases of yeasts |
title_full |
Identification and functional caracterization of novel lipases/acyltransferases of yeasts |
title_fullStr |
Identification and functional caracterization of novel lipases/acyltransferases of yeasts |
title_full_unstemmed |
Identification and functional caracterization of novel lipases/acyltransferases of yeasts |
title_sort |
identification and functional caracterization of novel lipases/acyltransferases of yeasts |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://theses.hal.science/tel-01872513 https://theses.hal.science/tel-01872513/document https://theses.hal.science/tel-01872513/file/13-0005_Neang.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
https://theses.hal.science/tel-01872513 Sciences agricoles. Montpellier SupAgro, 2013. Français. ⟨NNT : 2013NSAM0005⟩ |
op_relation |
NNT: 2013NSAM0005 tel-01872513 https://theses.hal.science/tel-01872513 https://theses.hal.science/tel-01872513/document https://theses.hal.science/tel-01872513/file/13-0005_Neang.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1771551077506744320 |