A multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields
Entomopathogenic nematodes (EPNs) are ubiquitous since they are distributed in all continents except in Antarctica. In agricultural soils, they offer a high potential for crop protection against insect pests. In France, few studies have so far described their distribution in field crops. The objecti...
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2023
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ftunimontpellier:oai:HAL:hal-04161733v1 2023-08-27T04:05:59+02:00 A multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields Depuydt, Elisabeth Ogier, Jean-Claude Cabre, Lisa Ali, Nusrat Mahieu, Patrice Villenave, Cécile Nguema-Ona, Eric Gaudriault, Sophie Diversité, Génomes & Interactions Microorganismes - Insectes Montpellier (DGIMI) Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Centre Mondial de l'Innovation Roullier Groupe Roullier Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Montpellier (UM) Chambre Régionale d'Agriculture de Midi Pyrénées Chambre Régionale d'Agriculture d'Occitanie (CRA Occitanie) Elisol Environnement INRAE 2023-06-06 https://hal.umontpellier.fr/hal-04161733 https://hal.umontpellier.fr/hal-04161733/document https://hal.umontpellier.fr/hal-04161733/file/Depuydt%20et%20al.%20-%20A%20multimethod%20approach%20improves%20the%20description%20of.pdf en eng HAL CCSD hal-04161733 https://hal.umontpellier.fr/hal-04161733 https://hal.umontpellier.fr/hal-04161733/document https://hal.umontpellier.fr/hal-04161733/file/Depuydt%20et%20al.%20-%20A%20multimethod%20approach%20improves%20the%20description%20of.pdf info:eu-repo/semantics/OpenAccess https://hal.umontpellier.fr/hal-04161733 2023 entomopathogenic nematodes biological control France multiple Galleria mellonella-baiting cycles quantitative PCR entomopathogenic nematodes biological control France multiple Galleria mellonella-baiting cycles quantitative PCR [SDE]Environmental Sciences info:eu-repo/semantics/other Proceedings 2023 ftunimontpellier 2023-08-08T22:34:10Z Entomopathogenic nematodes (EPNs) are ubiquitous since they are distributed in all continents except in Antarctica. In agricultural soils, they offer a high potential for crop protection against insect pests. In France, few studies have so far described their distribution in field crops. The objective of this study is to combine several methods to improve the detection of EPNs in cultivated soils (Figure 1). We have first used multiple Galleria mellonella-baiting cycles on soil samples collected in 43 fields cultivated in corn during early summer (2021) in Southwestern France. The emergent nematodes were identified by sequencing of the ITS region. We obtained 28% of positive plots and baited 78 isolates belonging to three species: Steinernema carpocapsae, Steinernema feltiae, Heterorhabditis bacteriophora. We showed that the multiple G. mellonellabaiting cycle method during a period of time extending to 90 days, doubles the number of positive plots and increases the number of isolates by plot. On these 43 soil samples, we also performed soil-direct nematode extraction by Oostenbrink elutriation followed by water attraction of alive nematodes. After morphometric identification of EPNs in these nematode-extracted samples, we obtained 9.3 % positive plots. We are currently carrying out quantitative PCR tests for specific detection of EPNs in these nematode-extracted samples. With the combination of those different methods, we hope to describe a more accurate inventory of EPNs present in cultivated soils. This approach may allow improvement of our knowledge on the distribution of autochtonous EPNs in cultivated soils and their potential use to control insect pests. Conference Object Antarc* Antarctica Université de Montpellier: HAL |
institution |
Open Polar |
collection |
Université de Montpellier: HAL |
op_collection_id |
ftunimontpellier |
language |
English |
topic |
entomopathogenic nematodes biological control France multiple Galleria mellonella-baiting cycles quantitative PCR entomopathogenic nematodes biological control France multiple Galleria mellonella-baiting cycles quantitative PCR [SDE]Environmental Sciences |
spellingShingle |
entomopathogenic nematodes biological control France multiple Galleria mellonella-baiting cycles quantitative PCR entomopathogenic nematodes biological control France multiple Galleria mellonella-baiting cycles quantitative PCR [SDE]Environmental Sciences Depuydt, Elisabeth Ogier, Jean-Claude Cabre, Lisa Ali, Nusrat Mahieu, Patrice Villenave, Cécile Nguema-Ona, Eric Gaudriault, Sophie A multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields |
topic_facet |
entomopathogenic nematodes biological control France multiple Galleria mellonella-baiting cycles quantitative PCR entomopathogenic nematodes biological control France multiple Galleria mellonella-baiting cycles quantitative PCR [SDE]Environmental Sciences |
description |
Entomopathogenic nematodes (EPNs) are ubiquitous since they are distributed in all continents except in Antarctica. In agricultural soils, they offer a high potential for crop protection against insect pests. In France, few studies have so far described their distribution in field crops. The objective of this study is to combine several methods to improve the detection of EPNs in cultivated soils (Figure 1). We have first used multiple Galleria mellonella-baiting cycles on soil samples collected in 43 fields cultivated in corn during early summer (2021) in Southwestern France. The emergent nematodes were identified by sequencing of the ITS region. We obtained 28% of positive plots and baited 78 isolates belonging to three species: Steinernema carpocapsae, Steinernema feltiae, Heterorhabditis bacteriophora. We showed that the multiple G. mellonellabaiting cycle method during a period of time extending to 90 days, doubles the number of positive plots and increases the number of isolates by plot. On these 43 soil samples, we also performed soil-direct nematode extraction by Oostenbrink elutriation followed by water attraction of alive nematodes. After morphometric identification of EPNs in these nematode-extracted samples, we obtained 9.3 % positive plots. We are currently carrying out quantitative PCR tests for specific detection of EPNs in these nematode-extracted samples. With the combination of those different methods, we hope to describe a more accurate inventory of EPNs present in cultivated soils. This approach may allow improvement of our knowledge on the distribution of autochtonous EPNs in cultivated soils and their potential use to control insect pests. |
author2 |
Diversité, Génomes & Interactions Microorganismes - Insectes Montpellier (DGIMI) Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Centre Mondial de l'Innovation Roullier Groupe Roullier Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Montpellier (UM) Chambre Régionale d'Agriculture de Midi Pyrénées Chambre Régionale d'Agriculture d'Occitanie (CRA Occitanie) Elisol Environnement INRAE |
format |
Conference Object |
author |
Depuydt, Elisabeth Ogier, Jean-Claude Cabre, Lisa Ali, Nusrat Mahieu, Patrice Villenave, Cécile Nguema-Ona, Eric Gaudriault, Sophie |
author_facet |
Depuydt, Elisabeth Ogier, Jean-Claude Cabre, Lisa Ali, Nusrat Mahieu, Patrice Villenave, Cécile Nguema-Ona, Eric Gaudriault, Sophie |
author_sort |
Depuydt, Elisabeth |
title |
A multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields |
title_short |
A multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields |
title_full |
A multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields |
title_fullStr |
A multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields |
title_full_unstemmed |
A multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields |
title_sort |
multimethod approach improves the description of the entomopathogenic nematode distribution in corn fields |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal.umontpellier.fr/hal-04161733 https://hal.umontpellier.fr/hal-04161733/document https://hal.umontpellier.fr/hal-04161733/file/Depuydt%20et%20al.%20-%20A%20multimethod%20approach%20improves%20the%20description%20of.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
https://hal.umontpellier.fr/hal-04161733 2023 |
op_relation |
hal-04161733 https://hal.umontpellier.fr/hal-04161733 https://hal.umontpellier.fr/hal-04161733/document https://hal.umontpellier.fr/hal-04161733/file/Depuydt%20et%20al.%20-%20A%20multimethod%20approach%20improves%20the%20description%20of.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1775346703291908096 |