A multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields
International audience Entomopathogenic nematodes (EPNs), Steinernema sp. and Heterorhabditis sp., are soil-dwelling insect parasites distributed in all continents except in Antarctica. Moreover, the detection method may bias the diagnosis of the presence of EPNs in soils. The classical method for t...
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2023
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ftunimontpellier:oai:HAL:hal-04129939v1 2023-10-01T03:51:01+02:00 A multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields Depuydt, Elisabeth Ogier, Jean-Claude Cabre, Lisa Ali, Nusrat Mahieu, Patrice Villenave, Cécile Nguema-Ona, Eric Gaudriault, Sophie Centre Mondial de l'Innovation Roullier Groupe Roullier Diversité, Génomes & Interactions Microorganismes - Insectes Montpellier (DGIMI) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Montpellier (UM) Chambre d'Agriculture des Pyrénées-Atlantiques (CA 64) Elisol Environnement International Organisation for Biological and Integrated Control Wageningen (NL), Netherlands 2023-06-06 https://hal.inrae.fr/hal-04129939 https://hal.inrae.fr/hal-04129939/document https://hal.inrae.fr/hal-04129939/file/Poster%20Elisabeth%20Depuydt.pdf en eng HAL CCSD hal-04129939 https://hal.inrae.fr/hal-04129939 https://hal.inrae.fr/hal-04129939/document https://hal.inrae.fr/hal-04129939/file/Poster%20Elisabeth%20Depuydt.pdf info:eu-repo/semantics/OpenAccess 16. Meeting of the IOBC-WRPS Working Group Biological and Integrated Control of Plant Pathogens https://hal.inrae.fr/hal-04129939 16. Meeting of the IOBC-WRPS Working Group Biological and Integrated Control of Plant Pathogens, Jun 2023, Wageningen (NL), Netherlands Entomopathogenic nematode agricultural soil biological control conservation molecular detection [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDV]Life Sciences [q-bio] info:eu-repo/semantics/conferenceObject Conference poster 2023 ftunimontpellier 2023-09-05T22:34:52Z International audience Entomopathogenic nematodes (EPNs), Steinernema sp. and Heterorhabditis sp., are soil-dwelling insect parasites distributed in all continents except in Antarctica. Moreover, the detection method may bias the diagnosis of the presence of EPNs in soils. The classical method for the detection of EPNs is the baiting method by Galleria mellonella. The objective of this study is to combine several methods to improve the detection of EPNs in cultivated soils. We assumed that a multi-approach which combines baiting with successive trapping cycles (Abd-Elgawad, 2020, Nematology) and molecular detection (Campos-Herrera et al., 2011, Annals of Applied Biology) would provide the most accurate picture of the presence and diversity of EPNs in the sampled soils. Map of the 43 sampling plots cultivated in corn in South Western France EPNs detection by successive trapping cyles (28%) Three species were isolated: S. carpocapsae, S. feltiae and H. bacteriophora. The multiplication of the baiting cycles allowed to increase the number of positive plots and the species diversity of EPNs isolated Steinernema sp. detection by ddPCR (33%) ❖ Mismatches are observed between the detection methods (e.g. molecular detection of EPNs in negative plots by baiting). ❖ Using of multi-approach improves the detection of native EPNs in soils. ❖ A good picture of the distribution of EPNs in agricultural soils is useful for their use in biological control by conservation. Conference Object Antarc* Antarctica Université de Montpellier: HAL |
institution |
Open Polar |
collection |
Université de Montpellier: HAL |
op_collection_id |
ftunimontpellier |
language |
English |
topic |
Entomopathogenic nematode agricultural soil biological control conservation molecular detection [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDV]Life Sciences [q-bio] |
spellingShingle |
Entomopathogenic nematode agricultural soil biological control conservation molecular detection [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDV]Life Sciences [q-bio] Depuydt, Elisabeth Ogier, Jean-Claude Cabre, Lisa Ali, Nusrat Mahieu, Patrice Villenave, Cécile Nguema-Ona, Eric Gaudriault, Sophie A multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields |
topic_facet |
Entomopathogenic nematode agricultural soil biological control conservation molecular detection [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDV]Life Sciences [q-bio] |
description |
International audience Entomopathogenic nematodes (EPNs), Steinernema sp. and Heterorhabditis sp., are soil-dwelling insect parasites distributed in all continents except in Antarctica. Moreover, the detection method may bias the diagnosis of the presence of EPNs in soils. The classical method for the detection of EPNs is the baiting method by Galleria mellonella. The objective of this study is to combine several methods to improve the detection of EPNs in cultivated soils. We assumed that a multi-approach which combines baiting with successive trapping cycles (Abd-Elgawad, 2020, Nematology) and molecular detection (Campos-Herrera et al., 2011, Annals of Applied Biology) would provide the most accurate picture of the presence and diversity of EPNs in the sampled soils. Map of the 43 sampling plots cultivated in corn in South Western France EPNs detection by successive trapping cyles (28%) Three species were isolated: S. carpocapsae, S. feltiae and H. bacteriophora. The multiplication of the baiting cycles allowed to increase the number of positive plots and the species diversity of EPNs isolated Steinernema sp. detection by ddPCR (33%) ❖ Mismatches are observed between the detection methods (e.g. molecular detection of EPNs in negative plots by baiting). ❖ Using of multi-approach improves the detection of native EPNs in soils. ❖ A good picture of the distribution of EPNs in agricultural soils is useful for their use in biological control by conservation. |
author2 |
Centre Mondial de l'Innovation Roullier Groupe Roullier Diversité, Génomes & Interactions Microorganismes - Insectes Montpellier (DGIMI) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Montpellier (UM) Chambre d'Agriculture des Pyrénées-Atlantiques (CA 64) Elisol Environnement International Organisation for Biological and Integrated Control |
format |
Conference Object |
author |
Depuydt, Elisabeth Ogier, Jean-Claude Cabre, Lisa Ali, Nusrat Mahieu, Patrice Villenave, Cécile Nguema-Ona, Eric Gaudriault, Sophie |
author_facet |
Depuydt, Elisabeth Ogier, Jean-Claude Cabre, Lisa Ali, Nusrat Mahieu, Patrice Villenave, Cécile Nguema-Ona, Eric Gaudriault, Sophie |
author_sort |
Depuydt, Elisabeth |
title |
A multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields |
title_short |
A multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields |
title_full |
A multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields |
title_fullStr |
A multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields |
title_full_unstemmed |
A multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields |
title_sort |
multi-approach improves the description of the entomopathogenic nematodes distribution in corn fields |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal.inrae.fr/hal-04129939 https://hal.inrae.fr/hal-04129939/document https://hal.inrae.fr/hal-04129939/file/Poster%20Elisabeth%20Depuydt.pdf |
op_coverage |
Wageningen (NL), Netherlands |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
16. Meeting of the IOBC-WRPS Working Group Biological and Integrated Control of Plant Pathogens https://hal.inrae.fr/hal-04129939 16. Meeting of the IOBC-WRPS Working Group Biological and Integrated Control of Plant Pathogens, Jun 2023, Wageningen (NL), Netherlands |
op_relation |
hal-04129939 https://hal.inrae.fr/hal-04129939 https://hal.inrae.fr/hal-04129939/document https://hal.inrae.fr/hal-04129939/file/Poster%20Elisabeth%20Depuydt.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1778530517851832320 |