Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections
International audience Tetrodotoxins (TTXs) are potentially lethal paralytic toxins that have been identified in European shellfish over recent years. Risk assessment has suggested comparatively low levels (44 µg TTX-equivalent/kg) but stresses the lack of data on occurrence. Both bacteria and dinof...
Published in: | Toxins |
---|---|
Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2021
|
Subjects: | |
Online Access: | https://hal.science/hal-03419687 https://hal.science/hal-03419687/document https://hal.science/hal-03419687/file/Reveillon-2021-Toxins-Tetrodotoxins.pdf https://doi.org/10.3390/toxins13110740 |
id |
ftunimontpellier:oai:HAL:hal-03419687v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Université de Montpellier: HAL |
op_collection_id |
ftunimontpellier |
language |
English |
topic |
emerging toxins TTXs REPHY REMI seafood contamination coastal contamination [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.IDA]Life Sciences [q-bio]/Food engineering [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain |
spellingShingle |
emerging toxins TTXs REPHY REMI seafood contamination coastal contamination [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.IDA]Life Sciences [q-bio]/Food engineering [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain Réveillon, Damien Savar, Véronique Schaefer, Estelle Chevé, Julien Halm-Lemeille, Marie-Pierre Hervio-Heath, Dominique Travers, Marie-Agnès Abadie, Eric Rolland, Jean-Luc Hess, Philipp Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
topic_facet |
emerging toxins TTXs REPHY REMI seafood contamination coastal contamination [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.IDA]Life Sciences [q-bio]/Food engineering [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain |
description |
International audience Tetrodotoxins (TTXs) are potentially lethal paralytic toxins that have been identified in European shellfish over recent years. Risk assessment has suggested comparatively low levels (44 µg TTX-equivalent/kg) but stresses the lack of data on occurrence. Both bacteria and dinoflagellates were suggested as possible biogenic sources, either from an endogenous or exogenous origin. We thus investigated TTXs in (i) 98 shellfish samples and (ii) 122 bacterial strains, isolated from French environments. We optimized a method based on mass spectrometry, using a single extraction step followed by ultrafiltration without Solid Phase Extraction and matrix-matched calibration for both shellfish and bacterial matrix. Limits of detection and quantification were 6.3 and 12.5 µg/kg for shellfish and 5.0 and 10 µg/kg for bacterial matrix, respectively. Even though bacterial matrix resulted in signal enhancement, no TTX analog was detected in any strain. Bivalves (either Crassostrea gigas or Ruditapes philippinarum) were surveyed in six French production areas over 2.5–3 month periods (2018–2019). Concentrations of TTX ranged from ‘not detected’ to a maximum of 32 µg/kg (Bay of Brest, 17 June 2019), with events lasting 2 weeks at maximum. While these results are in line with previous studies, they provide new data of TTX occurrence and confirm that the link between bacteria, bivalves and TTX is complex. |
author2 |
Unité Dynamiques des Écosystèmes Côtiers (DYNECO) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Environnement Ressources de Bretagne Nord (LERBN) Unité Littoral (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Environnement Ressources de Normandie (LERN) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) Unité Biodiversité et Environnement de la Martinique (BIODIVENV) |
format |
Article in Journal/Newspaper |
author |
Réveillon, Damien Savar, Véronique Schaefer, Estelle Chevé, Julien Halm-Lemeille, Marie-Pierre Hervio-Heath, Dominique Travers, Marie-Agnès Abadie, Eric Rolland, Jean-Luc Hess, Philipp |
author_facet |
Réveillon, Damien Savar, Véronique Schaefer, Estelle Chevé, Julien Halm-Lemeille, Marie-Pierre Hervio-Heath, Dominique Travers, Marie-Agnès Abadie, Eric Rolland, Jean-Luc Hess, Philipp |
author_sort |
Réveillon, Damien |
title |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_short |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_full |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_fullStr |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_full_unstemmed |
Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections |
title_sort |
tetrodotoxins in french bivalve mollusks—analytical methodology, environmental dynamics and screening of bacterial strain collections |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.science/hal-03419687 https://hal.science/hal-03419687/document https://hal.science/hal-03419687/file/Reveillon-2021-Toxins-Tetrodotoxins.pdf https://doi.org/10.3390/toxins13110740 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
EISSN: 2072-6651 Toxins https://hal.science/hal-03419687 Toxins, 2021, 13, pp.740. ⟨10.3390/toxins13110740⟩ https://doi.org/10.3390/ toxins13110740 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins13110740 hal-03419687 https://hal.science/hal-03419687 https://hal.science/hal-03419687/document https://hal.science/hal-03419687/file/Reveillon-2021-Toxins-Tetrodotoxins.pdf doi:10.3390/toxins13110740 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/toxins13110740 |
container_title |
Toxins |
container_volume |
13 |
container_issue |
11 |
container_start_page |
740 |
_version_ |
1797581707903238144 |
spelling |
ftunimontpellier:oai:HAL:hal-03419687v1 2024-04-28T08:16:42+00:00 Tetrodotoxins in French Bivalve Mollusks—Analytical Methodology, Environmental Dynamics and Screening of Bacterial Strain Collections Réveillon, Damien Savar, Véronique Schaefer, Estelle Chevé, Julien Halm-Lemeille, Marie-Pierre Hervio-Heath, Dominique Travers, Marie-Agnès Abadie, Eric Rolland, Jean-Luc Hess, Philipp Unité Dynamiques des Écosystèmes Côtiers (DYNECO) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Environnement Ressources de Bretagne Nord (LERBN) Unité Littoral (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Environnement Ressources de Normandie (LERN) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité Santé, Génétique et Microbiologie des Mollusques (SGMM) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) Unité Biodiversité et Environnement de la Martinique (BIODIVENV) 2021 https://hal.science/hal-03419687 https://hal.science/hal-03419687/document https://hal.science/hal-03419687/file/Reveillon-2021-Toxins-Tetrodotoxins.pdf https://doi.org/10.3390/toxins13110740 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/toxins13110740 hal-03419687 https://hal.science/hal-03419687 https://hal.science/hal-03419687/document https://hal.science/hal-03419687/file/Reveillon-2021-Toxins-Tetrodotoxins.pdf doi:10.3390/toxins13110740 info:eu-repo/semantics/OpenAccess EISSN: 2072-6651 Toxins https://hal.science/hal-03419687 Toxins, 2021, 13, pp.740. ⟨10.3390/toxins13110740⟩ https://doi.org/10.3390/ toxins13110740 emerging toxins TTXs REPHY REMI seafood contamination coastal contamination [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.IDA]Life Sciences [q-bio]/Food engineering [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain info:eu-repo/semantics/article Journal articles 2021 ftunimontpellier https://doi.org/10.3390/toxins13110740 2024-04-03T15:25:03Z International audience Tetrodotoxins (TTXs) are potentially lethal paralytic toxins that have been identified in European shellfish over recent years. Risk assessment has suggested comparatively low levels (44 µg TTX-equivalent/kg) but stresses the lack of data on occurrence. Both bacteria and dinoflagellates were suggested as possible biogenic sources, either from an endogenous or exogenous origin. We thus investigated TTXs in (i) 98 shellfish samples and (ii) 122 bacterial strains, isolated from French environments. We optimized a method based on mass spectrometry, using a single extraction step followed by ultrafiltration without Solid Phase Extraction and matrix-matched calibration for both shellfish and bacterial matrix. Limits of detection and quantification were 6.3 and 12.5 µg/kg for shellfish and 5.0 and 10 µg/kg for bacterial matrix, respectively. Even though bacterial matrix resulted in signal enhancement, no TTX analog was detected in any strain. Bivalves (either Crassostrea gigas or Ruditapes philippinarum) were surveyed in six French production areas over 2.5–3 month periods (2018–2019). Concentrations of TTX ranged from ‘not detected’ to a maximum of 32 µg/kg (Bay of Brest, 17 June 2019), with events lasting 2 weeks at maximum. While these results are in line with previous studies, they provide new data of TTX occurrence and confirm that the link between bacteria, bivalves and TTX is complex. Article in Journal/Newspaper Crassostrea gigas Université de Montpellier: HAL Toxins 13 11 740 |