The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas
International audience Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 microM Cg-Def defensin and Cg-BPI bactericidal permeability in...
Published in: | Environmental Microbiology |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2010
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Online Access: | https://hal.inrae.fr/hal-02667966 https://doi.org/10.1111/j.1462-2920.2009.02138.x |
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Université de Montpellier: HAL |
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language |
English |
topic |
OYSTER HUITRE CREUSE OmpU MESH: Adhesins Bacterial MESH: Animals MESH: Vibrio Infections MESH: Virulence MESH: Antimicrobial Cationic Peptides MESH: Blood Proteins MESH: Crassostrea MESH: Gene Deletion MESH: Genetic Complementation Test MESH: Humans MESH: Mutation MESH: Vibrio [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
OYSTER HUITRE CREUSE OmpU MESH: Adhesins Bacterial MESH: Animals MESH: Vibrio Infections MESH: Virulence MESH: Antimicrobial Cationic Peptides MESH: Blood Proteins MESH: Crassostrea MESH: Gene Deletion MESH: Genetic Complementation Test MESH: Humans MESH: Mutation MESH: Vibrio [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Duperthuy, Marylise Binesse, Johan Le Roux, Frédérique Romestand, Bernard Caro, Audrey Got, Patrice Givaudan, Alain Mazel, Didier Bachère, Evelyne Destoumieux-Garzón, Delphine The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
topic_facet |
OYSTER HUITRE CREUSE OmpU MESH: Adhesins Bacterial MESH: Animals MESH: Vibrio Infections MESH: Virulence MESH: Antimicrobial Cationic Peptides MESH: Blood Proteins MESH: Crassostrea MESH: Gene Deletion MESH: Genetic Complementation Test MESH: Humans MESH: Mutation MESH: Vibrio [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
International audience Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 microM Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. splendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan-Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction. |
author2 |
Université Montpellier 2 - Sciences et Techniques (UM2) Ecosystèmes lagunaires : organisation biologique et fonctionnement (ECOLAG) Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Plasticité du Génome Bactérien (PGB) Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Ecologie microbienne des insectes et interactions hôte-pathogène (EMIP) Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2) This work received financial support from Ifremer and CNRS. It was awarded a “chercheur(se) d'avenir” grant from the Languedoc-Roussillon region. |
format |
Article in Journal/Newspaper |
author |
Duperthuy, Marylise Binesse, Johan Le Roux, Frédérique Romestand, Bernard Caro, Audrey Got, Patrice Givaudan, Alain Mazel, Didier Bachère, Evelyne Destoumieux-Garzón, Delphine |
author_facet |
Duperthuy, Marylise Binesse, Johan Le Roux, Frédérique Romestand, Bernard Caro, Audrey Got, Patrice Givaudan, Alain Mazel, Didier Bachère, Evelyne Destoumieux-Garzón, Delphine |
author_sort |
Duperthuy, Marylise |
title |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_short |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_full |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_fullStr |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_full_unstemmed |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_sort |
major outer membrane protein ompu of vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.inrae.fr/hal-02667966 https://doi.org/10.1111/j.1462-2920.2009.02138.x |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.inrae.fr/hal-02667966 Environmental Microbiology, 2010, 12 (4), pp.951-963. ⟨10.1111/j.1462-2920.2009.02138.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1462-2920.2009.02138.x info:eu-repo/semantics/altIdentifier/pmid/20074236 hal-02667966 https://hal.inrae.fr/hal-02667966 doi:10.1111/j.1462-2920.2009.02138.x PRODINRA: 41123 PUBMED: 20074236 WOS: 000277029900010 |
op_doi |
https://doi.org/10.1111/j.1462-2920.2009.02138.x |
container_title |
Environmental Microbiology |
container_volume |
12 |
container_issue |
4 |
container_start_page |
951 |
op_container_end_page |
963 |
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1797581651991068672 |
spelling |
ftunimontpellier:oai:HAL:hal-02667966v1 2024-04-28T08:16:35+00:00 The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas Duperthuy, Marylise Binesse, Johan Le Roux, Frédérique Romestand, Bernard Caro, Audrey Got, Patrice Givaudan, Alain Mazel, Didier Bachère, Evelyne Destoumieux-Garzón, Delphine Université Montpellier 2 - Sciences et Techniques (UM2) Ecosystèmes lagunaires : organisation biologique et fonctionnement (ECOLAG) Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) Plasticité du Génome Bactérien (PGB) Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS) Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Ecologie microbienne des insectes et interactions hôte-pathogène (EMIP) Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2) This work received financial support from Ifremer and CNRS. It was awarded a “chercheur(se) d'avenir” grant from the Languedoc-Roussillon region. 2010 https://hal.inrae.fr/hal-02667966 https://doi.org/10.1111/j.1462-2920.2009.02138.x en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1462-2920.2009.02138.x info:eu-repo/semantics/altIdentifier/pmid/20074236 hal-02667966 https://hal.inrae.fr/hal-02667966 doi:10.1111/j.1462-2920.2009.02138.x PRODINRA: 41123 PUBMED: 20074236 WOS: 000277029900010 ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.inrae.fr/hal-02667966 Environmental Microbiology, 2010, 12 (4), pp.951-963. ⟨10.1111/j.1462-2920.2009.02138.x⟩ OYSTER HUITRE CREUSE OmpU MESH: Adhesins Bacterial MESH: Animals MESH: Vibrio Infections MESH: Virulence MESH: Antimicrobial Cationic Peptides MESH: Blood Proteins MESH: Crassostrea MESH: Gene Deletion MESH: Genetic Complementation Test MESH: Humans MESH: Mutation MESH: Vibrio [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2010 ftunimontpellier https://doi.org/10.1111/j.1462-2920.2009.02138.x 2024-04-10T14:34:51Z International audience Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 microM Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. splendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan-Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction. Article in Journal/Newspaper Crassostrea gigas Université de Montpellier: HAL Environmental Microbiology 12 4 951 963 |