Production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and Candida parapsilosis lipase/acyltransferase
UMR IATE AXE 4 International audience In human milk fat (HMF), palmitic acid (20-30%), the major saturated fatty acid, is mostly esterified at the sn-2 position of triacylglycerols, while unsaturated fatty acids are at the sn-1,3 positions, conversely to that occurring in vegetable oils. This study...
Published in: | Journal of Molecular Catalysis B: Enzymatic |
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Online Access: | https://hal.inrae.fr/hal-02666284 https://doi.org/10.1016/j.molcatb.2010.01.026 |
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ftunimontpellier:oai:HAL:hal-02666284v1 2024-06-23T07:47:46+00:00 Production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and Candida parapsilosis lipase/acyltransferase Tecelão, Carla Silva, Joana Dubreucq, Eric Ribeiro, Maria H. Ferreira-Dias, Suzana Instituto Superior de Agronomia, CEER, Biosystems Engineering Universidade de Lisboa = University of Lisbon = Université de Lisbonne (ULISBOA) Politécnico de Leiria Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Universidade Nova de Lisboa = NOVA University Lisbon (NOVA) 2010 https://hal.inrae.fr/hal-02666284 https://doi.org/10.1016/j.molcatb.2010.01.026 en eng HAL CCSD Elsevier [1995, vol. 1, iss. 1-2016, vol. 134] info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcatb.2010.01.026 hal-02666284 https://hal.inrae.fr/hal-02666284 doi:10.1016/j.molcatb.2010.01.026 PRODINRA: 47708 WOS: 000278926300021 ISSN: 1381-1177 EISSN: 1873-3158 Journal of Molecular Catalysis B: Enzymatic https://hal.inrae.fr/hal-02666284 Journal of Molecular Catalysis B: Enzymatic, 2010, 65 (1-4), pp.122-127. ⟨10.1016/j.molcatb.2010.01.026⟩ LIPASE HUMAN MILK FAT SUBSTITUTES OMEGA-3 POLYUNSATURATED FATTY ACIDS OPERATIONAL STABILITY STRUCTURED LIPIDS SUCCEDANE DE LAIT STABILITE OPERATIONNELLE [CHIM.CATA]Chemical Sciences/Catalysis info:eu-repo/semantics/article Journal articles 2010 ftunimontpellier https://doi.org/10.1016/j.molcatb.2010.01.026 2024-06-03T14:22:12Z UMR IATE AXE 4 International audience In human milk fat (HMF), palmitic acid (20-30%), the major saturated fatty acid, is mostly esterified at the sn-2 position of triacylglycerols, while unsaturated fatty acids are at the sn-1,3 positions, conversely to that occurring in vegetable oils. This study aims at the production of HMF substitutes by enzyme-catalyzed interesterification of tripalmitin with (i) oleic acid (system I) or (ii) omega-3 polyunsaturated fatty acids (omega-3 PUFA) (system II) in solvent-free media. Interesterification activity and batch operational stability of commercial immobilized lipases from Rhizomucor miehei (Lipozyme RM IM), Thermomyces lanuginosa (Lipozyme TL IM) and Candida antarctica (Novozym 435) from Novozymes, DK, and Candida parapsilosis lipase/acyltransferase immobilized on Accurel MP 1000 were evaluated. After 24-h reaction at 60 °C, molar incorporation of oleic acid was about 27% for all the commercial lipases tested and 9% with C. parapsilosis enzyme. Concerning omega-3 PUFA, the highest incorporations were observed with Novozym 435 (21.6%) and Lipozyme RM IM (20%), in contrast with C. parapsilosis enzyme (8.5%) and Lipozyme TL IM (8.2%). In system I, Lipozyme RM IM maintained its activity for 10 repeated 23-h batches while for Lipozyme TL IM, Novozym 435 and C. parapsilosis enzyme, linear (half-life time, t1/2 = 154 h), series-type (t1/2 = 253 h) and first-order (t1/2 = 34.5 h) deactivations were respectively observed. In system II, Lipozyme RM IM showed linear deactivation (t1/2 = 276 h), while Novozym 435 (t1/2 = 322 h) and C. parapsilosis enzyme (t1/2 = 127 h), presented series-type deactivation. Both activity and stability of the biocatalysts depended on the acyl donor used. Article in Journal/Newspaper Antarc* Antarctica Université de Montpellier: HAL Journal of Molecular Catalysis B: Enzymatic 65 1-4 122 127 |
institution |
Open Polar |
collection |
Université de Montpellier: HAL |
op_collection_id |
ftunimontpellier |
language |
English |
topic |
LIPASE HUMAN MILK FAT SUBSTITUTES OMEGA-3 POLYUNSATURATED FATTY ACIDS OPERATIONAL STABILITY STRUCTURED LIPIDS SUCCEDANE DE LAIT STABILITE OPERATIONNELLE [CHIM.CATA]Chemical Sciences/Catalysis |
spellingShingle |
LIPASE HUMAN MILK FAT SUBSTITUTES OMEGA-3 POLYUNSATURATED FATTY ACIDS OPERATIONAL STABILITY STRUCTURED LIPIDS SUCCEDANE DE LAIT STABILITE OPERATIONNELLE [CHIM.CATA]Chemical Sciences/Catalysis Tecelão, Carla Silva, Joana Dubreucq, Eric Ribeiro, Maria H. Ferreira-Dias, Suzana Production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and Candida parapsilosis lipase/acyltransferase |
topic_facet |
LIPASE HUMAN MILK FAT SUBSTITUTES OMEGA-3 POLYUNSATURATED FATTY ACIDS OPERATIONAL STABILITY STRUCTURED LIPIDS SUCCEDANE DE LAIT STABILITE OPERATIONNELLE [CHIM.CATA]Chemical Sciences/Catalysis |
description |
UMR IATE AXE 4 International audience In human milk fat (HMF), palmitic acid (20-30%), the major saturated fatty acid, is mostly esterified at the sn-2 position of triacylglycerols, while unsaturated fatty acids are at the sn-1,3 positions, conversely to that occurring in vegetable oils. This study aims at the production of HMF substitutes by enzyme-catalyzed interesterification of tripalmitin with (i) oleic acid (system I) or (ii) omega-3 polyunsaturated fatty acids (omega-3 PUFA) (system II) in solvent-free media. Interesterification activity and batch operational stability of commercial immobilized lipases from Rhizomucor miehei (Lipozyme RM IM), Thermomyces lanuginosa (Lipozyme TL IM) and Candida antarctica (Novozym 435) from Novozymes, DK, and Candida parapsilosis lipase/acyltransferase immobilized on Accurel MP 1000 were evaluated. After 24-h reaction at 60 °C, molar incorporation of oleic acid was about 27% for all the commercial lipases tested and 9% with C. parapsilosis enzyme. Concerning omega-3 PUFA, the highest incorporations were observed with Novozym 435 (21.6%) and Lipozyme RM IM (20%), in contrast with C. parapsilosis enzyme (8.5%) and Lipozyme TL IM (8.2%). In system I, Lipozyme RM IM maintained its activity for 10 repeated 23-h batches while for Lipozyme TL IM, Novozym 435 and C. parapsilosis enzyme, linear (half-life time, t1/2 = 154 h), series-type (t1/2 = 253 h) and first-order (t1/2 = 34.5 h) deactivations were respectively observed. In system II, Lipozyme RM IM showed linear deactivation (t1/2 = 276 h), while Novozym 435 (t1/2 = 322 h) and C. parapsilosis enzyme (t1/2 = 127 h), presented series-type deactivation. Both activity and stability of the biocatalysts depended on the acyl donor used. |
author2 |
Instituto Superior de Agronomia, CEER, Biosystems Engineering Universidade de Lisboa = University of Lisbon = Université de Lisbonne (ULISBOA) Politécnico de Leiria Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Universidade Nova de Lisboa = NOVA University Lisbon (NOVA) |
format |
Article in Journal/Newspaper |
author |
Tecelão, Carla Silva, Joana Dubreucq, Eric Ribeiro, Maria H. Ferreira-Dias, Suzana |
author_facet |
Tecelão, Carla Silva, Joana Dubreucq, Eric Ribeiro, Maria H. Ferreira-Dias, Suzana |
author_sort |
Tecelão, Carla |
title |
Production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and Candida parapsilosis lipase/acyltransferase |
title_short |
Production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and Candida parapsilosis lipase/acyltransferase |
title_full |
Production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and Candida parapsilosis lipase/acyltransferase |
title_fullStr |
Production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and Candida parapsilosis lipase/acyltransferase |
title_full_unstemmed |
Production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and Candida parapsilosis lipase/acyltransferase |
title_sort |
production of human milk fat substitutes enriched in omega-3 polyunsaturated fatty acids using immobilized commercial lipases and candida parapsilosis lipase/acyltransferase |
publisher |
HAL CCSD |
publishDate |
2010 |
url |
https://hal.inrae.fr/hal-02666284 https://doi.org/10.1016/j.molcatb.2010.01.026 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ISSN: 1381-1177 EISSN: 1873-3158 Journal of Molecular Catalysis B: Enzymatic https://hal.inrae.fr/hal-02666284 Journal of Molecular Catalysis B: Enzymatic, 2010, 65 (1-4), pp.122-127. ⟨10.1016/j.molcatb.2010.01.026⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcatb.2010.01.026 hal-02666284 https://hal.inrae.fr/hal-02666284 doi:10.1016/j.molcatb.2010.01.026 PRODINRA: 47708 WOS: 000278926300021 |
op_doi |
https://doi.org/10.1016/j.molcatb.2010.01.026 |
container_title |
Journal of Molecular Catalysis B: Enzymatic |
container_volume |
65 |
container_issue |
1-4 |
container_start_page |
122 |
op_container_end_page |
127 |
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1802637912826707968 |