Evaluation of deep eutectic solvents as new media for Candida Antarctica B lipase catalyzed reactions
This study aimed at analyzing the advantages and limitations of several deep eutectic solvents (DESs) as ‘green solvents’ for biotransformation using immobilized Candida antarctica lipase B as catalyst. The transesterification of vinyl laurate was chosen as model reaction and the influence of substr...
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Online Access: | https://hal.science/hal-01267982 https://doi.org/10.1016/j.procbio.2012.07.027 |
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ftunimontpellier:oai:HAL:hal-01267982v1 2023-07-16T03:54:36+02:00 Evaluation of deep eutectic solvents as new media for Candida Antarctica B lipase catalyzed reactions Durand, Erwann Lecomte, Jérôme Barea, Bruno Piombo, Georges Dubreucq, Eric Villeneuve, Pierre Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) 2012 https://hal.science/hal-01267982 https://doi.org/10.1016/j.procbio.2012.07.027 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2012.07.027 hal-01267982 https://hal.science/hal-01267982 doi:10.1016/j.procbio.2012.07.027 PRODINRA: 188170 WOS: 000313851700049 ISSN: 1359-5113 Process Biochemistry https://hal.science/hal-01267982 Process Biochemistry, 2012, 47 (12), pp.2081-2089. ⟨10.1016/j.procbio.2012.07.027⟩ deep eutectic solven lipase Candida antarctica biocatalysis transesterification [SDV.SA]Life Sciences [q-bio]/Agricultural sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology info:eu-repo/semantics/article Journal articles 2012 ftunimontpellier https://doi.org/10.1016/j.procbio.2012.07.027 2023-06-27T22:57:09Z This study aimed at analyzing the advantages and limitations of several deep eutectic solvents (DESs) as ‘green solvents’ for biotransformation using immobilized Candida antarctica lipase B as catalyst. The transesterification of vinyl laurate was chosen as model reaction and the influence of substrate polarity was assessed using alcohols of various chain lengths. Results showed that grinding of immobilized lipase was essential parameters for good lipase activity. Moreover, in our model reaction some hydrogen-bond donor component from the DES can compete with the alcoholysis reaction. Indeed, side reactions were observed with DES based on dicarboxylic acid or ethylene glycol, leading to some limitations of their use. However, the results showed that other DESs such as choline chloride:urea and choline chloride:glycerol could exhibit high activity and selectivity making them promising solvents for lipase-catalyzed reactions. Finally, the best DES’s specific activity – and stability up to five days incubation time – were analyzed and compared with conventional organic solvents. Experiments revealed that iCALB is less influenced by the chain length of alcohol in DES than organic solvents and it is preserves its activity with minimally destructive to protein structure. Article in Journal/Newspaper Antarc* Antarctica Université de Montpellier: HAL Process Biochemistry 47 12 2081 2089 |
institution |
Open Polar |
collection |
Université de Montpellier: HAL |
op_collection_id |
ftunimontpellier |
language |
English |
topic |
deep eutectic solven lipase Candida antarctica biocatalysis transesterification [SDV.SA]Life Sciences [q-bio]/Agricultural sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
spellingShingle |
deep eutectic solven lipase Candida antarctica biocatalysis transesterification [SDV.SA]Life Sciences [q-bio]/Agricultural sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Durand, Erwann Lecomte, Jérôme Barea, Bruno Piombo, Georges Dubreucq, Eric Villeneuve, Pierre Evaluation of deep eutectic solvents as new media for Candida Antarctica B lipase catalyzed reactions |
topic_facet |
deep eutectic solven lipase Candida antarctica biocatalysis transesterification [SDV.SA]Life Sciences [q-bio]/Agricultural sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
description |
This study aimed at analyzing the advantages and limitations of several deep eutectic solvents (DESs) as ‘green solvents’ for biotransformation using immobilized Candida antarctica lipase B as catalyst. The transesterification of vinyl laurate was chosen as model reaction and the influence of substrate polarity was assessed using alcohols of various chain lengths. Results showed that grinding of immobilized lipase was essential parameters for good lipase activity. Moreover, in our model reaction some hydrogen-bond donor component from the DES can compete with the alcoholysis reaction. Indeed, side reactions were observed with DES based on dicarboxylic acid or ethylene glycol, leading to some limitations of their use. However, the results showed that other DESs such as choline chloride:urea and choline chloride:glycerol could exhibit high activity and selectivity making them promising solvents for lipase-catalyzed reactions. Finally, the best DES’s specific activity – and stability up to five days incubation time – were analyzed and compared with conventional organic solvents. Experiments revealed that iCALB is less influenced by the chain length of alcohol in DES than organic solvents and it is preserves its activity with minimally destructive to protein structure. |
author2 |
Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
format |
Article in Journal/Newspaper |
author |
Durand, Erwann Lecomte, Jérôme Barea, Bruno Piombo, Georges Dubreucq, Eric Villeneuve, Pierre |
author_facet |
Durand, Erwann Lecomte, Jérôme Barea, Bruno Piombo, Georges Dubreucq, Eric Villeneuve, Pierre |
author_sort |
Durand, Erwann |
title |
Evaluation of deep eutectic solvents as new media for Candida Antarctica B lipase catalyzed reactions |
title_short |
Evaluation of deep eutectic solvents as new media for Candida Antarctica B lipase catalyzed reactions |
title_full |
Evaluation of deep eutectic solvents as new media for Candida Antarctica B lipase catalyzed reactions |
title_fullStr |
Evaluation of deep eutectic solvents as new media for Candida Antarctica B lipase catalyzed reactions |
title_full_unstemmed |
Evaluation of deep eutectic solvents as new media for Candida Antarctica B lipase catalyzed reactions |
title_sort |
evaluation of deep eutectic solvents as new media for candida antarctica b lipase catalyzed reactions |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.science/hal-01267982 https://doi.org/10.1016/j.procbio.2012.07.027 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ISSN: 1359-5113 Process Biochemistry https://hal.science/hal-01267982 Process Biochemistry, 2012, 47 (12), pp.2081-2089. ⟨10.1016/j.procbio.2012.07.027⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2012.07.027 hal-01267982 https://hal.science/hal-01267982 doi:10.1016/j.procbio.2012.07.027 PRODINRA: 188170 WOS: 000313851700049 |
op_doi |
https://doi.org/10.1016/j.procbio.2012.07.027 |
container_title |
Process Biochemistry |
container_volume |
47 |
container_issue |
12 |
container_start_page |
2081 |
op_container_end_page |
2089 |
_version_ |
1771540865853947904 |