Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures
International audience Since 2008, the emergent virus OsHV-1µvar has provoked massive mortality events in Crassostrea gigas spat and juveniles in France. Since 2012, mortality driven by the pathogenic bacteria Vibrio aestuarianus has stricken market-sized adults. A hypothesis to explain the sudden i...
Published in: | Veterinary Research |
---|---|
Main Authors: | , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2015
|
Subjects: | |
Online Access: | https://hal.science/hal-01259809 https://hal.science/hal-01259809/document https://hal.science/hal-01259809/file/Azema-2015-VetRes-Can.pdf https://doi.org/10.1186/s13567-015-0282-0 |
id |
ftunimontpellier:oai:HAL:hal-01259809v1 |
---|---|
record_format |
openpolar |
spelling |
ftunimontpellier:oai:HAL:hal-01259809v1 2024-04-28T08:16:33+00:00 Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures Azéma, Patrick Travers, Marie-Agnès de Lorgeril, Julien Tourbiez, Delphine Dégremont, Lionel Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) 2015 https://hal.science/hal-01259809 https://hal.science/hal-01259809/document https://hal.science/hal-01259809/file/Azema-2015-VetRes-Can.pdf https://doi.org/10.1186/s13567-015-0282-0 en eng HAL CCSD BioMed Central info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-015-0282-0 hal-01259809 https://hal.science/hal-01259809 https://hal.science/hal-01259809/document https://hal.science/hal-01259809/file/Azema-2015-VetRes-Can.pdf doi:10.1186/s13567-015-0282-0 info:eu-repo/semantics/OpenAccess ISSN: 0928-4249 EISSN: 1297-9716 Veterinary Research https://hal.science/hal-01259809 Veterinary Research, 2015, 46 (1), pp.139. ⟨10.1186/s13567-015-0282-0⟩ http://www.veterinaryresearch.org/content/46/1/139 [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] info:eu-repo/semantics/article Journal articles 2015 ftunimontpellier https://doi.org/10.1186/s13567-015-0282-0 2024-04-10T14:40:21Z International audience Since 2008, the emergent virus OsHV-1µvar has provoked massive mortality events in Crassostrea gigas spat and juveniles in France. Since 2012, mortality driven by the pathogenic bacteria Vibrio aestuarianus has stricken market-sized adults. A hypothesis to explain the sudden increase in mortality observed in France since 2012 is that selective pressure due to recurrent viral infections could have led to a higher susceptibility of adults to Vibrio infection. In our study, two OsHV-1-resistant lines (AS and BS) and their respective controls (AC and BC) were experimentally challenged in the laboratory to determine their level of susceptibility to V. aestuarianus infection. At the juvenile stage, the selected lines exhibited lower mortality (14 and 33%) than the control lines (71 and 80%), suggesting dual-resistance to OsHV-1 and V. aestuarianus in C. gigas. Interestingly, this pattern was not observed at the adult stage, where higher mortality was detected for AS (68%) and BC (62%) than AC (39%) and BS (49%). These results were confirmed by the analysis of the expression of 31 immune-related genes in unchallenged oysters. Differential gene expression discriminated oysters according to their susceptibility to infection at both the juvenile and adult stages, suggesting that resistance to V. aestuarianus infection resulted in complex interactions between the genotype, stage of development and immunity status. Finally, survivors of the V. aestuarianus challenge at the juvenile stage still exhibited significant mortality at the adult stage during a second and third V. aestuarianus challenge, indicating that these survivors were not genetically resistant. Article in Journal/Newspaper Crassostrea gigas Université de Montpellier: HAL Veterinary Research 46 1 |
institution |
Open Polar |
collection |
Université de Montpellier: HAL |
op_collection_id |
ftunimontpellier |
language |
English |
topic |
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] |
spellingShingle |
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] Azéma, Patrick Travers, Marie-Agnès de Lorgeril, Julien Tourbiez, Delphine Dégremont, Lionel Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures |
topic_facet |
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] |
description |
International audience Since 2008, the emergent virus OsHV-1µvar has provoked massive mortality events in Crassostrea gigas spat and juveniles in France. Since 2012, mortality driven by the pathogenic bacteria Vibrio aestuarianus has stricken market-sized adults. A hypothesis to explain the sudden increase in mortality observed in France since 2012 is that selective pressure due to recurrent viral infections could have led to a higher susceptibility of adults to Vibrio infection. In our study, two OsHV-1-resistant lines (AS and BS) and their respective controls (AC and BC) were experimentally challenged in the laboratory to determine their level of susceptibility to V. aestuarianus infection. At the juvenile stage, the selected lines exhibited lower mortality (14 and 33%) than the control lines (71 and 80%), suggesting dual-resistance to OsHV-1 and V. aestuarianus in C. gigas. Interestingly, this pattern was not observed at the adult stage, where higher mortality was detected for AS (68%) and BC (62%) than AC (39%) and BS (49%). These results were confirmed by the analysis of the expression of 31 immune-related genes in unchallenged oysters. Differential gene expression discriminated oysters according to their susceptibility to infection at both the juvenile and adult stages, suggesting that resistance to V. aestuarianus infection resulted in complex interactions between the genotype, stage of development and immunity status. Finally, survivors of the V. aestuarianus challenge at the juvenile stage still exhibited significant mortality at the adult stage during a second and third V. aestuarianus challenge, indicating that these survivors were not genetically resistant. |
author2 |
Unité Amélioration génétique, Santé animale et Environnement (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) |
format |
Article in Journal/Newspaper |
author |
Azéma, Patrick Travers, Marie-Agnès de Lorgeril, Julien Tourbiez, Delphine Dégremont, Lionel |
author_facet |
Azéma, Patrick Travers, Marie-Agnès de Lorgeril, Julien Tourbiez, Delphine Dégremont, Lionel |
author_sort |
Azéma, Patrick |
title |
Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures |
title_short |
Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures |
title_full |
Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures |
title_fullStr |
Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures |
title_full_unstemmed |
Can selection for resistance to OsHV-1 infection modify susceptibility to Vibrio aestuarianus infection in Crassostrea gigas? First insights from experimental challenges using primary and successive exposures |
title_sort |
can selection for resistance to oshv-1 infection modify susceptibility to vibrio aestuarianus infection in crassostrea gigas? first insights from experimental challenges using primary and successive exposures |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-01259809 https://hal.science/hal-01259809/document https://hal.science/hal-01259809/file/Azema-2015-VetRes-Can.pdf https://doi.org/10.1186/s13567-015-0282-0 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0928-4249 EISSN: 1297-9716 Veterinary Research https://hal.science/hal-01259809 Veterinary Research, 2015, 46 (1), pp.139. ⟨10.1186/s13567-015-0282-0⟩ http://www.veterinaryresearch.org/content/46/1/139 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-015-0282-0 hal-01259809 https://hal.science/hal-01259809 https://hal.science/hal-01259809/document https://hal.science/hal-01259809/file/Azema-2015-VetRes-Can.pdf doi:10.1186/s13567-015-0282-0 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s13567-015-0282-0 |
container_title |
Veterinary Research |
container_volume |
46 |
container_issue |
1 |
_version_ |
1797581629618651136 |