Antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus
Bioprospecting in unusual marine environments provides an innovative approach to search novel biomolecules with antibiofilm activity. Antarctic sponge-associated bacteria belonging to Colwellia, Pseudoalteromonas, Shewanella and Winogradskyella genera were evaluated for their ability to contrast the...
Published in: | Journal of Marine Science and Engineering |
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Online Access: | http://hdl.handle.net/11570/3207487 https://doi.org/10.3390/jmse9030243 |
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ftunimessinairis:oai:iris.unime.it:11570/3207487 2024-02-11T09:58:29+01:00 Antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus Rizzo C. Zammuto V. Lo Giudice A. Rizzo M. G. Spano A. Lagana' P. Martinez M. Guglielmino S. Gugliandolo C. Rizzo, C. Zammuto, V. Lo Giudice, A. Rizzo, M. G. Spano, A. Lagana', P. Martinez, M. Guglielmino, S. Gugliandolo, C. 2021 STAMPA http://hdl.handle.net/11570/3207487 https://doi.org/10.3390/jmse9030243 eng eng MDPI AG info:eu-repo/semantics/altIdentifier/wos/WOS:000633761800001 volume:9 issue:3 firstpage:1 lastpage:16 numberofpages:16 journal:JOURNAL OF MARINE SCIENCE AND ENGINEERING http://hdl.handle.net/11570/3207487 doi:10.3390/jmse9030243 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85102172749 Antarctic bacteria Biofilm Sponge-associated bacteria info:eu-repo/semantics/article 2021 ftunimessinairis https://doi.org/10.3390/jmse9030243 2024-01-24T17:45:21Z Bioprospecting in unusual marine environments provides an innovative approach to search novel biomolecules with antibiofilm activity. Antarctic sponge-associated bacteria belonging to Colwellia, Pseudoalteromonas, Shewanella and Winogradskyella genera were evaluated for their ability to contrast the biofilm formation by Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213, as model organisms. All strains were able to produce biofilm at both 4 and 25 °C, with the highest production being for Colwellia, Shewanella and Winogradskyella strains at 4 °C after 24 h. Antibiofilm activity of cell-free supernatants (CFSs) differed among strains and on the basis of their incubation temperature (CFSs4°C and CFSs25°C). The major activity was observed by CFSs4°C against S. aureus and CFSs25°C against P. aeruginosa, without demonstrating a bactericidal effect on their growth. Furthermore, the antibiofilm activity of crude extracts from Colwellia sp. GW185, Shewanella sp. CAL606, and Winogradskyella sp. CAL396 was also evaluated and visualized by confocal laser scanning microscopic images. Results based on the surface-coating assay and surface tension measurements suggest that CFSs and the crude extracts may act as biosurfactants inhibiting the first adhesion of P. aeruginosa and S. aureus. The CFSs and the novel biopolymers may be useful in applicative perspectives for pharmaceutical and environmental purposes. Article in Journal/Newspaper Antarc* Antarctic Università degli Studi di Messina: IRIS Antarctic Journal of Marine Science and Engineering 9 3 243 |
institution |
Open Polar |
collection |
Università degli Studi di Messina: IRIS |
op_collection_id |
ftunimessinairis |
language |
English |
topic |
Antarctic bacteria Biofilm Sponge-associated bacteria |
spellingShingle |
Antarctic bacteria Biofilm Sponge-associated bacteria Rizzo C. Zammuto V. Lo Giudice A. Rizzo M. G. Spano A. Lagana' P. Martinez M. Guglielmino S. Gugliandolo C. Antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus |
topic_facet |
Antarctic bacteria Biofilm Sponge-associated bacteria |
description |
Bioprospecting in unusual marine environments provides an innovative approach to search novel biomolecules with antibiofilm activity. Antarctic sponge-associated bacteria belonging to Colwellia, Pseudoalteromonas, Shewanella and Winogradskyella genera were evaluated for their ability to contrast the biofilm formation by Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213, as model organisms. All strains were able to produce biofilm at both 4 and 25 °C, with the highest production being for Colwellia, Shewanella and Winogradskyella strains at 4 °C after 24 h. Antibiofilm activity of cell-free supernatants (CFSs) differed among strains and on the basis of their incubation temperature (CFSs4°C and CFSs25°C). The major activity was observed by CFSs4°C against S. aureus and CFSs25°C against P. aeruginosa, without demonstrating a bactericidal effect on their growth. Furthermore, the antibiofilm activity of crude extracts from Colwellia sp. GW185, Shewanella sp. CAL606, and Winogradskyella sp. CAL396 was also evaluated and visualized by confocal laser scanning microscopic images. Results based on the surface-coating assay and surface tension measurements suggest that CFSs and the crude extracts may act as biosurfactants inhibiting the first adhesion of P. aeruginosa and S. aureus. The CFSs and the novel biopolymers may be useful in applicative perspectives for pharmaceutical and environmental purposes. |
author2 |
Rizzo, C. Zammuto, V. Lo Giudice, A. Rizzo, M. G. Spano, A. Lagana', P. Martinez, M. Guglielmino, S. Gugliandolo, C. |
format |
Article in Journal/Newspaper |
author |
Rizzo C. Zammuto V. Lo Giudice A. Rizzo M. G. Spano A. Lagana' P. Martinez M. Guglielmino S. Gugliandolo C. |
author_facet |
Rizzo C. Zammuto V. Lo Giudice A. Rizzo M. G. Spano A. Lagana' P. Martinez M. Guglielmino S. Gugliandolo C. |
author_sort |
Rizzo C. |
title |
Antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus |
title_short |
Antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus |
title_full |
Antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus |
title_fullStr |
Antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus |
title_full_unstemmed |
Antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus |
title_sort |
antibiofilm activity of antarctic sponge-associated bacteria against pseudomonas aeruginosa and staphylococcus aureus |
publisher |
MDPI AG |
publishDate |
2021 |
url |
http://hdl.handle.net/11570/3207487 https://doi.org/10.3390/jmse9030243 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000633761800001 volume:9 issue:3 firstpage:1 lastpage:16 numberofpages:16 journal:JOURNAL OF MARINE SCIENCE AND ENGINEERING http://hdl.handle.net/11570/3207487 doi:10.3390/jmse9030243 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85102172749 |
op_doi |
https://doi.org/10.3390/jmse9030243 |
container_title |
Journal of Marine Science and Engineering |
container_volume |
9 |
container_issue |
3 |
container_start_page |
243 |
_version_ |
1790594142590468096 |