EFFECTS ON DEVELOPMENT, GROWTH-HORMONE, AND THYROID-HORMONE SYSTEMS IN EYED EGGS AND YOLK-SAC LARVAE OF ATLANTIC SALMON (Salmo salar) CONTINUOUSLY EXPOSED TO 3,3,4,4-TETRACHLOROBIPHENYL (PCB-77)
Thyroid hormones (triiodothyronine, T3; and thyroxine, T4) play significant roles in development, metamorphosis, metabolism, homeostasis, cellular proliferation, and differentiation, for which the effects are mediated through thyroid hormone receptors (TRα and TRβ). Similarly, the insulin-like growt...
Published in: | Journal of Toxicology and Environmental Health, Part A |
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Online Access: | http://hdl.handle.net/11570/2693368 https://doi.org/10.1080/15287394.2014.887422 |
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ftunimessinairis:oai:iris.unime.it:11570/2693368 2024-02-11T10:02:10+01:00 EFFECTS ON DEVELOPMENT, GROWTH-HORMONE, AND THYROID-HORMONE SYSTEMS IN EYED EGGS AND YOLK-SAC LARVAE OF ATLANTIC SALMON (Salmo salar) CONTINUOUSLY EXPOSED TO 3,3,4,4-TETRACHLOROBIPHENYL (PCB-77) Augustine, Arukwe Marianne, Olufsen Marianne, D. Hansen CICERO, Nicola Augustine, Arukwe Marianne, Olufsen Nicola, Cicero Marianne, D. Hansen 2014 STAMPA http://hdl.handle.net/11570/2693368 https://doi.org/10.1080/15287394.2014.887422 eng eng info:eu-repo/semantics/altIdentifier/pmid/24754393 info:eu-repo/semantics/altIdentifier/wos/000335118500009 volume:77 firstpage:1 lastpage:574 numberofpages:586 journal:JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH. PART A http://hdl.handle.net/11570/2693368 doi:10.1080/15287394.2014.887422 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84899729538 info:eu-repo/semantics/article 2014 ftunimessinairis https://doi.org/10.1080/15287394.2014.887422 2024-01-24T17:40:04Z Thyroid hormones (triiodothyronine, T3; and thyroxine, T4) play significant roles in development, metamorphosis, metabolism, homeostasis, cellular proliferation, and differentiation, for which the effects are mediated through thyroid hormone receptors (TRα and TRβ). Similarly, the insulin-like growth factor (IGF) is involved in growth and development through regulation of somatic growth. This study was designed to examine the effects of the dioxin- like 3,3’,4,4’-tetrachlorobiphenyl (PCB-77) on responses related to growth and thyroid hormone system in eyed eggs and yolk-sac larvae of Atlantic salmon. Salmon eggs were continuously exposed to two waterborne concentrations of PCB-77 (1 or 10 ng/L) over a period of 50 d covering hatching and through yolk-sac absorption stages. Sampling was performed regularly throughout the exposure period and at different time intervals. Gene expression patterns were performed on whole-body homogenate at age 500, 548, 632, 674, and 716 dd (dd: day degrees) using quantitative polymerase chain reaction (PCR). Total T3 (TT3) and total T4 (TT4) were measured using radioimmunoassay (RIA). Data showed that 10 ng PCB-77 increased dioiodinase 2 (Dio2) at 500 dd and both PCB-77 concentrations decreased dio2 expression. PCB-77 elevated cellular TT3 at 500 dd and was lowered at 548 dd only at 10 ng. Otherwise, time-related reduction was not affected by PCB-77 exposure as observed for the rest of the exposure period. For TT4, 1 ng PCB-77 produced a rise at 500 dd, and an apparent concentration decrease at 548 dd, before a total inhibition at 632 dd. The IGF-1 and IGF-1R were variably affected by PCB-77. For IGF-2, PCB-77 produced a concentration- dependent increase at 548 dd, and thereafter an elevation (1 ng) and fall (10 ng) at 632 dd. TRβ mRNA demonstrated PCB-77 related increases during the exposure period, and this effect returned to control levels at 716 dd. For TRα, a rise was noted only after exposure to 10 ng PCB-77 at 500 dd. Overall, the present study demonstrates some possible ... Article in Journal/Newspaper Atlantic salmon Salmo salar Università degli Studi di Messina: IRIS Journal of Toxicology and Environmental Health, Part A 77 9-11 574 586 |
institution |
Open Polar |
collection |
Università degli Studi di Messina: IRIS |
op_collection_id |
ftunimessinairis |
language |
English |
description |
Thyroid hormones (triiodothyronine, T3; and thyroxine, T4) play significant roles in development, metamorphosis, metabolism, homeostasis, cellular proliferation, and differentiation, for which the effects are mediated through thyroid hormone receptors (TRα and TRβ). Similarly, the insulin-like growth factor (IGF) is involved in growth and development through regulation of somatic growth. This study was designed to examine the effects of the dioxin- like 3,3’,4,4’-tetrachlorobiphenyl (PCB-77) on responses related to growth and thyroid hormone system in eyed eggs and yolk-sac larvae of Atlantic salmon. Salmon eggs were continuously exposed to two waterborne concentrations of PCB-77 (1 or 10 ng/L) over a period of 50 d covering hatching and through yolk-sac absorption stages. Sampling was performed regularly throughout the exposure period and at different time intervals. Gene expression patterns were performed on whole-body homogenate at age 500, 548, 632, 674, and 716 dd (dd: day degrees) using quantitative polymerase chain reaction (PCR). Total T3 (TT3) and total T4 (TT4) were measured using radioimmunoassay (RIA). Data showed that 10 ng PCB-77 increased dioiodinase 2 (Dio2) at 500 dd and both PCB-77 concentrations decreased dio2 expression. PCB-77 elevated cellular TT3 at 500 dd and was lowered at 548 dd only at 10 ng. Otherwise, time-related reduction was not affected by PCB-77 exposure as observed for the rest of the exposure period. For TT4, 1 ng PCB-77 produced a rise at 500 dd, and an apparent concentration decrease at 548 dd, before a total inhibition at 632 dd. The IGF-1 and IGF-1R were variably affected by PCB-77. For IGF-2, PCB-77 produced a concentration- dependent increase at 548 dd, and thereafter an elevation (1 ng) and fall (10 ng) at 632 dd. TRβ mRNA demonstrated PCB-77 related increases during the exposure period, and this effect returned to control levels at 716 dd. For TRα, a rise was noted only after exposure to 10 ng PCB-77 at 500 dd. Overall, the present study demonstrates some possible ... |
author2 |
Augustine, Arukwe Marianne, Olufsen Nicola, Cicero Marianne, D. Hansen |
format |
Article in Journal/Newspaper |
author |
Augustine, Arukwe Marianne, Olufsen Marianne, D. Hansen CICERO, Nicola |
spellingShingle |
Augustine, Arukwe Marianne, Olufsen Marianne, D. Hansen CICERO, Nicola EFFECTS ON DEVELOPMENT, GROWTH-HORMONE, AND THYROID-HORMONE SYSTEMS IN EYED EGGS AND YOLK-SAC LARVAE OF ATLANTIC SALMON (Salmo salar) CONTINUOUSLY EXPOSED TO 3,3,4,4-TETRACHLOROBIPHENYL (PCB-77) |
author_facet |
Augustine, Arukwe Marianne, Olufsen Marianne, D. Hansen CICERO, Nicola |
author_sort |
Augustine, Arukwe |
title |
EFFECTS ON DEVELOPMENT, GROWTH-HORMONE, AND THYROID-HORMONE SYSTEMS IN EYED EGGS AND YOLK-SAC LARVAE OF ATLANTIC SALMON (Salmo salar) CONTINUOUSLY EXPOSED TO 3,3,4,4-TETRACHLOROBIPHENYL (PCB-77) |
title_short |
EFFECTS ON DEVELOPMENT, GROWTH-HORMONE, AND THYROID-HORMONE SYSTEMS IN EYED EGGS AND YOLK-SAC LARVAE OF ATLANTIC SALMON (Salmo salar) CONTINUOUSLY EXPOSED TO 3,3,4,4-TETRACHLOROBIPHENYL (PCB-77) |
title_full |
EFFECTS ON DEVELOPMENT, GROWTH-HORMONE, AND THYROID-HORMONE SYSTEMS IN EYED EGGS AND YOLK-SAC LARVAE OF ATLANTIC SALMON (Salmo salar) CONTINUOUSLY EXPOSED TO 3,3,4,4-TETRACHLOROBIPHENYL (PCB-77) |
title_fullStr |
EFFECTS ON DEVELOPMENT, GROWTH-HORMONE, AND THYROID-HORMONE SYSTEMS IN EYED EGGS AND YOLK-SAC LARVAE OF ATLANTIC SALMON (Salmo salar) CONTINUOUSLY EXPOSED TO 3,3,4,4-TETRACHLOROBIPHENYL (PCB-77) |
title_full_unstemmed |
EFFECTS ON DEVELOPMENT, GROWTH-HORMONE, AND THYROID-HORMONE SYSTEMS IN EYED EGGS AND YOLK-SAC LARVAE OF ATLANTIC SALMON (Salmo salar) CONTINUOUSLY EXPOSED TO 3,3,4,4-TETRACHLOROBIPHENYL (PCB-77) |
title_sort |
effects on development, growth-hormone, and thyroid-hormone systems in eyed eggs and yolk-sac larvae of atlantic salmon (salmo salar) continuously exposed to 3,3,4,4-tetrachlorobiphenyl (pcb-77) |
publishDate |
2014 |
url |
http://hdl.handle.net/11570/2693368 https://doi.org/10.1080/15287394.2014.887422 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/24754393 info:eu-repo/semantics/altIdentifier/wos/000335118500009 volume:77 firstpage:1 lastpage:574 numberofpages:586 journal:JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH. PART A http://hdl.handle.net/11570/2693368 doi:10.1080/15287394.2014.887422 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84899729538 |
op_doi |
https://doi.org/10.1080/15287394.2014.887422 |
container_title |
Journal of Toxicology and Environmental Health, Part A |
container_volume |
77 |
container_issue |
9-11 |
container_start_page |
574 |
op_container_end_page |
586 |
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1790598076313894912 |