Is routine DNA barcoding an efficient tool to reveal introductions of alien macroalgae? A case study of Agardhiella subulata (Solieriaceae, Rhodophyta) in Cape Peloro lagoon (Sicily, Italy)
A comprehensive assessment of macroalgal biodiversity was conducted along the coasts of the Straits of Messina (Italy) using DNA barcoding. This approach confirmed the introduction of Agardhiella subulata in Cape Peloro lagoon (Messina, Italy), an aquatic system in which shellfish importation and st...
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ftunimessinairis:oai:iris.unime.it:11570/1906008 2024-04-21T08:00:44+00:00 Is routine DNA barcoding an efficient tool to reveal introductions of alien macroalgae? A case study of Agardhiella subulata (Solieriaceae, Rhodophyta) in Cape Peloro lagoon (Sicily, Italy) MANGHISI, ANTONIO MORABITO, Marina BERTUCCIO, CLARA GENOVESE, Giuseppa Line LE GALL Arnaud COULOUX Corinne CRUAUD Manghisi, Antonio Morabito, Marina Bertuccio, Clara Line LE, Gall Arnaud, Couloux Corinne, Cruaud Genovese, Giuseppa 2010 STAMPA http://hdl.handle.net/11570/1906008 http://connect.barcodeoflife.net/page/cryptogamie-vol-31-no-4-2010 http://www.cryptogamie.com/pagint_en/recherche/affich_art.php?cid=459 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000286153800005 volume:31 issue:4 firstpage:423 lastpage:433 numberofpages:11 journal:CRYPTOGAMIE. ALGOLOGIE http://hdl.handle.net/11570/1906008 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-78951489925 http://connect.barcodeoflife.net/page/cryptogamie-vol-31-no-4-2010 http://www.cryptogamie.com/pagint_en/recherche/affich_art.php?cid=459 Agardhiella subulata DNA barcoding alien specie Mediterranean Rhodophyta Strait of Messina transitional environment COI-5’ region rbcL gene info:eu-repo/semantics/article 2010 ftunimessinairis 2024-03-25T18:15:05Z A comprehensive assessment of macroalgal biodiversity was conducted along the coasts of the Straits of Messina (Italy) using DNA barcoding. This approach confirmed the introduction of Agardhiella subulata in Cape Peloro lagoon (Messina, Italy), an aquatic system in which shellfish importation and stabulation are widely practiced. Based on a comparison of rbcL sequences, a sample from lake Ganzirri was shown to be conspecific with A. subulata from North Carolina (USA). Suspecting that shells of the oyster Crassostrea gigas constitute the main vector for introduction of exogenous species, individuals from imported stocks were screened for the presence of epizoic algae, which were then sequenced for the DNA barcode region (5’ end of COI gene). A small specimen growing on an oyster shell was assigned with confidence to A. subulata based on its COI sequence, a task that would have been virtually impossible based on morphological characters of the immature specimen. Based on the example here, we conclude that monitoring algal diversity using DNA-barcoding would clearly be a rapid and efficient approach to highlight the introduction of alien species. Article in Journal/Newspaper Crassostrea gigas Università degli Studi di Messina: IRIS |
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Università degli Studi di Messina: IRIS |
op_collection_id |
ftunimessinairis |
language |
English |
topic |
Agardhiella subulata DNA barcoding alien specie Mediterranean Rhodophyta Strait of Messina transitional environment COI-5’ region rbcL gene |
spellingShingle |
Agardhiella subulata DNA barcoding alien specie Mediterranean Rhodophyta Strait of Messina transitional environment COI-5’ region rbcL gene MANGHISI, ANTONIO MORABITO, Marina BERTUCCIO, CLARA GENOVESE, Giuseppa Line LE GALL Arnaud COULOUX Corinne CRUAUD Is routine DNA barcoding an efficient tool to reveal introductions of alien macroalgae? A case study of Agardhiella subulata (Solieriaceae, Rhodophyta) in Cape Peloro lagoon (Sicily, Italy) |
topic_facet |
Agardhiella subulata DNA barcoding alien specie Mediterranean Rhodophyta Strait of Messina transitional environment COI-5’ region rbcL gene |
description |
A comprehensive assessment of macroalgal biodiversity was conducted along the coasts of the Straits of Messina (Italy) using DNA barcoding. This approach confirmed the introduction of Agardhiella subulata in Cape Peloro lagoon (Messina, Italy), an aquatic system in which shellfish importation and stabulation are widely practiced. Based on a comparison of rbcL sequences, a sample from lake Ganzirri was shown to be conspecific with A. subulata from North Carolina (USA). Suspecting that shells of the oyster Crassostrea gigas constitute the main vector for introduction of exogenous species, individuals from imported stocks were screened for the presence of epizoic algae, which were then sequenced for the DNA barcode region (5’ end of COI gene). A small specimen growing on an oyster shell was assigned with confidence to A. subulata based on its COI sequence, a task that would have been virtually impossible based on morphological characters of the immature specimen. Based on the example here, we conclude that monitoring algal diversity using DNA-barcoding would clearly be a rapid and efficient approach to highlight the introduction of alien species. |
author2 |
Manghisi, Antonio Morabito, Marina Bertuccio, Clara Line LE, Gall Arnaud, Couloux Corinne, Cruaud Genovese, Giuseppa |
format |
Article in Journal/Newspaper |
author |
MANGHISI, ANTONIO MORABITO, Marina BERTUCCIO, CLARA GENOVESE, Giuseppa Line LE GALL Arnaud COULOUX Corinne CRUAUD |
author_facet |
MANGHISI, ANTONIO MORABITO, Marina BERTUCCIO, CLARA GENOVESE, Giuseppa Line LE GALL Arnaud COULOUX Corinne CRUAUD |
author_sort |
MANGHISI, ANTONIO |
title |
Is routine DNA barcoding an efficient tool to reveal introductions of alien macroalgae? A case study of Agardhiella subulata (Solieriaceae, Rhodophyta) in Cape Peloro lagoon (Sicily, Italy) |
title_short |
Is routine DNA barcoding an efficient tool to reveal introductions of alien macroalgae? A case study of Agardhiella subulata (Solieriaceae, Rhodophyta) in Cape Peloro lagoon (Sicily, Italy) |
title_full |
Is routine DNA barcoding an efficient tool to reveal introductions of alien macroalgae? A case study of Agardhiella subulata (Solieriaceae, Rhodophyta) in Cape Peloro lagoon (Sicily, Italy) |
title_fullStr |
Is routine DNA barcoding an efficient tool to reveal introductions of alien macroalgae? A case study of Agardhiella subulata (Solieriaceae, Rhodophyta) in Cape Peloro lagoon (Sicily, Italy) |
title_full_unstemmed |
Is routine DNA barcoding an efficient tool to reveal introductions of alien macroalgae? A case study of Agardhiella subulata (Solieriaceae, Rhodophyta) in Cape Peloro lagoon (Sicily, Italy) |
title_sort |
is routine dna barcoding an efficient tool to reveal introductions of alien macroalgae? a case study of agardhiella subulata (solieriaceae, rhodophyta) in cape peloro lagoon (sicily, italy) |
publishDate |
2010 |
url |
http://hdl.handle.net/11570/1906008 http://connect.barcodeoflife.net/page/cryptogamie-vol-31-no-4-2010 http://www.cryptogamie.com/pagint_en/recherche/affich_art.php?cid=459 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000286153800005 volume:31 issue:4 firstpage:423 lastpage:433 numberofpages:11 journal:CRYPTOGAMIE. ALGOLOGIE http://hdl.handle.net/11570/1906008 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-78951489925 http://connect.barcodeoflife.net/page/cryptogamie-vol-31-no-4-2010 http://www.cryptogamie.com/pagint_en/recherche/affich_art.php?cid=459 |
_version_ |
1796941163073110016 |