Conserved transactivating and pro-apoptotic functions of hepadnaviral X protein in ortho- and avihepadnaviruses.
Two established activities of the multifunctional human hepatitis B virus X-protein are its transactivating and pro-apoptotic potential. We analysed whether X-proteins from other orthohepadnaviruses and the newly discovered avihepadnaviral X-proteins have similar functions as HBx. Previously, we hav...
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Online Access: | http://hdl.handle.net/11570/1896240 https://doi.org/10.1038/sj.onc.1205916 |
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ftunimessinairis:oai:iris.unime.it:11570/1896240 2024-04-14T08:08:00+00:00 Conserved transactivating and pro-apoptotic functions of hepadnaviral X protein in ortho- and avihepadnaviruses. R. Schuster E. Hildt S. Chang O. Terradillos R. Lanford W. H. Gerlich H. Will S. Schaefer POLLICINO, Teresa R., Schuster E., Hildt S., Chang O., Terradillo Pollicino, Teresa R., Lanford W. H., Gerlich H., Will S., Schaefer 2002 ELETTRONICO http://hdl.handle.net/11570/1896240 https://doi.org/10.1038/sj.onc.1205916 eng eng info:eu-repo/semantics/altIdentifier/pmid/12242658 info:eu-repo/semantics/altIdentifier/wos/WOS:000178202300006 volume:21 issue:43 firstpage:6606 lastpage:6613 numberofpages:7 journal:ONCOGENE http://hdl.handle.net/11570/1896240 doi:10.1038/sj.onc.1205916 info:eu-repo/semantics/altIdentifier/scopus/eid=2-s2.0-0037179902 http://dx.doi.org/10.1038/sj.onc.1205916 HBx transcriptional transactivation Apoptosi HCC Avihepadnavirus DHBV PROGRAMMED CELL-DEATH HEPATITIS-B VIRUS info:eu-repo/semantics/article 2002 ftunimessinairis https://doi.org/10.1038/sj.onc.1205916 2024-03-25T18:14:23Z Two established activities of the multifunctional human hepatitis B virus X-protein are its transactivating and pro-apoptotic potential. We analysed whether X-proteins from other orthohepadnaviruses and the newly discovered avihepadnaviral X-proteins have similar functions as HBx. Previously, we have shown that HBx suppresses oncogenic transformation of primary rat embryo fibroblasts (REF) by induction of apoptosis. Using this system, we found that the wildtype X-proteins of woodchuck, ground squirrel, arctic squirrel and woolly monkey hepatitis B virus exhibit similar levels of pro-apoptotic activity as HBx, whereas mutants with carboxyterminal deletions were severely impaired in this activity. A strong correlation between the pro-apoptotic and transactivating abilities of the mammalian X-proteins was found. The newly discovered avihepadnaviral X-like proteins showed similar and Raf-MAPK pathway-dependent transactivating abilities and induced apoptosis in the REF-assay. Our data indicate that the transactivating and pro-apoptotic activities reside in the carboxyterminal half of orthohepadnaviral X and are conserved in avihepadnaviral X-proteins. Article in Journal/Newspaper Arctic Università degli Studi di Messina: IRIS Arctic Oncogene 21 43 6606 6613 |
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Università degli Studi di Messina: IRIS |
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ftunimessinairis |
language |
English |
topic |
HBx transcriptional transactivation Apoptosi HCC Avihepadnavirus DHBV PROGRAMMED CELL-DEATH HEPATITIS-B VIRUS |
spellingShingle |
HBx transcriptional transactivation Apoptosi HCC Avihepadnavirus DHBV PROGRAMMED CELL-DEATH HEPATITIS-B VIRUS R. Schuster E. Hildt S. Chang O. Terradillos R. Lanford W. H. Gerlich H. Will S. Schaefer POLLICINO, Teresa Conserved transactivating and pro-apoptotic functions of hepadnaviral X protein in ortho- and avihepadnaviruses. |
topic_facet |
HBx transcriptional transactivation Apoptosi HCC Avihepadnavirus DHBV PROGRAMMED CELL-DEATH HEPATITIS-B VIRUS |
description |
Two established activities of the multifunctional human hepatitis B virus X-protein are its transactivating and pro-apoptotic potential. We analysed whether X-proteins from other orthohepadnaviruses and the newly discovered avihepadnaviral X-proteins have similar functions as HBx. Previously, we have shown that HBx suppresses oncogenic transformation of primary rat embryo fibroblasts (REF) by induction of apoptosis. Using this system, we found that the wildtype X-proteins of woodchuck, ground squirrel, arctic squirrel and woolly monkey hepatitis B virus exhibit similar levels of pro-apoptotic activity as HBx, whereas mutants with carboxyterminal deletions were severely impaired in this activity. A strong correlation between the pro-apoptotic and transactivating abilities of the mammalian X-proteins was found. The newly discovered avihepadnaviral X-like proteins showed similar and Raf-MAPK pathway-dependent transactivating abilities and induced apoptosis in the REF-assay. Our data indicate that the transactivating and pro-apoptotic activities reside in the carboxyterminal half of orthohepadnaviral X and are conserved in avihepadnaviral X-proteins. |
author2 |
R., Schuster E., Hildt S., Chang O., Terradillo Pollicino, Teresa R., Lanford W. H., Gerlich H., Will S., Schaefer |
format |
Article in Journal/Newspaper |
author |
R. Schuster E. Hildt S. Chang O. Terradillos R. Lanford W. H. Gerlich H. Will S. Schaefer POLLICINO, Teresa |
author_facet |
R. Schuster E. Hildt S. Chang O. Terradillos R. Lanford W. H. Gerlich H. Will S. Schaefer POLLICINO, Teresa |
author_sort |
R. Schuster |
title |
Conserved transactivating and pro-apoptotic functions of hepadnaviral X protein in ortho- and avihepadnaviruses. |
title_short |
Conserved transactivating and pro-apoptotic functions of hepadnaviral X protein in ortho- and avihepadnaviruses. |
title_full |
Conserved transactivating and pro-apoptotic functions of hepadnaviral X protein in ortho- and avihepadnaviruses. |
title_fullStr |
Conserved transactivating and pro-apoptotic functions of hepadnaviral X protein in ortho- and avihepadnaviruses. |
title_full_unstemmed |
Conserved transactivating and pro-apoptotic functions of hepadnaviral X protein in ortho- and avihepadnaviruses. |
title_sort |
conserved transactivating and pro-apoptotic functions of hepadnaviral x protein in ortho- and avihepadnaviruses. |
publishDate |
2002 |
url |
http://hdl.handle.net/11570/1896240 https://doi.org/10.1038/sj.onc.1205916 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/12242658 info:eu-repo/semantics/altIdentifier/wos/WOS:000178202300006 volume:21 issue:43 firstpage:6606 lastpage:6613 numberofpages:7 journal:ONCOGENE http://hdl.handle.net/11570/1896240 doi:10.1038/sj.onc.1205916 info:eu-repo/semantics/altIdentifier/scopus/eid=2-s2.0-0037179902 http://dx.doi.org/10.1038/sj.onc.1205916 |
op_doi |
https://doi.org/10.1038/sj.onc.1205916 |
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Oncogene |
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21 |
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43 |
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6606 |
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6613 |
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