Protective effects of prostaglandins in the isolated gastric mucosa of eel, Anguilla anguilla
The protective effect of endogenous prostaglandins on the fish gastric mucosa was evaluated by studying the effect of indomethacin and aspirin, known cyclooxigenase inhibitors, on the mucosal ulceration in the isolated gastric sacs of Anguilla anguilla. Gastric sacs devoid of muscle layers were incu...
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ftunimessinairis:oai:iris.unime.it:11570/1889338 2024-04-21T07:45:34+00:00 Protective effects of prostaglandins in the isolated gastric mucosa of eel, Anguilla anguilla TRISCHITTA, Francesca Ross DENARO, Maria Gabr. FAGGIO, Caterina MANDOLFINO, Mattia Grazia Trischitta, Francesca Ro Denaro, Maria Gabr. Faggio, Caterina Mandolfino, Mattia Grazia 1998 http://hdl.handle.net/11570/1889338 http://ISI PT: J, DT: Meeting Abstract unknown info:eu-repo/semantics/altIdentifier/pmid/11083517 info:eu-repo/semantics/altIdentifier/wos/WOS:000072071800132 volume:435 issue:4 firstpage:109 lastpage:109 numberofpages:1 journal:PFLÜGERS ARCHIV http://hdl.handle.net/11570/1889338 info:eu-repo/semantics/altIdentifier/scopus/WOS:000072071800132 http://ISI PT: J, DT: Meeting Abstract Acid secretion Alkaline secretion Cytoprotection Fish stomach Prostaglandins info:eu-repo/semantics/article 1998 ftunimessinairis 2024-03-25T18:13:51Z The protective effect of endogenous prostaglandins on the fish gastric mucosa was evaluated by studying the effect of indomethacin and aspirin, known cyclooxigenase inhibitors, on the mucosal ulceration in the isolated gastric sacs of Anguilla anguilla. Gastric sacs devoid of muscle layers were incubated in the presence of indomethacin (10-4 mol · 1-1) or aspirin (10-4 mol · 1-1) in different experimental conditions. Both the anti-inflammatory drugs produced ulcers, but the effects were more severe in the presence of histamine and in the absence of HCO3/- in the incubation bath. The effects of prostaglandin E2 (PGE2) on acid secretion rate (J(H)) and on alkaline secretion rate (J(OH)) were evaluated (with the aid of the pH stat method) in isolated gastric mucosa mounted in Ussing chambers. We found that PGE2 (10-8-10-5 mol · 1-1) increased J(H) in a dose-dependent manner. In tissues pretreated with luminal omeprazole (10-4 mol · 1-1), PGE2 stimulated gastric alkaline secretion. It was nullified by serosal removal of HCO3/- or Na+ and by serosal ouabain (10-4 mol · 1-1). These results suggested that prostaglandins also exert their protective effects in fish gastric mucosa. This protection seems partially due to a stimulation of exogenous HCO3/- transport from the serosal to the mucosal side. It is likely that this transport is an active transcellular mechanism coupled to Na+ transport. Article in Journal/Newspaper Anguilla anguilla Università degli Studi di Messina: IRIS |
institution |
Open Polar |
collection |
Università degli Studi di Messina: IRIS |
op_collection_id |
ftunimessinairis |
language |
unknown |
topic |
Acid secretion Alkaline secretion Cytoprotection Fish stomach Prostaglandins |
spellingShingle |
Acid secretion Alkaline secretion Cytoprotection Fish stomach Prostaglandins TRISCHITTA, Francesca Ross DENARO, Maria Gabr. FAGGIO, Caterina MANDOLFINO, Mattia Grazia Protective effects of prostaglandins in the isolated gastric mucosa of eel, Anguilla anguilla |
topic_facet |
Acid secretion Alkaline secretion Cytoprotection Fish stomach Prostaglandins |
description |
The protective effect of endogenous prostaglandins on the fish gastric mucosa was evaluated by studying the effect of indomethacin and aspirin, known cyclooxigenase inhibitors, on the mucosal ulceration in the isolated gastric sacs of Anguilla anguilla. Gastric sacs devoid of muscle layers were incubated in the presence of indomethacin (10-4 mol · 1-1) or aspirin (10-4 mol · 1-1) in different experimental conditions. Both the anti-inflammatory drugs produced ulcers, but the effects were more severe in the presence of histamine and in the absence of HCO3/- in the incubation bath. The effects of prostaglandin E2 (PGE2) on acid secretion rate (J(H)) and on alkaline secretion rate (J(OH)) were evaluated (with the aid of the pH stat method) in isolated gastric mucosa mounted in Ussing chambers. We found that PGE2 (10-8-10-5 mol · 1-1) increased J(H) in a dose-dependent manner. In tissues pretreated with luminal omeprazole (10-4 mol · 1-1), PGE2 stimulated gastric alkaline secretion. It was nullified by serosal removal of HCO3/- or Na+ and by serosal ouabain (10-4 mol · 1-1). These results suggested that prostaglandins also exert their protective effects in fish gastric mucosa. This protection seems partially due to a stimulation of exogenous HCO3/- transport from the serosal to the mucosal side. It is likely that this transport is an active transcellular mechanism coupled to Na+ transport. |
author2 |
Trischitta, Francesca Ro Denaro, Maria Gabr. Faggio, Caterina Mandolfino, Mattia Grazia |
format |
Article in Journal/Newspaper |
author |
TRISCHITTA, Francesca Ross DENARO, Maria Gabr. FAGGIO, Caterina MANDOLFINO, Mattia Grazia |
author_facet |
TRISCHITTA, Francesca Ross DENARO, Maria Gabr. FAGGIO, Caterina MANDOLFINO, Mattia Grazia |
author_sort |
TRISCHITTA, Francesca Ross |
title |
Protective effects of prostaglandins in the isolated gastric mucosa of eel, Anguilla anguilla |
title_short |
Protective effects of prostaglandins in the isolated gastric mucosa of eel, Anguilla anguilla |
title_full |
Protective effects of prostaglandins in the isolated gastric mucosa of eel, Anguilla anguilla |
title_fullStr |
Protective effects of prostaglandins in the isolated gastric mucosa of eel, Anguilla anguilla |
title_full_unstemmed |
Protective effects of prostaglandins in the isolated gastric mucosa of eel, Anguilla anguilla |
title_sort |
protective effects of prostaglandins in the isolated gastric mucosa of eel, anguilla anguilla |
publishDate |
1998 |
url |
http://hdl.handle.net/11570/1889338 http://ISI PT: J, DT: Meeting Abstract |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/11083517 info:eu-repo/semantics/altIdentifier/wos/WOS:000072071800132 volume:435 issue:4 firstpage:109 lastpage:109 numberofpages:1 journal:PFLÜGERS ARCHIV http://hdl.handle.net/11570/1889338 info:eu-repo/semantics/altIdentifier/scopus/WOS:000072071800132 http://ISI PT: J, DT: Meeting Abstract |
_version_ |
1796940670196252672 |