Design, production and purification of a novel recombinant gonadotropin-releasing hormone associated peptide as a spawning inducing agent for fish

• Published in: Protein Expression and Purification
• Main Authors: Mohammadzadeh, Sedigheh, Moradian, Fatemeh, Yeganeh, Sakineh, Falahatkar, Bahram, Milla, Sylvain
• Other Authors: Sari Agricultural Sciences and Natural Resources University, University of Guilan, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: Decapeptide; GAP; Half-life; Proteolytic Stability; rGnRH; [SDV.BIO]Life Sciences [q-bio]/Biotechnology; [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]; [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction; [SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology; [SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny; Talon; Beluga; Beluga*
• Online Access: https://hal.science/hal-02884030; https://hal.science/hal-02884030/document; https://hal.science/hal-02884030/file/Preprint%20Mohammadzadeh%20S%20et%20al.%202020.pdf; https://doi.org/10.1016/j.pep.2019.105510

International audience GnRH is a neuropeptide known to regulate reproduction in vertebrates. The purpose of this study was to design and produce recombinant gonadotropin-releasing hormone associated peptide (rGnRH/GAP) as an alternative of the previous GnRHs and native extracted hormone from tissue,...