Plasmid-like replication of a minimal streptococcal integrative and conjugative element
International audience Integrative and conjugative elements (ICEs) are mobile genetic elements encoding their own excision from a replicon of their bacterial host, transfer by conjugation to a recipient bacterium and reintegration for maintenance. The conjugation, recombination and regulation module...
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ftunilorrainehal:oai:HAL:hal-01476043v1 2023-07-30T04:04:10+02:00 Plasmid-like replication of a minimal streptococcal integrative and conjugative element Carraro, Nicolas Libante, Virginie Charron-Bourgoin, Florence Guédon, Gérard Morel, Catherine Leblond, Pierre Dynamique des Génomes et Adaptation Microbienne (DynAMic) Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) 2016-04-01 https://hal.univ-lorraine.fr/hal-01476043 https://doi.org/10.1099/mic.0.000219 en eng HAL CCSD Microbiology Society info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000219 info:eu-repo/semantics/altIdentifier/pmid/26825653 hal-01476043 https://hal.univ-lorraine.fr/hal-01476043 doi:10.1099/mic.0.000219 PRODINRA: 346177 PUBMED: 26825653 WOS: 000377847000003 ISSN: 1350-0872 EISSN: 1465-2080 Microbiology https://hal.univ-lorraine.fr/hal-01476043 Microbiology, 2016, 162 (4), pp.622 - 632. ⟨10.1099/mic.0.000219⟩ http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000219#tab2 Mobilizable genomic islands Rolling circle replication Horizontal gene-transfer IV secretion Gram-positive bacteria Lactis subsp lactis Cis-mobilization Escherichia-coli Antibiotic-resistance Thermophilus [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2016 ftunilorrainehal https://doi.org/10.1099/mic.0.00021910.1099/mic.0.000219#tab2 2023-07-17T01:31:13Z International audience Integrative and conjugative elements (ICEs) are mobile genetic elements encoding their own excision from a replicon of their bacterial host, transfer by conjugation to a recipient bacterium and reintegration for maintenance. The conjugation, recombination and regulation modules of ICEs of the ICESt3 family are grouped together in a region called the ICE 'core region'. In addition to this core region, elements belonging to this family carry a highly variable region including cargo genes that could be involved in bacterial adaptation or in the maintenance of the element. Although ICEs are a major class of mobile elements through bacterial genomes, the functionality of an element encoding only its excision, transfer, integration and regulation has never been demonstrated experimentally. We engineered MiniICESt3, an artificial ICE derived from ICESt3, devoid of its cargo genes and thus only harbouring the core region. The functionality of this minimal element was assessed. MiniICESt3 was found to be able to excise at a rate of 3.1 %, transfer with a frequency of 1.0 x 10(-5) transconjugants per donor cell and stably maintain by site-specific integration into the 3' end of the fda gene, the same as ICESt3. Furthermore, MiniICESt3 was found in similar to 10 copies per chromosome, this multicopy state likely contributing to its stability for >100 generations even in the absence of selection. Therefore, although ICEs were primarily assumed to only replicate along with the chromosome, our results uncovered extrachromosomal rolling-circle replicating plasmid-like forms of MiniICESt3. Article in Journal/Newspaper ice core Université de Lorraine: HAL Microbiology 162 4 622 632 |
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Open Polar |
collection |
Université de Lorraine: HAL |
op_collection_id |
ftunilorrainehal |
language |
English |
topic |
Mobilizable genomic islands Rolling circle replication Horizontal gene-transfer IV secretion Gram-positive bacteria Lactis subsp lactis Cis-mobilization Escherichia-coli Antibiotic-resistance Thermophilus [SDV]Life Sciences [q-bio] |
spellingShingle |
Mobilizable genomic islands Rolling circle replication Horizontal gene-transfer IV secretion Gram-positive bacteria Lactis subsp lactis Cis-mobilization Escherichia-coli Antibiotic-resistance Thermophilus [SDV]Life Sciences [q-bio] Carraro, Nicolas Libante, Virginie Charron-Bourgoin, Florence Guédon, Gérard Morel, Catherine Leblond, Pierre Plasmid-like replication of a minimal streptococcal integrative and conjugative element |
topic_facet |
Mobilizable genomic islands Rolling circle replication Horizontal gene-transfer IV secretion Gram-positive bacteria Lactis subsp lactis Cis-mobilization Escherichia-coli Antibiotic-resistance Thermophilus [SDV]Life Sciences [q-bio] |
description |
International audience Integrative and conjugative elements (ICEs) are mobile genetic elements encoding their own excision from a replicon of their bacterial host, transfer by conjugation to a recipient bacterium and reintegration for maintenance. The conjugation, recombination and regulation modules of ICEs of the ICESt3 family are grouped together in a region called the ICE 'core region'. In addition to this core region, elements belonging to this family carry a highly variable region including cargo genes that could be involved in bacterial adaptation or in the maintenance of the element. Although ICEs are a major class of mobile elements through bacterial genomes, the functionality of an element encoding only its excision, transfer, integration and regulation has never been demonstrated experimentally. We engineered MiniICESt3, an artificial ICE derived from ICESt3, devoid of its cargo genes and thus only harbouring the core region. The functionality of this minimal element was assessed. MiniICESt3 was found to be able to excise at a rate of 3.1 %, transfer with a frequency of 1.0 x 10(-5) transconjugants per donor cell and stably maintain by site-specific integration into the 3' end of the fda gene, the same as ICESt3. Furthermore, MiniICESt3 was found in similar to 10 copies per chromosome, this multicopy state likely contributing to its stability for >100 generations even in the absence of selection. Therefore, although ICEs were primarily assumed to only replicate along with the chromosome, our results uncovered extrachromosomal rolling-circle replicating plasmid-like forms of MiniICESt3. |
author2 |
Dynamique des Génomes et Adaptation Microbienne (DynAMic) Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) |
format |
Article in Journal/Newspaper |
author |
Carraro, Nicolas Libante, Virginie Charron-Bourgoin, Florence Guédon, Gérard Morel, Catherine Leblond, Pierre |
author_facet |
Carraro, Nicolas Libante, Virginie Charron-Bourgoin, Florence Guédon, Gérard Morel, Catherine Leblond, Pierre |
author_sort |
Carraro, Nicolas |
title |
Plasmid-like replication of a minimal streptococcal integrative and conjugative element |
title_short |
Plasmid-like replication of a minimal streptococcal integrative and conjugative element |
title_full |
Plasmid-like replication of a minimal streptococcal integrative and conjugative element |
title_fullStr |
Plasmid-like replication of a minimal streptococcal integrative and conjugative element |
title_full_unstemmed |
Plasmid-like replication of a minimal streptococcal integrative and conjugative element |
title_sort |
plasmid-like replication of a minimal streptococcal integrative and conjugative element |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.univ-lorraine.fr/hal-01476043 https://doi.org/10.1099/mic.0.000219 |
genre |
ice core |
genre_facet |
ice core |
op_source |
ISSN: 1350-0872 EISSN: 1465-2080 Microbiology https://hal.univ-lorraine.fr/hal-01476043 Microbiology, 2016, 162 (4), pp.622 - 632. ⟨10.1099/mic.0.000219⟩ http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000219#tab2 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1099/mic.0.000219 info:eu-repo/semantics/altIdentifier/pmid/26825653 hal-01476043 https://hal.univ-lorraine.fr/hal-01476043 doi:10.1099/mic.0.000219 PRODINRA: 346177 PUBMED: 26825653 WOS: 000377847000003 |
op_doi |
https://doi.org/10.1099/mic.0.00021910.1099/mic.0.000219#tab2 |
container_title |
Microbiology |
container_volume |
162 |
container_issue |
4 |
container_start_page |
622 |
op_container_end_page |
632 |
_version_ |
1772815376325804032 |