Effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore
Ethanol storage and lipid extraction can alter the isotopic composition of animal tissues, which can bias dietary estimates calculated by stable isotope mixing models (SIMMs). We examined the effects of ethanol storage and lipid extraction on [delta]13C, [delta]15N, and [delta]34S values measured in...
Published in: | Journal of Mammalogy |
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2019
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Online Access: | https://repozitorij.uni-lj.si/IzpisGradiva.php?id=106175 https://plus.si.cobiss.net/opac7/bib/5301926?lang=sl |
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ftuniljubljanair:oai:repozitorij.uni-lj.si:IzpisGradiva.php-id-106175 2023-05-15T18:41:57+02:00 Effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore Javornik, Jernej Hopkins, John III Zavadlav, Saša Levanič, Tom Lojen, Sonja Polak, Tomaž Jerina, Klemen 2019-02-06 https://repozitorij.uni-lj.si/IzpisGradiva.php?id=106175 https://plus.si.cobiss.net/opac7/bib/5301926?lang=sl eng eng info:eu-repo/semantics/altIdentifier/doi/10.1093/jmammal/gyy187 info:eu-repo/grantAgreement/ARRS/Raziskovalni%20projekti%20-%20temeljni/J4-7362 https://repozitorij.uni-lj.si/IzpisGradiva.php?id=106175 https://plus.si.cobiss.net/opac7/bib/5301926?lang=sl info:eu-repo/semantics/embargoedAccess lipid extraction sample preservation sulfur stable isotopes Ursus arctos ekstrakcija lipidov shranjevanje vzorcev stabilni izotopi žvepla varstvo narave rjavi medved upravljanje z divjadjo zveri info:eu-repo/classification/udc/630*15(045)=111 info:eu-repo/semantics/other info:eu-repo/semantics/publishedVersion 2019 ftuniljubljanair https://doi.org/10.1093/jmammal/gyy187 2021-12-06T10:05:09Z Ethanol storage and lipid extraction can alter the isotopic composition of animal tissues, which can bias dietary estimates calculated by stable isotope mixing models (SIMMs). We examined the effects of ethanol storage and lipid extraction on [delta]13C, [delta]15N, and [delta]34S values measured in brown bear (Ursus arctos) muscles and livers. We also used isotopic data from our experiment to understand the effect of ethanol storage and lipid extraction on dietary contributions calculated by SIMMs. We found that ethanol storage and lipid extraction caused small increases in [delta]13C values for both muscles (ethanol storage: +0.4 - 0.5 prom., lipid extraction: +0.4 - 0.4 prom.) and liver (ethanol storage: +0.6 - 0.3prom., lipid extraction: +0.8 - 0.5 prom.). In contrast, [delta]15N and [delta]34S values did not change when stored in ethanol or when lipids were extracted from tissues. Ethanol storage and lipid extraction had negligible effects on estimated dietary contributions. We show that a relatively high lipid content in the muscles and livers of some large-bodied terrestrial omnivores do not necessarily have an effect on dietary estimates that rely on carbon stable isotopes. Our results suggest that ethanol storage could be a valuable alternative method for preserving animal tissue prior to stable isotope analysis when freezing or drying is impractical. Nevertheless, further research is needed on the mechanisms that control changes in stable isotope composition in tissues stored in ethanol. We recommend investigating the effects of ethanol on stable isotope values in species and tissues of interest before storing samples in ethanol. Other/Unknown Material Ursus arctos Repository of the University of Ljubljana (RUL) Journal of Mammalogy 100 1 150 157 |
institution |
Open Polar |
collection |
Repository of the University of Ljubljana (RUL) |
op_collection_id |
ftuniljubljanair |
language |
English |
topic |
lipid extraction sample preservation sulfur stable isotopes Ursus arctos ekstrakcija lipidov shranjevanje vzorcev stabilni izotopi žvepla varstvo narave rjavi medved upravljanje z divjadjo zveri info:eu-repo/classification/udc/630*15(045)=111 |
spellingShingle |
lipid extraction sample preservation sulfur stable isotopes Ursus arctos ekstrakcija lipidov shranjevanje vzorcev stabilni izotopi žvepla varstvo narave rjavi medved upravljanje z divjadjo zveri info:eu-repo/classification/udc/630*15(045)=111 Javornik, Jernej Hopkins, John III Zavadlav, Saša Levanič, Tom Lojen, Sonja Polak, Tomaž Jerina, Klemen Effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore |
topic_facet |
lipid extraction sample preservation sulfur stable isotopes Ursus arctos ekstrakcija lipidov shranjevanje vzorcev stabilni izotopi žvepla varstvo narave rjavi medved upravljanje z divjadjo zveri info:eu-repo/classification/udc/630*15(045)=111 |
description |
Ethanol storage and lipid extraction can alter the isotopic composition of animal tissues, which can bias dietary estimates calculated by stable isotope mixing models (SIMMs). We examined the effects of ethanol storage and lipid extraction on [delta]13C, [delta]15N, and [delta]34S values measured in brown bear (Ursus arctos) muscles and livers. We also used isotopic data from our experiment to understand the effect of ethanol storage and lipid extraction on dietary contributions calculated by SIMMs. We found that ethanol storage and lipid extraction caused small increases in [delta]13C values for both muscles (ethanol storage: +0.4 - 0.5 prom., lipid extraction: +0.4 - 0.4 prom.) and liver (ethanol storage: +0.6 - 0.3prom., lipid extraction: +0.8 - 0.5 prom.). In contrast, [delta]15N and [delta]34S values did not change when stored in ethanol or when lipids were extracted from tissues. Ethanol storage and lipid extraction had negligible effects on estimated dietary contributions. We show that a relatively high lipid content in the muscles and livers of some large-bodied terrestrial omnivores do not necessarily have an effect on dietary estimates that rely on carbon stable isotopes. Our results suggest that ethanol storage could be a valuable alternative method for preserving animal tissue prior to stable isotope analysis when freezing or drying is impractical. Nevertheless, further research is needed on the mechanisms that control changes in stable isotope composition in tissues stored in ethanol. We recommend investigating the effects of ethanol on stable isotope values in species and tissues of interest before storing samples in ethanol. |
format |
Other/Unknown Material |
author |
Javornik, Jernej Hopkins, John III Zavadlav, Saša Levanič, Tom Lojen, Sonja Polak, Tomaž Jerina, Klemen |
author_facet |
Javornik, Jernej Hopkins, John III Zavadlav, Saša Levanič, Tom Lojen, Sonja Polak, Tomaž Jerina, Klemen |
author_sort |
Javornik, Jernej |
title |
Effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore |
title_short |
Effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore |
title_full |
Effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore |
title_fullStr |
Effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore |
title_full_unstemmed |
Effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore |
title_sort |
effects of ethanol storage and lipids on stable isotope values in a large mammalian omnivore |
publishDate |
2019 |
url |
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=106175 https://plus.si.cobiss.net/opac7/bib/5301926?lang=sl |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1093/jmammal/gyy187 info:eu-repo/grantAgreement/ARRS/Raziskovalni%20projekti%20-%20temeljni/J4-7362 https://repozitorij.uni-lj.si/IzpisGradiva.php?id=106175 https://plus.si.cobiss.net/opac7/bib/5301926?lang=sl |
op_rights |
info:eu-repo/semantics/embargoedAccess |
op_doi |
https://doi.org/10.1093/jmammal/gyy187 |
container_title |
Journal of Mammalogy |
container_volume |
100 |
container_issue |
1 |
container_start_page |
150 |
op_container_end_page |
157 |
_version_ |
1766231530328293376 |