Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae)
The effects of high PAR (400-700 nm), UVA (315-400 nm), and UVB (280-315 nm) radiation on viability and photosynthesis were investigated for Chaetoceros brevis Schutt. This Antarctic marine diatom was cultivated under low, medium, and high irradiance and nitrate, phosphate, silicate, and iron limita...
Published in: | Journal of Phycology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2005
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Online Access: | https://hdl.handle.net/11370/d4569260-c5a2-4447-8510-b5bffe5ca6ca https://research.rug.nl/en/publications/d4569260-c5a2-4447-8510-b5bffe5ca6ca https://doi.org/10.1111/j.1529-8817.2005.00105.x |
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ftunigroningenpu:oai:pure.rug.nl:publications/d4569260-c5a2-4447-8510-b5bffe5ca6ca 2024-06-02T07:57:27+00:00 Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) van de Poll, WH van Leeuwe, MA Roggeveld, J Buma, AGJ 2005-08 https://hdl.handle.net/11370/d4569260-c5a2-4447-8510-b5bffe5ca6ca https://research.rug.nl/en/publications/d4569260-c5a2-4447-8510-b5bffe5ca6ca https://doi.org/10.1111/j.1529-8817.2005.00105.x eng eng https://research.rug.nl/en/publications/d4569260-c5a2-4447-8510-b5bffe5ca6ca info:eu-repo/semantics/closedAccess van de Poll , WH , van Leeuwe , MA , Roggeveld , J & Buma , AGJ 2005 , ' Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) ' , Journal of Phycology , vol. 41 , no. 4 , pp. 840-850 . https://doi.org/10.1111/j.1529-8817.2005.00105.x Antarctic diatoms flow cytometry irradiance acclimation nutrient limitation oxidative stress photosynthesis pigments UVA radiation UVB radiation viability ULTRAVIOLET-B RADIATION PHOTOSYNTHETIC ENERGY-CONVERSION BIOLOGICAL WEIGHTING FUNCTION SEA-ICE DIATOMS MARINE DIATOM PHAEODACTYLUM-TRICORNUTUM DIADINOXANTHIN CYCLE IRON-LIMITATION SOUTHERN-OCEAN article 2005 ftunigroningenpu https://doi.org/10.1111/j.1529-8817.2005.00105.x 2024-05-07T18:24:10Z The effects of high PAR (400-700 nm), UVA (315-400 nm), and UVB (280-315 nm) radiation on viability and photosynthesis were investigated for Chaetoceros brevis Schutt. This Antarctic marine diatom was cultivated under low, medium, and high irradiance and nitrate, phosphate, silicate, and iron limitation before exposure to a simulated surface irradiance (SSI) treatment, with and without UVB radiation. Light-harvesting and protective pigment composition and PSII parameters were determined before SSI exposure, whereas viability was measured by flow cytometry in combination with a viability stain after the treatment. Recovery of PSII efficiency was measured after 20 h in dim light in a separate experiment. In addition, low and high irradiance acclimated cells were exposed outdoors for 4 h to assess the effects of natural PAR, UVA, and UVB on viability. Low irradiance acclimated cells were particularly sensitive to photo induced viability loss, whereas no viability loss was found after acclimation to high irradiance. Furthermore, nutrient limitation reduced sensitivity to photo induced viability loss, relative to nutrient replete conditions. No additional viability loss was found after UVB exposure. Sunlight exposed cells showed no additional UVB effect on viability, whereas UVA and PAR significantly reduced the viability of low irradiance acclimated cells. Recovery of PSII function was nearly complete in cultures that survived the light treatments. Increased resistance to high irradiance coincided with an increased ratio between protective- and light-harvesting pigments before the SSI treatment, demonstrating the importance of nonphotochemical quenching by diatoxanthin for survival of near-surface irradiance. We conclude that a sudden transfer to high irradiance can be fatal for low irradiance acclimated C. brevis. Article in Journal/Newspaper Antarc* Antarctic Sea ice Southern Ocean University of Groningen research database Antarctic Southern Ocean The Antarctic Journal of Phycology 41 4 840 850 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
Antarctic diatoms flow cytometry irradiance acclimation nutrient limitation oxidative stress photosynthesis pigments UVA radiation UVB radiation viability ULTRAVIOLET-B RADIATION PHOTOSYNTHETIC ENERGY-CONVERSION BIOLOGICAL WEIGHTING FUNCTION SEA-ICE DIATOMS MARINE DIATOM PHAEODACTYLUM-TRICORNUTUM DIADINOXANTHIN CYCLE IRON-LIMITATION SOUTHERN-OCEAN |
spellingShingle |
Antarctic diatoms flow cytometry irradiance acclimation nutrient limitation oxidative stress photosynthesis pigments UVA radiation UVB radiation viability ULTRAVIOLET-B RADIATION PHOTOSYNTHETIC ENERGY-CONVERSION BIOLOGICAL WEIGHTING FUNCTION SEA-ICE DIATOMS MARINE DIATOM PHAEODACTYLUM-TRICORNUTUM DIADINOXANTHIN CYCLE IRON-LIMITATION SOUTHERN-OCEAN van de Poll, WH van Leeuwe, MA Roggeveld, J Buma, AGJ Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) |
topic_facet |
Antarctic diatoms flow cytometry irradiance acclimation nutrient limitation oxidative stress photosynthesis pigments UVA radiation UVB radiation viability ULTRAVIOLET-B RADIATION PHOTOSYNTHETIC ENERGY-CONVERSION BIOLOGICAL WEIGHTING FUNCTION SEA-ICE DIATOMS MARINE DIATOM PHAEODACTYLUM-TRICORNUTUM DIADINOXANTHIN CYCLE IRON-LIMITATION SOUTHERN-OCEAN |
description |
The effects of high PAR (400-700 nm), UVA (315-400 nm), and UVB (280-315 nm) radiation on viability and photosynthesis were investigated for Chaetoceros brevis Schutt. This Antarctic marine diatom was cultivated under low, medium, and high irradiance and nitrate, phosphate, silicate, and iron limitation before exposure to a simulated surface irradiance (SSI) treatment, with and without UVB radiation. Light-harvesting and protective pigment composition and PSII parameters were determined before SSI exposure, whereas viability was measured by flow cytometry in combination with a viability stain after the treatment. Recovery of PSII efficiency was measured after 20 h in dim light in a separate experiment. In addition, low and high irradiance acclimated cells were exposed outdoors for 4 h to assess the effects of natural PAR, UVA, and UVB on viability. Low irradiance acclimated cells were particularly sensitive to photo induced viability loss, whereas no viability loss was found after acclimation to high irradiance. Furthermore, nutrient limitation reduced sensitivity to photo induced viability loss, relative to nutrient replete conditions. No additional viability loss was found after UVB exposure. Sunlight exposed cells showed no additional UVB effect on viability, whereas UVA and PAR significantly reduced the viability of low irradiance acclimated cells. Recovery of PSII function was nearly complete in cultures that survived the light treatments. Increased resistance to high irradiance coincided with an increased ratio between protective- and light-harvesting pigments before the SSI treatment, demonstrating the importance of nonphotochemical quenching by diatoxanthin for survival of near-surface irradiance. We conclude that a sudden transfer to high irradiance can be fatal for low irradiance acclimated C. brevis. |
format |
Article in Journal/Newspaper |
author |
van de Poll, WH van Leeuwe, MA Roggeveld, J Buma, AGJ |
author_facet |
van de Poll, WH van Leeuwe, MA Roggeveld, J Buma, AGJ |
author_sort |
van de Poll, WH |
title |
Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) |
title_short |
Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) |
title_full |
Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) |
title_fullStr |
Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) |
title_full_unstemmed |
Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) |
title_sort |
nutrient limitation and high irradiance acclimation reduce par and uv-induced viability loss in the antarctic diatom chaetoceros brevis (bacillariophyceae) |
publishDate |
2005 |
url |
https://hdl.handle.net/11370/d4569260-c5a2-4447-8510-b5bffe5ca6ca https://research.rug.nl/en/publications/d4569260-c5a2-4447-8510-b5bffe5ca6ca https://doi.org/10.1111/j.1529-8817.2005.00105.x |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Sea ice Southern Ocean |
op_source |
van de Poll , WH , van Leeuwe , MA , Roggeveld , J & Buma , AGJ 2005 , ' Nutrient limitation and high irradiance acclimation reduce PAR and UV-induced viability loss in the Antarctic diatom Chaetoceros brevis (Bacillariophyceae) ' , Journal of Phycology , vol. 41 , no. 4 , pp. 840-850 . https://doi.org/10.1111/j.1529-8817.2005.00105.x |
op_relation |
https://research.rug.nl/en/publications/d4569260-c5a2-4447-8510-b5bffe5ca6ca |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/j.1529-8817.2005.00105.x |
container_title |
Journal of Phycology |
container_volume |
41 |
container_issue |
4 |
container_start_page |
840 |
op_container_end_page |
850 |
_version_ |
1800740612983488512 |