Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions
Accumulation and removal of UVBR (ultraviolet-B radiation: 280 to 315 nm)-induced DNA damage (cyclobutane pyrimidine dimers; CPDs) were studied in 2 size fractions (0.2 to 0.8 and 0.8 to 10 mum) of natural populations of tropical marine bacterio- and phytoplankton off Curacao, Netherlands Antilles....
Published in: | Aquatic Microbial Ecology |
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Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2001
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Online Access: | https://hdl.handle.net/11370/cbd04bc7-4178-4481-b30c-c13c0cfe1748 https://research.rug.nl/en/publications/cbd04bc7-4178-4481-b30c-c13c0cfe1748 https://doi.org/10.3354/ame024265 https://pure.rug.nl/ws/files/67173481/a024p265.pdf |
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ftunigroningenpu:oai:pure.rug.nl:publications/cbd04bc7-4178-4481-b30c-c13c0cfe1748 2024-06-02T07:57:16+00:00 Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions Boelen, Peter Veldhuis, Marcel J W Buma, Anita G J 2001-07-18 application/pdf https://hdl.handle.net/11370/cbd04bc7-4178-4481-b30c-c13c0cfe1748 https://research.rug.nl/en/publications/cbd04bc7-4178-4481-b30c-c13c0cfe1748 https://doi.org/10.3354/ame024265 https://pure.rug.nl/ws/files/67173481/a024p265.pdf eng eng https://research.rug.nl/en/publications/cbd04bc7-4178-4481-b30c-c13c0cfe1748 info:eu-repo/semantics/openAccess Boelen , P , Veldhuis , M J W & Buma , A G J 2001 , ' Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions ' , Aquatic Microbial Ecology , vol. 24 , no. 3 , pp. 265-274 . https://doi.org/10.3354/ame024265 cyclobutane pyrimidine dimers DNA damage DNA repair Caribbean Sea marine bacteria mixing phytoplankton ultraviolet-B radiation UVBR ANTARCTIC PHYTOPLANKTON BACTERIOPLANKTON WATERS COMMUNITIES CELLS INHIBITION ATLANTIC SURFACE OCEAN article 2001 ftunigroningenpu https://doi.org/10.3354/ame024265 2024-05-07T17:46:31Z Accumulation and removal of UVBR (ultraviolet-B radiation: 280 to 315 nm)-induced DNA damage (cyclobutane pyrimidine dimers; CPDs) were studied in 2 size fractions (0.2 to 0.8 and 0.8 to 10 mum) of natural populations of tropical marine bacterio- and phytoplankton off Curacao, Netherlands Antilles. Plankton was either directly sampled from the surface layer (mixed situation), or incubated in UVR-transparent bags at 2 water depths (simulated non-mixed situation). A DNA dosimeter was used to measure biologically effective UVBR doses as well as attenuation of biologically effective radiation in the surface layer. A significant difference in CPD level between the 2 plankton size fractions could not be measured. In the mixed as well as in the non-mixed situation, DNA damage was accumulated between 09:00 and 14:00 h irrespective of the variability in incident UVBR. Surface layer samplings on consecutive days showed a clear diel pattern of increase and decrease, indicating that, in the field, DNA damage is repaired or diluted during the afternoon or at night. No significant repair of DNA damage could be measured in samples that were incubated during the afternoon at 0.2 or 10 m depth. The UVBR sensitivity of surface-incubated plankton was expressed as the ratio between damage induced in the cells to damage induced in (unshielded and unrepaired) bare DNA. In both plankton size classes this ratio fluctuated around 0.32, In conclusion, the results show that plankton cells present in the clear waters off Curacao undergo UVBR stress despite the fact that they are subjected to vertical mixing. No significant difference in CPD levels between 2 size fractions of plankton could be measured, suggesting that in this plankton population other aspects, e.g. repair capacities or cell morphology, were more important than cell size. Article in Journal/Newspaper Antarc* Antarctic University of Groningen research database Antarctic Aquatic Microbial Ecology 24 265 274 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
cyclobutane pyrimidine dimers DNA damage DNA repair Caribbean Sea marine bacteria mixing phytoplankton ultraviolet-B radiation UVBR ANTARCTIC PHYTOPLANKTON BACTERIOPLANKTON WATERS COMMUNITIES CELLS INHIBITION ATLANTIC SURFACE OCEAN |
spellingShingle |
cyclobutane pyrimidine dimers DNA damage DNA repair Caribbean Sea marine bacteria mixing phytoplankton ultraviolet-B radiation UVBR ANTARCTIC PHYTOPLANKTON BACTERIOPLANKTON WATERS COMMUNITIES CELLS INHIBITION ATLANTIC SURFACE OCEAN Boelen, Peter Veldhuis, Marcel J W Buma, Anita G J Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions |
topic_facet |
cyclobutane pyrimidine dimers DNA damage DNA repair Caribbean Sea marine bacteria mixing phytoplankton ultraviolet-B radiation UVBR ANTARCTIC PHYTOPLANKTON BACTERIOPLANKTON WATERS COMMUNITIES CELLS INHIBITION ATLANTIC SURFACE OCEAN |
description |
Accumulation and removal of UVBR (ultraviolet-B radiation: 280 to 315 nm)-induced DNA damage (cyclobutane pyrimidine dimers; CPDs) were studied in 2 size fractions (0.2 to 0.8 and 0.8 to 10 mum) of natural populations of tropical marine bacterio- and phytoplankton off Curacao, Netherlands Antilles. Plankton was either directly sampled from the surface layer (mixed situation), or incubated in UVR-transparent bags at 2 water depths (simulated non-mixed situation). A DNA dosimeter was used to measure biologically effective UVBR doses as well as attenuation of biologically effective radiation in the surface layer. A significant difference in CPD level between the 2 plankton size fractions could not be measured. In the mixed as well as in the non-mixed situation, DNA damage was accumulated between 09:00 and 14:00 h irrespective of the variability in incident UVBR. Surface layer samplings on consecutive days showed a clear diel pattern of increase and decrease, indicating that, in the field, DNA damage is repaired or diluted during the afternoon or at night. No significant repair of DNA damage could be measured in samples that were incubated during the afternoon at 0.2 or 10 m depth. The UVBR sensitivity of surface-incubated plankton was expressed as the ratio between damage induced in the cells to damage induced in (unshielded and unrepaired) bare DNA. In both plankton size classes this ratio fluctuated around 0.32, In conclusion, the results show that plankton cells present in the clear waters off Curacao undergo UVBR stress despite the fact that they are subjected to vertical mixing. No significant difference in CPD levels between 2 size fractions of plankton could be measured, suggesting that in this plankton population other aspects, e.g. repair capacities or cell morphology, were more important than cell size. |
format |
Article in Journal/Newspaper |
author |
Boelen, Peter Veldhuis, Marcel J W Buma, Anita G J |
author_facet |
Boelen, Peter Veldhuis, Marcel J W Buma, Anita G J |
author_sort |
Boelen, Peter |
title |
Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions |
title_short |
Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions |
title_full |
Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions |
title_fullStr |
Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions |
title_full_unstemmed |
Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions |
title_sort |
accumulation and removal of uvbr-induced dna damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions |
publishDate |
2001 |
url |
https://hdl.handle.net/11370/cbd04bc7-4178-4481-b30c-c13c0cfe1748 https://research.rug.nl/en/publications/cbd04bc7-4178-4481-b30c-c13c0cfe1748 https://doi.org/10.3354/ame024265 https://pure.rug.nl/ws/files/67173481/a024p265.pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Boelen , P , Veldhuis , M J W & Buma , A G J 2001 , ' Accumulation and removal of UVBR-induced DNA damage in marine tropical plankton subjected to mixed and simulated non-mixed conditions ' , Aquatic Microbial Ecology , vol. 24 , no. 3 , pp. 265-274 . https://doi.org/10.3354/ame024265 |
op_relation |
https://research.rug.nl/en/publications/cbd04bc7-4178-4481-b30c-c13c0cfe1748 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3354/ame024265 |
container_title |
Aquatic Microbial Ecology |
container_volume |
24 |
container_start_page |
265 |
op_container_end_page |
274 |
_version_ |
1800740357109972992 |