Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci
Between three and six tri- and tetranucleotide repeat microsatellite loci were analyzed in 3720 samples collected from four different species of baleen whales. Ten of the 18 species/locus combinations had imperfect allele arrays, i.e., some alleles differed in length by other than simple integer mul...
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ftunigroningenpu:oai:pure.rug.nl:publications/bd09b87b-1dc6-4078-be18-34e5a06743a5 2024-06-02T08:04:00+00:00 Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci Palsboll, PJ Berube, M Jorgensen, H 1999-01 https://hdl.handle.net/11370/bd09b87b-1dc6-4078-be18-34e5a06743a5 https://research.rug.nl/en/publications/bd09b87b-1dc6-4078-be18-34e5a06743a5 eng eng https://research.rug.nl/en/publications/bd09b87b-1dc6-4078-be18-34e5a06743a5 info:eu-repo/semantics/restrictedAccess Palsboll , PJ , Berube , M & Jorgensen , H 1999 , ' Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci ' , Genetics , vol. 151 , no. 1 , pp. 285-296 . STEPWISE MUTATION MODEL POLYMERASE CHAIN-REACTION ALLELE FREQUENCIES GENETIC DISTANCES DIRECTIONAL EVOLUTION HUMPBACK WHALES TANDEM REPEATS DNA POPULATIONS SIZE article 1999 ftunigroningenpu 2024-05-07T19:28:23Z Between three and six tri- and tetranucleotide repeat microsatellite loci were analyzed in 3720 samples collected from four different species of baleen whales. Ten of the 18 species/locus combinations had imperfect allele arrays, i.e., some alleles differed in length by other than simple integer multiples of the basic repeat length. The estimate of the average number of alleles and heterozygosity was higher at loci with imperfect allele arrays relative to those with perfect allele arrays. Nucleotide sequences of 23 different alleles at one tetranucleotide repeat microsatellite locus in fin whales, Balaenoptera physalus, and humpback whales, Megaptera novaeangliae, revealed sequence changes including perfect repeats only, multiple repeats, and partial repeats. The relative rate of the latter two categories of mutation was estimated at 0.024 of the mutation rate involving perfect repeats only. It is hypothesized that single-strand slippage of partial repeats may provide a mechanism for counteracting the continuous expansion of microsatellite loci, which is the logical consequence of recent reports demonstrating directional mutations. Partial-repeat mutations introduce imperfections in the repeat array, which subsequently could reduce the rate of single-strand slippage. Limited computer simulations confirmed this predicted effect of partial-repeat mutations. Article in Journal/Newspaper Balaenoptera physalus baleen whales Megaptera novaeangliae University of Groningen research database |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
STEPWISE MUTATION MODEL POLYMERASE CHAIN-REACTION ALLELE FREQUENCIES GENETIC DISTANCES DIRECTIONAL EVOLUTION HUMPBACK WHALES TANDEM REPEATS DNA POPULATIONS SIZE |
spellingShingle |
STEPWISE MUTATION MODEL POLYMERASE CHAIN-REACTION ALLELE FREQUENCIES GENETIC DISTANCES DIRECTIONAL EVOLUTION HUMPBACK WHALES TANDEM REPEATS DNA POPULATIONS SIZE Palsboll, PJ Berube, M Jorgensen, H Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci |
topic_facet |
STEPWISE MUTATION MODEL POLYMERASE CHAIN-REACTION ALLELE FREQUENCIES GENETIC DISTANCES DIRECTIONAL EVOLUTION HUMPBACK WHALES TANDEM REPEATS DNA POPULATIONS SIZE |
description |
Between three and six tri- and tetranucleotide repeat microsatellite loci were analyzed in 3720 samples collected from four different species of baleen whales. Ten of the 18 species/locus combinations had imperfect allele arrays, i.e., some alleles differed in length by other than simple integer multiples of the basic repeat length. The estimate of the average number of alleles and heterozygosity was higher at loci with imperfect allele arrays relative to those with perfect allele arrays. Nucleotide sequences of 23 different alleles at one tetranucleotide repeat microsatellite locus in fin whales, Balaenoptera physalus, and humpback whales, Megaptera novaeangliae, revealed sequence changes including perfect repeats only, multiple repeats, and partial repeats. The relative rate of the latter two categories of mutation was estimated at 0.024 of the mutation rate involving perfect repeats only. It is hypothesized that single-strand slippage of partial repeats may provide a mechanism for counteracting the continuous expansion of microsatellite loci, which is the logical consequence of recent reports demonstrating directional mutations. Partial-repeat mutations introduce imperfections in the repeat array, which subsequently could reduce the rate of single-strand slippage. Limited computer simulations confirmed this predicted effect of partial-repeat mutations. |
format |
Article in Journal/Newspaper |
author |
Palsboll, PJ Berube, M Jorgensen, H |
author_facet |
Palsboll, PJ Berube, M Jorgensen, H |
author_sort |
Palsboll, PJ |
title |
Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci |
title_short |
Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci |
title_full |
Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci |
title_fullStr |
Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci |
title_full_unstemmed |
Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci |
title_sort |
multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci |
publishDate |
1999 |
url |
https://hdl.handle.net/11370/bd09b87b-1dc6-4078-be18-34e5a06743a5 https://research.rug.nl/en/publications/bd09b87b-1dc6-4078-be18-34e5a06743a5 |
genre |
Balaenoptera physalus baleen whales Megaptera novaeangliae |
genre_facet |
Balaenoptera physalus baleen whales Megaptera novaeangliae |
op_source |
Palsboll , PJ , Berube , M & Jorgensen , H 1999 , ' Multiple levels of single-strand slippage at cetacean tri- and tetranucleotide repeat microsatellite loci ' , Genetics , vol. 151 , no. 1 , pp. 285-296 . |
op_relation |
https://research.rug.nl/en/publications/bd09b87b-1dc6-4078-be18-34e5a06743a5 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
_version_ |
1800748613208375296 |