Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification
Antarctic coastal waters undergo major physical alterations during summer. Increased temperatures induce sea-ice melting and glacial melt water input, leading to strong stratification of the upper water column. We investigated the composition of micro-eukaryotic and bacterial communities in Ryder Ba...
Published in: | FEMS Microbiology Ecology |
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2011
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Online Access: | https://hdl.handle.net/11370/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 https://research.rug.nl/en/publications/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 https://doi.org/10.1111/j.1574-6941.2011.01062.x |
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ftunigroningenpu:oai:pure.rug.nl:publications/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 2024-06-02T07:56:52+00:00 Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification Piquet, Anouk M. -T. Bolhuis, Henk Meredith, Michael P. Buma, Anita G. J. 2011-06 https://hdl.handle.net/11370/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 https://research.rug.nl/en/publications/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 https://doi.org/10.1111/j.1574-6941.2011.01062.x eng eng https://research.rug.nl/en/publications/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 info:eu-repo/semantics/closedAccess Piquet , A M -T , Bolhuis , H , Meredith , M P & Buma , A G J 2011 , ' Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification ' , FEMS Microbial Ecology , vol. 76 , no. 3 , pp. 413-427 . https://doi.org/10.1111/j.1574-6941.2011.01062.x Antarctic Peninsula 18S rRNA gene 16S rRNA gene bacterioplankton DGGE sequencing GRADIENT GEL-ELECTROPHORESIS 16S RIBOSOMAL-RNA SOUTHERN-OCEAN PHYTOPLANKTON BIOMASS CLASS FLAVOBACTERIA UV-RADIATION DEEP-SEA DIVERSITY ASSEMBLAGES BACTERIAL article 2011 ftunigroningenpu https://doi.org/10.1111/j.1574-6941.2011.01062.x 2024-05-07T18:49:19Z Antarctic coastal waters undergo major physical alterations during summer. Increased temperatures induce sea-ice melting and glacial melt water input, leading to strong stratification of the upper water column. We investigated the composition of micro-eukaryotic and bacterial communities in Ryder Bay, Antarctic Peninsula, during and after summertime melt water stratification, applying community fingerprinting (denaturing gradient gel electrophoresis) and sequencing analysis of partial 18S and 16S rRNA genes. Community fingerprinting of the eukaryotic community revealed two major patterns, coinciding with a period of melt water stratification, followed by a period characterized by regular wind-induced breakdown of surface stratification. During the first stratified period, we observed depth-related differences in eukaryotic fingerprints while differences in bacterial fingerprints were weak. Wind-induced breakdown of the melt water layer caused a shift in the eukaryotic community from an Actinocyclus sp.- to a Thalassiosira sp.-dominated community. In addition, a distinct transition in the bacterial community was found, but with a few days' delay, suggesting a response to the changes in the eukaryotic community rather than to the mixing event itself. Sequence analysis revealed a shift from an Alpha- and Gammaproteobacteria to a Cytophaga-Flavobacterium-Bacteroides-dominated community under mixed conditions. Our results show that melt water stratification and the transition to nonstabilized Antarctic surface waters may have an impact not only on micro-eukaryotic but also bacterial community composition. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Sea ice Southern Ocean University of Groningen research database Antarctic Southern Ocean Antarctic Peninsula Ryder ENVELOPE(-68.333,-68.333,-67.566,-67.566) Ryder Bay ENVELOPE(-68.333,-68.333,-67.567,-67.567) FEMS Microbiology Ecology 76 3 413 427 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
Antarctic Peninsula 18S rRNA gene 16S rRNA gene bacterioplankton DGGE sequencing GRADIENT GEL-ELECTROPHORESIS 16S RIBOSOMAL-RNA SOUTHERN-OCEAN PHYTOPLANKTON BIOMASS CLASS FLAVOBACTERIA UV-RADIATION DEEP-SEA DIVERSITY ASSEMBLAGES BACTERIAL |
spellingShingle |
Antarctic Peninsula 18S rRNA gene 16S rRNA gene bacterioplankton DGGE sequencing GRADIENT GEL-ELECTROPHORESIS 16S RIBOSOMAL-RNA SOUTHERN-OCEAN PHYTOPLANKTON BIOMASS CLASS FLAVOBACTERIA UV-RADIATION DEEP-SEA DIVERSITY ASSEMBLAGES BACTERIAL Piquet, Anouk M. -T. Bolhuis, Henk Meredith, Michael P. Buma, Anita G. J. Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification |
topic_facet |
Antarctic Peninsula 18S rRNA gene 16S rRNA gene bacterioplankton DGGE sequencing GRADIENT GEL-ELECTROPHORESIS 16S RIBOSOMAL-RNA SOUTHERN-OCEAN PHYTOPLANKTON BIOMASS CLASS FLAVOBACTERIA UV-RADIATION DEEP-SEA DIVERSITY ASSEMBLAGES BACTERIAL |
description |
Antarctic coastal waters undergo major physical alterations during summer. Increased temperatures induce sea-ice melting and glacial melt water input, leading to strong stratification of the upper water column. We investigated the composition of micro-eukaryotic and bacterial communities in Ryder Bay, Antarctic Peninsula, during and after summertime melt water stratification, applying community fingerprinting (denaturing gradient gel electrophoresis) and sequencing analysis of partial 18S and 16S rRNA genes. Community fingerprinting of the eukaryotic community revealed two major patterns, coinciding with a period of melt water stratification, followed by a period characterized by regular wind-induced breakdown of surface stratification. During the first stratified period, we observed depth-related differences in eukaryotic fingerprints while differences in bacterial fingerprints were weak. Wind-induced breakdown of the melt water layer caused a shift in the eukaryotic community from an Actinocyclus sp.- to a Thalassiosira sp.-dominated community. In addition, a distinct transition in the bacterial community was found, but with a few days' delay, suggesting a response to the changes in the eukaryotic community rather than to the mixing event itself. Sequence analysis revealed a shift from an Alpha- and Gammaproteobacteria to a Cytophaga-Flavobacterium-Bacteroides-dominated community under mixed conditions. Our results show that melt water stratification and the transition to nonstabilized Antarctic surface waters may have an impact not only on micro-eukaryotic but also bacterial community composition. |
format |
Article in Journal/Newspaper |
author |
Piquet, Anouk M. -T. Bolhuis, Henk Meredith, Michael P. Buma, Anita G. J. |
author_facet |
Piquet, Anouk M. -T. Bolhuis, Henk Meredith, Michael P. Buma, Anita G. J. |
author_sort |
Piquet, Anouk M. -T. |
title |
Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification |
title_short |
Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification |
title_full |
Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification |
title_fullStr |
Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification |
title_full_unstemmed |
Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification |
title_sort |
shifts in coastal antarctic marine microbial communities during and after melt water-related surface stratification |
publishDate |
2011 |
url |
https://hdl.handle.net/11370/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 https://research.rug.nl/en/publications/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 https://doi.org/10.1111/j.1574-6941.2011.01062.x |
long_lat |
ENVELOPE(-68.333,-68.333,-67.566,-67.566) ENVELOPE(-68.333,-68.333,-67.567,-67.567) |
geographic |
Antarctic Southern Ocean Antarctic Peninsula Ryder Ryder Bay |
geographic_facet |
Antarctic Southern Ocean Antarctic Peninsula Ryder Ryder Bay |
genre |
Antarc* Antarctic Antarctic Peninsula Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Sea ice Southern Ocean |
op_source |
Piquet , A M -T , Bolhuis , H , Meredith , M P & Buma , A G J 2011 , ' Shifts in coastal Antarctic marine microbial communities during and after melt water-related surface stratification ' , FEMS Microbial Ecology , vol. 76 , no. 3 , pp. 413-427 . https://doi.org/10.1111/j.1574-6941.2011.01062.x |
op_relation |
https://research.rug.nl/en/publications/bc9dfb72-7a1e-4948-aa42-12ed32d8b849 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/j.1574-6941.2011.01062.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
76 |
container_issue |
3 |
container_start_page |
413 |
op_container_end_page |
427 |
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1800759704692981760 |