Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen)
Krossfjorden and Kongsfjorden are Arctic fjords on the western side of Spitsbergen. These fjords share a common mouth to the open sea, and both are influenced by the input of sediment-rich glacial meltwater leading to decreased surface salinity, increased turbidity and decreased light penetration du...
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Language: | English |
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2010
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Online Access: | https://hdl.handle.net/11370/b4dd8928-ba9d-464c-9e27-650dafc5f864 https://research.rug.nl/en/publications/b4dd8928-ba9d-464c-9e27-650dafc5f864 https://doi.org/10.1007/s00300-010-0841-9 https://pure.rug.nl/ws/files/171565237/Variability_of_protistan_and_bacterial_communities_in_two_Arctic_fjords.pdf |
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ftunigroningenpu:oai:pure.rug.nl:publications/b4dd8928-ba9d-464c-9e27-650dafc5f864 2024-06-23T07:48:33+00:00 Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) Piquet, A. M. -T. Scheepens, J. F. Bolhuis, H. Wiencke, C. Buma, A. G. J. 2010-11 application/pdf https://hdl.handle.net/11370/b4dd8928-ba9d-464c-9e27-650dafc5f864 https://research.rug.nl/en/publications/b4dd8928-ba9d-464c-9e27-650dafc5f864 https://doi.org/10.1007/s00300-010-0841-9 https://pure.rug.nl/ws/files/171565237/Variability_of_protistan_and_bacterial_communities_in_two_Arctic_fjords.pdf eng eng https://research.rug.nl/en/publications/b4dd8928-ba9d-464c-9e27-650dafc5f864 info:eu-repo/semantics/openAccess Piquet , A M -T , Scheepens , J F , Bolhuis , H , Wiencke , C & Buma , A G J 2010 , ' Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) ' , Polar Biology , vol. 33 , no. 11 , pp. 1521-1536 . https://doi.org/10.1007/s00300-010-0841-9 Marine microbes DGGE Sequencing Climate change Meltwater Polar Kongsfjorden Krossfjorden GRADIENT GEL-ELECTROPHORESIS 16S RIBOSOMAL-RNA SOUTHERN-OCEAN HETEROTROPHIC PRODUCTION PHYSICAL-ENVIRONMENT PACK ICE DIVERSITY MARINE BACTERIOPLANKTON PHYTOPLANKTON article 2010 ftunigroningenpu https://doi.org/10.1007/s00300-010-0841-9 2024-06-03T16:23:25Z Krossfjorden and Kongsfjorden are Arctic fjords on the western side of Spitsbergen. These fjords share a common mouth to the open sea, and both are influenced by the input of sediment-rich glacial meltwater leading to decreased surface salinity, increased turbidity and decreased light penetration during summer. Earlier classical taxonomic studies had described the pelagic protistan composition of the Kongsfjorden during summer, revealing the dominance of flagellates of often unresolved taxonomic origin. Only little information existed on microbial eukaryote composition of the Krossfjorden as well as the bacterial composition of both fjords. The aim of the present study was to analyze and compare surface summertime protistan and bacterial communities in both fjords, using molecular approaches (16S and 18S rRNA DGGE, sequencing). Samples were collected three times a week from the central Kongsfjorden over a 1-month period. Additionally, 10 marine and 2 freshwater sites were sampled within a 1-week period in both Kongsfjorden and Krossfjorden. The central Kongsfjorden revealed a relatively stable protistan community over time with dinoflagellates, chlorophytes and small heterotrophs dominating. In contrast, the bacterial community varied over time and appeared to be correlated with the inflow of glacial meltwater. The Kongsfjorden and Krossfjorden were found to harbor distinctive bacterial and eukaryotic communities. We speculate that differences in glacial meltwater composition and fjord bathymetry affect the surface water properties and therefore the observed spatial variability in the community fingerprints. Article in Journal/Newspaper Arctic Arctic Climate change Kongsfjord* Kongsfjorden Krossfjord* Phytoplankton Polar Biology Southern Ocean Spitsbergen University of Groningen research database Arctic Krossfjorden ENVELOPE(11.742,11.742,79.141,79.141) Southern Ocean Polar Biology 33 11 1521 1536 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
Marine microbes DGGE Sequencing Climate change Meltwater Polar Kongsfjorden Krossfjorden GRADIENT GEL-ELECTROPHORESIS 16S RIBOSOMAL-RNA SOUTHERN-OCEAN HETEROTROPHIC PRODUCTION PHYSICAL-ENVIRONMENT PACK ICE DIVERSITY MARINE BACTERIOPLANKTON PHYTOPLANKTON |
spellingShingle |
Marine microbes DGGE Sequencing Climate change Meltwater Polar Kongsfjorden Krossfjorden GRADIENT GEL-ELECTROPHORESIS 16S RIBOSOMAL-RNA SOUTHERN-OCEAN HETEROTROPHIC PRODUCTION PHYSICAL-ENVIRONMENT PACK ICE DIVERSITY MARINE BACTERIOPLANKTON PHYTOPLANKTON Piquet, A. M. -T. Scheepens, J. F. Bolhuis, H. Wiencke, C. Buma, A. G. J. Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) |
topic_facet |
Marine microbes DGGE Sequencing Climate change Meltwater Polar Kongsfjorden Krossfjorden GRADIENT GEL-ELECTROPHORESIS 16S RIBOSOMAL-RNA SOUTHERN-OCEAN HETEROTROPHIC PRODUCTION PHYSICAL-ENVIRONMENT PACK ICE DIVERSITY MARINE BACTERIOPLANKTON PHYTOPLANKTON |
description |
Krossfjorden and Kongsfjorden are Arctic fjords on the western side of Spitsbergen. These fjords share a common mouth to the open sea, and both are influenced by the input of sediment-rich glacial meltwater leading to decreased surface salinity, increased turbidity and decreased light penetration during summer. Earlier classical taxonomic studies had described the pelagic protistan composition of the Kongsfjorden during summer, revealing the dominance of flagellates of often unresolved taxonomic origin. Only little information existed on microbial eukaryote composition of the Krossfjorden as well as the bacterial composition of both fjords. The aim of the present study was to analyze and compare surface summertime protistan and bacterial communities in both fjords, using molecular approaches (16S and 18S rRNA DGGE, sequencing). Samples were collected three times a week from the central Kongsfjorden over a 1-month period. Additionally, 10 marine and 2 freshwater sites were sampled within a 1-week period in both Kongsfjorden and Krossfjorden. The central Kongsfjorden revealed a relatively stable protistan community over time with dinoflagellates, chlorophytes and small heterotrophs dominating. In contrast, the bacterial community varied over time and appeared to be correlated with the inflow of glacial meltwater. The Kongsfjorden and Krossfjorden were found to harbor distinctive bacterial and eukaryotic communities. We speculate that differences in glacial meltwater composition and fjord bathymetry affect the surface water properties and therefore the observed spatial variability in the community fingerprints. |
format |
Article in Journal/Newspaper |
author |
Piquet, A. M. -T. Scheepens, J. F. Bolhuis, H. Wiencke, C. Buma, A. G. J. |
author_facet |
Piquet, A. M. -T. Scheepens, J. F. Bolhuis, H. Wiencke, C. Buma, A. G. J. |
author_sort |
Piquet, A. M. -T. |
title |
Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) |
title_short |
Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) |
title_full |
Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) |
title_fullStr |
Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) |
title_full_unstemmed |
Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) |
title_sort |
variability of protistan and bacterial communities in two arctic fjords (spitsbergen) |
publishDate |
2010 |
url |
https://hdl.handle.net/11370/b4dd8928-ba9d-464c-9e27-650dafc5f864 https://research.rug.nl/en/publications/b4dd8928-ba9d-464c-9e27-650dafc5f864 https://doi.org/10.1007/s00300-010-0841-9 https://pure.rug.nl/ws/files/171565237/Variability_of_protistan_and_bacterial_communities_in_two_Arctic_fjords.pdf |
long_lat |
ENVELOPE(11.742,11.742,79.141,79.141) |
geographic |
Arctic Krossfjorden Southern Ocean |
geographic_facet |
Arctic Krossfjorden Southern Ocean |
genre |
Arctic Arctic Climate change Kongsfjord* Kongsfjorden Krossfjord* Phytoplankton Polar Biology Southern Ocean Spitsbergen |
genre_facet |
Arctic Arctic Climate change Kongsfjord* Kongsfjorden Krossfjord* Phytoplankton Polar Biology Southern Ocean Spitsbergen |
op_source |
Piquet , A M -T , Scheepens , J F , Bolhuis , H , Wiencke , C & Buma , A G J 2010 , ' Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen) ' , Polar Biology , vol. 33 , no. 11 , pp. 1521-1536 . https://doi.org/10.1007/s00300-010-0841-9 |
op_relation |
https://research.rug.nl/en/publications/b4dd8928-ba9d-464c-9e27-650dafc5f864 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00300-010-0841-9 |
container_title |
Polar Biology |
container_volume |
33 |
container_issue |
11 |
container_start_page |
1521 |
op_container_end_page |
1536 |
_version_ |
1802638921360736256 |