Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae):I. Intracellular DMSP concentrations
Iron is essential for phytoplankton growth, as it is involved in many metabolic processes. It controls photosynthesis as well as many enzymatic processes. As such, iron affects the cell's energy supply and contributes to the assimilation of carbon and nitrogen. To determine whether iron limitat...
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ftunigroningenpu:oai:pure.rug.nl:publications/a428cbd5-a866-4b1e-837b-6d92e47c4cae 2024-06-23T07:47:52+00:00 Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae):I. Intracellular DMSP concentrations Stefels, J van Leeuwe, M A 1998-06 https://hdl.handle.net/11370/a428cbd5-a866-4b1e-837b-6d92e47c4cae https://research.rug.nl/en/publications/a428cbd5-a866-4b1e-837b-6d92e47c4cae eng eng https://research.rug.nl/en/publications/a428cbd5-a866-4b1e-837b-6d92e47c4cae info:eu-repo/semantics/restrictedAccess Stefels , J & van Leeuwe , M A 1998 , ' Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae) : I. Intracellular DMSP concentrations ' , Journal of Phycology , vol. 34 , no. 3 , pp. 486-495 . Antarctic Phaeocystis iron light biochemical composition dimethylsulfoniopropionate DMSP TETRASELMIS-SUBCORDIFORMIS STEIN PHOTOSYNTHETIC ENERGY-CONVERSION EQUATORIAL PACIFIC-OCEAN MARINE EUKARYOTIC ALGAE PHYTOPLANKTON PHOTOSYNTHESIS CELL-VOLUME BETA-DIMETHYLSULPHONIOPROPIONATE CARBOHYDRATE SOLUTES QUATERNARY AMMONIUM NITROGEN LIMITATION article 1998 ftunigroningenpu 2024-06-03T16:11:09Z Iron is essential for phytoplankton growth, as it is involved in many metabolic processes. It controls photosynthesis as well as many enzymatic processes. As such, iron affects the cell's energy supply and contributes to the assimilation of carbon and nitrogen. To determine whether iron limitation would result in energy stress or induced nitrogen deficiency, an Antarctic Phaeocystis sp. (Prymnesiophyceae) strain was studied for its biochemical composition, with the main emphasis on intracellular production of dimethylsulfoniopropionate (DMSP). DMSP is suggested to replace nitrogen containing solutes under conditions of nitrogen deficiency. Batch cultures of Antarctic Phaeocystis sp. were grown under iron-rich and iron-poor conditions and simultaneously subjected to high and low light intensities. Iron depletion induced chlorosis and suppressed growth rates as well as the maximum yield of the cultures; these effects were reinforced by low light intensities. Cell volumes were strongly reduced under iron-limited conditions. However, this reduction in cell volume was accompanied by a reduced DMSP content entry in cultures experiencing low light intensities. Under high light conditions, no reduction of DMSP was observed; hence, intracellular DMSP concentrations increased. These observations are discussed relative to carbon and nitrogen metabolism and the biosynthetic pathway of DMSP. It is argued that under high light, low iron conditions, the cells were bordering on nitrogen deficiency induced by iron limitation, whereas under low light, low iron conditions, the cells were energy limited resulting in overall suppressed metabolic rates. Between treatments, DMSP to chlorophyll-a ratios varied by a factor of 5, demonstrating the dependence of this parameter on the physiological state of the cell. Article in Journal/Newspaper Antarc* Antarctic University of Groningen research database Antarctic Pacific |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
Antarctic Phaeocystis iron light biochemical composition dimethylsulfoniopropionate DMSP TETRASELMIS-SUBCORDIFORMIS STEIN PHOTOSYNTHETIC ENERGY-CONVERSION EQUATORIAL PACIFIC-OCEAN MARINE EUKARYOTIC ALGAE PHYTOPLANKTON PHOTOSYNTHESIS CELL-VOLUME BETA-DIMETHYLSULPHONIOPROPIONATE CARBOHYDRATE SOLUTES QUATERNARY AMMONIUM NITROGEN LIMITATION |
spellingShingle |
Antarctic Phaeocystis iron light biochemical composition dimethylsulfoniopropionate DMSP TETRASELMIS-SUBCORDIFORMIS STEIN PHOTOSYNTHETIC ENERGY-CONVERSION EQUATORIAL PACIFIC-OCEAN MARINE EUKARYOTIC ALGAE PHYTOPLANKTON PHOTOSYNTHESIS CELL-VOLUME BETA-DIMETHYLSULPHONIOPROPIONATE CARBOHYDRATE SOLUTES QUATERNARY AMMONIUM NITROGEN LIMITATION Stefels, J van Leeuwe, M A Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae):I. Intracellular DMSP concentrations |
topic_facet |
Antarctic Phaeocystis iron light biochemical composition dimethylsulfoniopropionate DMSP TETRASELMIS-SUBCORDIFORMIS STEIN PHOTOSYNTHETIC ENERGY-CONVERSION EQUATORIAL PACIFIC-OCEAN MARINE EUKARYOTIC ALGAE PHYTOPLANKTON PHOTOSYNTHESIS CELL-VOLUME BETA-DIMETHYLSULPHONIOPROPIONATE CARBOHYDRATE SOLUTES QUATERNARY AMMONIUM NITROGEN LIMITATION |
description |
Iron is essential for phytoplankton growth, as it is involved in many metabolic processes. It controls photosynthesis as well as many enzymatic processes. As such, iron affects the cell's energy supply and contributes to the assimilation of carbon and nitrogen. To determine whether iron limitation would result in energy stress or induced nitrogen deficiency, an Antarctic Phaeocystis sp. (Prymnesiophyceae) strain was studied for its biochemical composition, with the main emphasis on intracellular production of dimethylsulfoniopropionate (DMSP). DMSP is suggested to replace nitrogen containing solutes under conditions of nitrogen deficiency. Batch cultures of Antarctic Phaeocystis sp. were grown under iron-rich and iron-poor conditions and simultaneously subjected to high and low light intensities. Iron depletion induced chlorosis and suppressed growth rates as well as the maximum yield of the cultures; these effects were reinforced by low light intensities. Cell volumes were strongly reduced under iron-limited conditions. However, this reduction in cell volume was accompanied by a reduced DMSP content entry in cultures experiencing low light intensities. Under high light conditions, no reduction of DMSP was observed; hence, intracellular DMSP concentrations increased. These observations are discussed relative to carbon and nitrogen metabolism and the biosynthetic pathway of DMSP. It is argued that under high light, low iron conditions, the cells were bordering on nitrogen deficiency induced by iron limitation, whereas under low light, low iron conditions, the cells were energy limited resulting in overall suppressed metabolic rates. Between treatments, DMSP to chlorophyll-a ratios varied by a factor of 5, demonstrating the dependence of this parameter on the physiological state of the cell. |
format |
Article in Journal/Newspaper |
author |
Stefels, J van Leeuwe, M A |
author_facet |
Stefels, J van Leeuwe, M A |
author_sort |
Stefels, J |
title |
Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae):I. Intracellular DMSP concentrations |
title_short |
Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae):I. Intracellular DMSP concentrations |
title_full |
Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae):I. Intracellular DMSP concentrations |
title_fullStr |
Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae):I. Intracellular DMSP concentrations |
title_full_unstemmed |
Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae):I. Intracellular DMSP concentrations |
title_sort |
effects of iron and light stress on the biochemical composition of antarctic phaeocystis sp. (prymnesiophyceae):i. intracellular dmsp concentrations |
publishDate |
1998 |
url |
https://hdl.handle.net/11370/a428cbd5-a866-4b1e-837b-6d92e47c4cae https://research.rug.nl/en/publications/a428cbd5-a866-4b1e-837b-6d92e47c4cae |
geographic |
Antarctic Pacific |
geographic_facet |
Antarctic Pacific |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Stefels , J & van Leeuwe , M A 1998 , ' Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae) : I. Intracellular DMSP concentrations ' , Journal of Phycology , vol. 34 , no. 3 , pp. 486-495 . |
op_relation |
https://research.rug.nl/en/publications/a428cbd5-a866-4b1e-837b-6d92e47c4cae |
op_rights |
info:eu-repo/semantics/restrictedAccess |
_version_ |
1802638086758203392 |