Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton
In situ viability of oceanic phytoplankton may be relatively low in open oceans. This is assumed to be related to the high-irradiance and low-nutrient conditions typical for oligotrophic regions. However, experimental evidence for this phenomenon was not yet available. In the present study, the impo...
Published in: | Limnology and Oceanography |
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Language: | English |
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2013
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Online Access: | https://hdl.handle.net/11370/a416d43c-30ae-43a2-b9ae-37586e10c950 https://research.rug.nl/en/publications/a416d43c-30ae-43a2-b9ae-37586e10c950 https://doi.org/10.4319/lo.2013.58.5.1747 |
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ftunigroningenpu:oai:pure.rug.nl:publications/a416d43c-30ae-43a2-b9ae-37586e10c950 2024-06-02T08:11:34+00:00 Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton Kulk, Gemma van de Poll, Willem H. Visser, Ronald J. W. Buma, Anita G. J. 2013-09 https://hdl.handle.net/11370/a416d43c-30ae-43a2-b9ae-37586e10c950 https://research.rug.nl/en/publications/a416d43c-30ae-43a2-b9ae-37586e10c950 https://doi.org/10.4319/lo.2013.58.5.1747 eng eng https://research.rug.nl/en/publications/a416d43c-30ae-43a2-b9ae-37586e10c950 info:eu-repo/semantics/closedAccess Kulk , G , van de Poll , W H , Visser , R J W & Buma , A G J 2013 , ' Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton ' , Limnology and Oceanography , vol. 58 , no. 5 , pp. 1747-1760 . https://doi.org/10.4319/lo.2013.58.5.1747 NORTH-ATLANTIC OCEAN MARINE DIATOM NITROGEN LIMITATION PACIFIC-OCEAN CELL-DEATH CYANOBACTERIUM PROCHLOROCOCCUS CHLOROPHYLL FLUORESCENCE EMILIANIA-HUXLEYI PHOTOSYSTEM-II IRON STRESS article 2013 ftunigroningenpu https://doi.org/10.4319/lo.2013.58.5.1747 2024-05-07T19:05:36Z In situ viability of oceanic phytoplankton may be relatively low in open oceans. This is assumed to be related to the high-irradiance and low-nutrient conditions typical for oligotrophic regions. However, experimental evidence for this phenomenon was not yet available. In the present study, the importance of nutrient availability in high-irradiance-induced viability loss was therefore studied for three key oceanic phytoplankton species. Prochlorococcus marinus, Ostreococcus sp., and Thalassiosira oceanica were acclimated to two different N:P ratios. Growth, viability, and photophysiology were assessed under nutrient-replete and N- and P-starved conditions. Simultaneously, high-irradiance-induced photoinhibition and viability loss were measured and three inhibitors were used to investigate the underlying physiological mechanisms contributing to viability loss. High-irradiance exposure caused viability loss in P. marinus and Ostreococcus sp., but not in T. oceanica. Low-nutrient availability enhanced survival during high-irradiance exposure, although species-specific differences were observed. The lower sensitivity to high-irradiance intensities at low-nutrient availability was related to conformational changes in photosystem II in P. marinus, to enhanced photoprotection by the xanthophyll pigment cycle and alternative electron transport in Ostreococcus sp., and to enhanced photoprotection by the xanthophyll pigment cycle in T. oceanica. Climate change may lead to enhanced stratification in the open ocean. The resulting increase in the average irradiance intensity phytoplankton experience may promote viability loss in the smallest phytoplankton size fraction. However, this effect may partially be counteracted by the simultaneously expected decrease in nutrient availability. Article in Journal/Newspaper North Atlantic University of Groningen research database Pacific Limnology and Oceanography 58 5 1747 1760 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
NORTH-ATLANTIC OCEAN MARINE DIATOM NITROGEN LIMITATION PACIFIC-OCEAN CELL-DEATH CYANOBACTERIUM PROCHLOROCOCCUS CHLOROPHYLL FLUORESCENCE EMILIANIA-HUXLEYI PHOTOSYSTEM-II IRON STRESS |
spellingShingle |
NORTH-ATLANTIC OCEAN MARINE DIATOM NITROGEN LIMITATION PACIFIC-OCEAN CELL-DEATH CYANOBACTERIUM PROCHLOROCOCCUS CHLOROPHYLL FLUORESCENCE EMILIANIA-HUXLEYI PHOTOSYSTEM-II IRON STRESS Kulk, Gemma van de Poll, Willem H. Visser, Ronald J. W. Buma, Anita G. J. Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton |
topic_facet |
NORTH-ATLANTIC OCEAN MARINE DIATOM NITROGEN LIMITATION PACIFIC-OCEAN CELL-DEATH CYANOBACTERIUM PROCHLOROCOCCUS CHLOROPHYLL FLUORESCENCE EMILIANIA-HUXLEYI PHOTOSYSTEM-II IRON STRESS |
description |
In situ viability of oceanic phytoplankton may be relatively low in open oceans. This is assumed to be related to the high-irradiance and low-nutrient conditions typical for oligotrophic regions. However, experimental evidence for this phenomenon was not yet available. In the present study, the importance of nutrient availability in high-irradiance-induced viability loss was therefore studied for three key oceanic phytoplankton species. Prochlorococcus marinus, Ostreococcus sp., and Thalassiosira oceanica were acclimated to two different N:P ratios. Growth, viability, and photophysiology were assessed under nutrient-replete and N- and P-starved conditions. Simultaneously, high-irradiance-induced photoinhibition and viability loss were measured and three inhibitors were used to investigate the underlying physiological mechanisms contributing to viability loss. High-irradiance exposure caused viability loss in P. marinus and Ostreococcus sp., but not in T. oceanica. Low-nutrient availability enhanced survival during high-irradiance exposure, although species-specific differences were observed. The lower sensitivity to high-irradiance intensities at low-nutrient availability was related to conformational changes in photosystem II in P. marinus, to enhanced photoprotection by the xanthophyll pigment cycle and alternative electron transport in Ostreococcus sp., and to enhanced photoprotection by the xanthophyll pigment cycle in T. oceanica. Climate change may lead to enhanced stratification in the open ocean. The resulting increase in the average irradiance intensity phytoplankton experience may promote viability loss in the smallest phytoplankton size fraction. However, this effect may partially be counteracted by the simultaneously expected decrease in nutrient availability. |
format |
Article in Journal/Newspaper |
author |
Kulk, Gemma van de Poll, Willem H. Visser, Ronald J. W. Buma, Anita G. J. |
author_facet |
Kulk, Gemma van de Poll, Willem H. Visser, Ronald J. W. Buma, Anita G. J. |
author_sort |
Kulk, Gemma |
title |
Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton |
title_short |
Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton |
title_full |
Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton |
title_fullStr |
Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton |
title_full_unstemmed |
Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton |
title_sort |
low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton |
publishDate |
2013 |
url |
https://hdl.handle.net/11370/a416d43c-30ae-43a2-b9ae-37586e10c950 https://research.rug.nl/en/publications/a416d43c-30ae-43a2-b9ae-37586e10c950 https://doi.org/10.4319/lo.2013.58.5.1747 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Kulk , G , van de Poll , W H , Visser , R J W & Buma , A G J 2013 , ' Low nutrient availability reduces high-irradiance-induced viability loss in oceanic phytoplankton ' , Limnology and Oceanography , vol. 58 , no. 5 , pp. 1747-1760 . https://doi.org/10.4319/lo.2013.58.5.1747 |
op_relation |
https://research.rug.nl/en/publications/a416d43c-30ae-43a2-b9ae-37586e10c950 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.4319/lo.2013.58.5.1747 |
container_title |
Limnology and Oceanography |
container_volume |
58 |
container_issue |
5 |
container_start_page |
1747 |
op_container_end_page |
1760 |
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1800757758473011200 |