Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota
The microbiome is essential for development, health and homeostasis throughout an animal's life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carrier...
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ftunigroningenpu:oai:pure.rug.nl:publications/9fd9c05c-3ee0-4a3e-a9f4-f021621a1374 2024-06-02T07:54:28+00:00 Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota van Veelen, H Pieter J Salles, Joana Falcão Tieleman, B Irene 2018-05 application/pdf https://hdl.handle.net/11370/9fd9c05c-3ee0-4a3e-a9f4-f021621a1374 https://research.rug.nl/en/publications/9fd9c05c-3ee0-4a3e-a9f4-f021621a1374 https://doi.org/10.1038/s41396-018-0067-3 https://pure.rug.nl/ws/files/64975218/s41396_018_0067_3.pdf eng eng https://research.rug.nl/en/publications/9fd9c05c-3ee0-4a3e-a9f4-f021621a1374 info:eu-repo/semantics/openAccess van Veelen , H P J , Salles , J F & Tieleman , B I 2018 , ' Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota ' , The ISME journal , vol. 12 , no. 5 , pp. 1375-1388 . https://doi.org/10.1038/s41396-018-0067-3 TRANS-SHELL INFECTION GUT MICROBIOTA EGG VIABILITY BACTERIAL INCUBATION LIFE TRANSMISSION COMMUNITIES DIVERSITY EVOLUTION article 2018 ftunigroningenpu https://doi.org/10.1038/s41396-018-0067-3 2024-05-07T20:15:45Z The microbiome is essential for development, health and homeostasis throughout an animal's life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carriers of the maternal microbiome, warranting characterisation of egg microbiome assembly. Here, we investigated maternal and environmental contributions to avian eggshell microbiota in wild passerine birds: woodlark Lullula arborea and skylark Alauda arvensis. Using 16S rRNA gene sequencing, we demonstrated in both lark species, at the population and within-nest levels, that bacterial communities of freshly laid eggs were distinct from the female cloacal microbiome. Instead, soil-borne bacteria appeared to thrive on freshly laid eggs, and eggshell microbiota composition strongly resembled maternal skin, body feather and nest material communities, sources in direct contact with laid eggs. Finally, phylogenetic structure analysis and microbial source tracking underscored species sorting from directly contacting sources rather than in vivo-transferred symbionts. The female-egg-nest system allowed an integrative assessment of avian egg microbiome assembly, revealing mixed modes of symbiont acquisition not previously documented for vertebrate eggs. Our findings illuminated egg microbiome origins, which suggested a limited potential of eggshells for transgenerational transmission, encouraging further investigation of eggshell microbiome functions in vertebrates. Article in Journal/Newspaper Alauda arvensis University of Groningen research database The ISME Journal 12 5 1375 1388 |
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Open Polar |
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University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
TRANS-SHELL INFECTION GUT MICROBIOTA EGG VIABILITY BACTERIAL INCUBATION LIFE TRANSMISSION COMMUNITIES DIVERSITY EVOLUTION |
spellingShingle |
TRANS-SHELL INFECTION GUT MICROBIOTA EGG VIABILITY BACTERIAL INCUBATION LIFE TRANSMISSION COMMUNITIES DIVERSITY EVOLUTION van Veelen, H Pieter J Salles, Joana Falcão Tieleman, B Irene Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
topic_facet |
TRANS-SHELL INFECTION GUT MICROBIOTA EGG VIABILITY BACTERIAL INCUBATION LIFE TRANSMISSION COMMUNITIES DIVERSITY EVOLUTION |
description |
The microbiome is essential for development, health and homeostasis throughout an animal's life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carriers of the maternal microbiome, warranting characterisation of egg microbiome assembly. Here, we investigated maternal and environmental contributions to avian eggshell microbiota in wild passerine birds: woodlark Lullula arborea and skylark Alauda arvensis. Using 16S rRNA gene sequencing, we demonstrated in both lark species, at the population and within-nest levels, that bacterial communities of freshly laid eggs were distinct from the female cloacal microbiome. Instead, soil-borne bacteria appeared to thrive on freshly laid eggs, and eggshell microbiota composition strongly resembled maternal skin, body feather and nest material communities, sources in direct contact with laid eggs. Finally, phylogenetic structure analysis and microbial source tracking underscored species sorting from directly contacting sources rather than in vivo-transferred symbionts. The female-egg-nest system allowed an integrative assessment of avian egg microbiome assembly, revealing mixed modes of symbiont acquisition not previously documented for vertebrate eggs. Our findings illuminated egg microbiome origins, which suggested a limited potential of eggshells for transgenerational transmission, encouraging further investigation of eggshell microbiome functions in vertebrates. |
format |
Article in Journal/Newspaper |
author |
van Veelen, H Pieter J Salles, Joana Falcão Tieleman, B Irene |
author_facet |
van Veelen, H Pieter J Salles, Joana Falcão Tieleman, B Irene |
author_sort |
van Veelen, H Pieter J |
title |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_short |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_full |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_fullStr |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_full_unstemmed |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_sort |
microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
publishDate |
2018 |
url |
https://hdl.handle.net/11370/9fd9c05c-3ee0-4a3e-a9f4-f021621a1374 https://research.rug.nl/en/publications/9fd9c05c-3ee0-4a3e-a9f4-f021621a1374 https://doi.org/10.1038/s41396-018-0067-3 https://pure.rug.nl/ws/files/64975218/s41396_018_0067_3.pdf |
genre |
Alauda arvensis |
genre_facet |
Alauda arvensis |
op_source |
van Veelen , H P J , Salles , J F & Tieleman , B I 2018 , ' Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota ' , The ISME journal , vol. 12 , no. 5 , pp. 1375-1388 . https://doi.org/10.1038/s41396-018-0067-3 |
op_relation |
https://research.rug.nl/en/publications/9fd9c05c-3ee0-4a3e-a9f4-f021621a1374 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41396-018-0067-3 |
container_title |
The ISME Journal |
container_volume |
12 |
container_issue |
5 |
container_start_page |
1375 |
op_container_end_page |
1388 |
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1800740523357503488 |